Chemical Characterization of Macrophage Cytotoxicity Factor, Macrophage Migration Inhibitory Factor, T-Helper Cell-Replacing Factor and Colony-Stimulating Factor from Culture Supernatants of Concanavalin A-Stimulated Murine Spleen Cells

Hübner, L.; Kniep, Eva; Laukel, H.; Sorg, Clemens; Fischer, Herbert; Gassel, W. D.; Havemann, K.; Kickhöfen, B.; Lohmann‐Matthes, Marie‐Luise; Schimpl, Anneliese; Wecker, Eberhard

doi:10.1016/s0171-2985(80)80098-2

Cited by 14 publications

(4 citation statements)

References 24 publications

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“…Purification of macrophage cytotoxicity factor (MCF) as described by Hübner et al (1980) has not yet been attempted. Therefore it could not be decided whether the striking LK-induced morphological alterations of macrophages were induced by MCF or interferon (IF), or by other factors like colony stimulating factor (CSF).…”

Section: Discussionmentioning

confidence: 99%

“…One of the most interesting effects of activated macrophages is their nonspecific cytotoxicity against proliferating target cells (Lohmann- Matthes, 1976;Keller, 1976;Weinberg et al, 1978). Some of the lymphokines released into the supernatant of Concanavalin A(ConA)-stimulated murine spleen cells, including the macrophage cytotoxicity factor (MCF), have recently been purified and chemically characterised by Hübner et al (1980). Almost all significant research on properties of macrophages in vitro has been done in rodent cell systems.…”

Section: Introductionmentioning

confidence: 99%

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Growth inhibition of marek's disease T‐lymphoblastoid cell lines by chicken bone‐marrow‐derived macrophages activated in vitro

Bülow

Klasen²

1983

Avian Pathology

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Growth inhibition of marek's disease T‐lymphoblastoid cell lines by chicken bone‐marrow‐derived macrophages activated in vitro

Bülow

Klasen²

1983

Avian Pathology

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“…While there is still a widespread belief that MIF and MAF are different manifesta tions of the same molecule, evidence has come out recently that they are not ident ical. [17,18]. On the basis of our model we would predict that differentiation, activa tion and homeostasis of the mononuclear phagocytic system is regulated by a com plex system of lymphokines and other fac tors.…”

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confidence: 96%

Cellular and Molecular Aspects of Lymphocyte Macrophage Interactions

Sorg¹,

Neumann²

1981

Int Arch Allergy Immunol

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“…MQ migration inhibitory factor (MIF), the first lymphokine described [3, 41, is still only defined by bioassay system. It has no apparent relationship to other lymphokine activities such as M@ activating factors [5]. While some progress has been made on the physicochemical characterization of MIF activities from different species [6, 71, the biological function of MIF is still little understood.…”

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confidence: 99%

Functional characteristics of murine macrophages responding to migration inhibitory factors

1984

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Mouse bone marrow cells differentiate in culture in the presence of L cell-conditioned medium to macrophages (M phi). Proliferation, release of plasminogen activator, expression of transglutaminase, random motility and response to a standard preparation of purified M phi migration inhibitory factor (MIF) was recorded daily up to 14 days. After an initial phase of proliferation, precursor cells differentiated into M phi. In the course of maturation, plasminogen activator production was transiently expressed between day 4 and 12; beginning on day 5 the cells expressed intracellular transglutaminase. Random motility of cells was high at the beginning of culture but steadily declined thereafter. The response to MIF was only expressed between day 5 and 8. However, it was possible to induce MIF responsiveness in mature, unresponsive M phi by the addition of L cell-conditioned medium. To characterize the MIF-responsive M phi type further, bone marrow-derived M phi at day 6 of culture were separated on a hypotonic Percoll gradient into three distinct cell bands. While all densities of M phi displayed random migration, only cells with a density between 1.060 and 1.065 were responsive to MIF. We conclude that the response of M phi to MIF is a phenotypic trait transiently expressed in the course of maturation.

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Chemical Characterization of Macrophage Cytotoxicity Factor, Macrophage Migration Inhibitory Factor, T-Helper Cell-Replacing Factor and Colony-Stimulating Factor from Culture Supernatants of Concanavalin A-Stimulated Murine Spleen Cells

Cited by 14 publications

References 24 publications

Growth inhibition of marek's disease T‐lymphoblastoid cell lines by chicken bone‐marrow‐derived macrophages activated in vitro

Growth inhibition of marek's disease T‐lymphoblastoid cell lines by chicken bone‐marrow‐derived macrophages activated in vitro

Cellular and Molecular Aspects of Lymphocyte Macrophage Interactions

Functional characteristics of murine macrophages responding to migration inhibitory factors

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