Whether different field treatments such as straight cut, swathing, or pre-harvest aid application can influence the canola storage is the critical information for growers. The effect of these different field treatments on the infection and development of microflora on canola seeds with 9%, 11%, and 14% moisture content at 20˚C, 25˚C, 30˚C and 35˚C was determined. To evaluate the microfloral infection and development, concentration of CO 2 and O 2 in 150 g canola bulks were measured every 3 d. At the beginning and end of the study (66 d), the seed moisture content, germination, visible mold, invisible fungal infection, and yellow seed count were measured. The swathed and nature ripened canola had a slightly higher respiration rate at ≥30˚C and ≥ 2 wk than the seeds with other field treatments. The swathed canola had a marginally lower initial germination and higher germination at 66 d. The Glyphosate treated and nature ripened canola seeds had a slightly higher chance of visible model development. However, all these differences were not significant at α = 0.05 level and different field treatments and storage conditions did not influence the yellow seed count.