Chemical characterization of p-hydroxycinnamic diesters extracted from Copernicia prunifera and attenuation of biomarkers in C57BL/6J diabetic mice

Silva, José Ytalo Gomes da; Rodrigues, Paula Alves Salmito; Paim, Raquel Teixeira Terceiro; Holanda, Marcelo Oliveira; Lima, Carla Laíne Silva; Lira, Sandra Machado; Freitas, Thais Vital de; Vieira, Ícaro Gusmão Pinto; Mendes, Francisca Noélia Pereira; Marques, Márcia Maria Mendes; Guedes, Maria Izabel Florindo

doi:10.1016/j.fbio.2021.100961

Cited by 1 publication

(1 citation statement)

References 29 publications

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“…Some livers were fixed with 4% paraformaldehyde, and others were frozen with liquid nitrogen to store in an ultra-low-temperature refrigerator at −70 °C. The doses of AMAEs and metformin used in this study were determined by reference to previous studies and were safe for mice. − All animal experiments complied with the ARRIVE guidelines and were approved by the Ethics Review Committee of Experimental Animal Welfare of Shenyang Agricultural University, China (License no. SYXK < Liao >2021–0010).…”

Section: Methodsmentioning

confidence: 99%

Aronia melanocarpa Anthocyanin Extracts Improve Hepatic Structure and Function in High-Fat Diet-/Streptozotocin-Induced T2DM Mice

Chen

Meng

2022

J. Agric. Food Chem.

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Anthocyanins can prevent and ameliorate type 2 diabetes mellitus (T2DM), but its mechanism of action has not been fully established. IKK/NF-κB and JAK/Stat pathways have multiple effects, triggering T2DM. Liver abnormalities in individuals with T2DM are detrimental to glycemic control. We determined whether anthocyanins could improve the liver of individuals with T2DM using IKK/NF-κB and JAK/Stat. We established a T2DM mouse model using a high-fat diet and streptozotocin and then performed Aronia melanocarpa anthocyanin extracts’ (AMAEs’) administration for 5 weeks. AMAEs improved blood glucose and hyperinsulinemia of T2DM mice. In the liver of AMAE-administered T2DM mice, ROS, IKKβ/NF-κB p65, and JAK2/Stat3/5B signalings were down-regulated, thereby reducing the suppressor of cytokine signaling 3 (SOCS3), iNOS, and inflammatory mediators. AMAE-improved hyperinsulinemia also down-regulated SOCS3 by decreasing p-Stat5B in hepatocytes. AMAEs enhanced glucose uptake and conversion and decreased hepatocyte enlargement and inflammatory cells in the liver of T2DM mice. These indicated that AMAEs could alleviate oxidative stress, insulin resistance, inflammation, and tissue damage in the liver of T2DM mice through inhibiting NF-κB p65 and Stat3/5B.

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