Chemical components of Aconitum barbatum var. puberulum and their cytotoxic and antibacterial activities

Abstract

Nineteen compounds, including seventeen alkaloids O-methylarmepavine (1), (S)-6-methoxy-1-(4-methoxybenzyl)-2-methyl -1,2,3,4-tetrahydroisoquinolin-7-ol (2), (+)-(IR,laR)la-hydroxymagnocurarin (3), (6R,6aS,P)-(+)-corydine (4), (+)-N-methyllaurotetanine (5), magnoflorine (6), 3-hydroxy-1,2-dimethoxy-5-methyl-5H-dibenzoindol-4-one (7), imperialine ( 8), crispine B (9), (S)-1-(3-methoxyphenyl)-N-methylpropan-2-amine (10), methyl 2-(acetamino)benzoate (11), 2-carboxyoxanilic acid methylester (12), 4-[2-(methoxycarbonyl)anilino]-4-oxobutanoic acid methyl ester (13), N-methylcorydaldine (14), N-methyl-6,7dimethoxyisoquinolone ( 15), (5S,6R,7S,8R)-5-amino-(2Z,4Z)-1,2,3-trihybuta-2,4-dienyloxy-pentane-6,7,8,9-tetraol ( 16), nicotinic acid (17), and two megastigmane type compounds, S(+)dehydrovomifoliol ( 18) and megastigmane ( 19), were isolated from the Aconitum barbatum var.puberulum Ledeb. Compounds 1-3 and 5-19 were isolated from this plant for the first time, of which compound 11 was isolated from natural source for the first time. Cytotoxicity evaluation revealed that compound 5 displayed mild cytotoxicity against the Hela cell lines (IC 50 13.69 ± 0.036μM).Antibacterial activity evaluation revealed that compounds 1 and 6 showed strong antibacterial activity against the Gram-positive bacterium, S. aureus.

Chemical Constituents of the Mushroom Dictyophora indusiata and Their Anti-Inflammatory Activities

“…On the basis of the comprehensive analysis of spectroscopic data from high resolution electrospray ionization mass spectra (HRESIMS), 1D/2D nuclear magnetic resonance (NMR) spectra, ultraviolet (UV) spectra, and comparison with the references, four previously undescribed compounds were named Indusiataines A–D ( 1 – 4 ). Fourteen known compounds were elucidated to be 5-(Methoxymethyl)-1 H -pyrrole-2-carbaldehyde ( 5 ) [ 21 ], nicotinic acid ( 6 ) [ 22 ], dibutyl phthalate ( 7 ) [ 23 ], 5-hydroxymethyl-2-furancarboxaldehyde ( 8 ) [ 24 ], adenosine ( 9 ) [ 25 ], oxindole ( 10 ) [ 26 ], 3-oxo-4-benzyl-3,4-dihydro-1 H -pyrrolo [2,1-c] oxazine-6-methylal ( 11 ) [ 27 ], 2-methyl-3-pyridinol ( 12 ) [ 28 ], linoleic acid ( 13 ) [ 29 ], oleic acid ( 14 ) [ 30 ], 1(10→6)abeo-ergosta-5,7,9,22-tetraen-11β-methoxy-3α-ol ( 15 ) [ 31 ], citreoanthrasteroid ( 16 ) [ 31 ], 5α,6α-epoxy-3β-hydroxy-(22 E )-ergosta-8(14),22-dien-7-one ( 17 ) [ 32 ], and 7-ketositosterol ( 18 ) [ 33 ] ( Figure 1 ). The UV, HRESIMS, and NMR spectra of compounds 1 – 4 are provided in Figure S1 .…”

High‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry for profiling diterpenoid alkaloids in Aconitum species

“…Studies on the secondary metabolites of natural sources of animals, plants, or microorganisms are still one of the important contents of modern drug research [25–27]. Diterpenoid alkaloids have long attracted the interest of chemists and pharmacologists because of their complex chemical structures and toxicity [7–9, 23, 16, 28–30]. In previous studies, more than 1000 diterpene alkaloids were discovered in Aconitum plants.…”

“…It is recorded in “flora of China” as having antispasmodic, analgesic, dispelling wind and dampness, and antipyretic effects [3–5]. However, the root tubers of most aconite plants are highly toxic [6–10], as well as other toxic components [11–12]. So far, More than 1000 diterpene alkaloids have been isolated from the alcoholic or aqueous extracts of the tuberous roots of the aconite plant.…”

Content Determination and Release Characteristics of Six Components in the Different Phases of “Glycyrrhizaglabra-Nux vomica” Decoction by UPLC-MS/MS

“…Decoction is one of the oldest and most common forms of administration of herbal medicine in China. Chinese herbs with medicinal properties, such as Aconitum ( Aconitum carmichaeli Debx L.) [ 1 , 2 ] and Nux vomica ( Strychnos nux-vomica L.) [ 3 , 4 ], are often combined to prepare tonics to reduce toxicity and increase effectiveness [ 5 , 6 , 7 ]. Modern science has found that the high temperature and complex chemistry of the decoction process, together with the dissolved secondary metabolites, including small molecules, biological macromolecules and inorganic elements, alter the physicochemical properties of herbal preparations.…”