Chemical composition and 13C NMR spectroscopic characterisation of ulvans from Ulva (Ulvales, Chlorophyta)

Lahaye, Marc; Cimadevilla, Enrique Alvarez-Cabal; Kuhlenkamp, Ralph; Quéméner, Bernard; Lognoné, Vincent; Dion, Patrick

doi:10.1007/978-94-011-4449-0_63

Cited by 20 publications

(34 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been shown that the ulvan composition can slightly vary in samples harvested at different location or during various time periods (Lahaye et al 1999;Robic et al 2009a, b). Consequently, significant variations in term of uronic acid contents were observed in the samples that we prepared.…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

An Ulva armoricana extract protects plants against three powdery mildew pathogens

et al. 2011

View full text Add to dashboard Cite

The protective activity of a crude extract prepared from the green macroalga, Ulva armoricana, previously shown to induce plant defence responses, was evaluated on three plant species, common bean, grapevine and cucumber, cultivated in the greenhouse and inoculated with three powdery mildew pathogens Erysiphe polygoni, E. necator and Sphareotheca fuliginea respectively. Chemical analyses showed that the extract was enriched in ulvans, which are green algae polysaccharides essentially composed of uronic acid and sulphated rhamnose. Weekly applications were performed by spraying of the green algal extract at various dilutions on bean, grapevine and cucumber leaves. A significant effect (50% protection) was observed using a dilution corresponding to about 3 gl −1 dry matter and up to 90% reduction of symptom severity was obtained for the highest concentration (1/9 dilution, 6 gl −1 dry matter) for the three plant species. To study the natural variability of the protective activity, five extracts prepared from algae batches harvested at different year periods were evaluated. Although polysaccharide composition varied among batches, all extracts elicit a reporter gene regulated by a defence-gene promoter in a transgenic tobacco line, and protect cucumber plants against powdery mildew infection. Together, these data demonstrate that U. armoricana is a reproducible source of active compounds which can be used to efficiently protect crop plants against powdery mildew diseases.

show abstract

Section: Discussionmentioning

confidence: 97%

“…Rhamnose 3-sulphate can be linked to xylose and/or to xylose 2-sulphate. Additionally, rhamnose 3-sulphate can be partially branched at C-2 by single side-chains of glucuronic acid (Lahaye et al 1997(Lahaye et al , 1999. Purified ulvan induced defence gene expression via the jasmonic acid pathway (Jaulneau et al 2010).…”

mentioning

confidence: 99%

An Ulva armoricana extract protects plants against three powdery mildew pathogens

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Moreover, it has been used as a drug in traditional Chinese medicine for hyperlipidemia, sunstroke and urinary diseases, etc. Polysaccharide extracted from U. pertusa is a heteropolysaccharide, mainly composed of rhamnose, xylose, glucose, glucuronic acid, iduronic acid and sulfate, with smaller amounts of mannose, arabinose and galactose (Quemener et al, 1997;Ray & Lahaye, 1995;Lahaye et al, 1999). The main repeating disaccharide units are [ß-D-GlcpA-(1−>4)-α-L-Rhap 3s] and [α-L-Idop A-(1−>4)-α-L-Rhap 3s] (Lahaye et al, 1999;Lahaye, 1998).…”

Section: Introductionmentioning

confidence: 98%

Antioxidant activity of different molecular weight sulfated polysaccharides from Ulva pertusa Kjellm (Chlorophyta)

et al. 2005

View full text Add to dashboard Cite

Polysaccharides extracted from Ulva pertusa Kjellm (Chlorophyta) are a group of sulfated heteropolysaccharides, the ulvans. In this study, different molecular weight ulvans were prepared by H 2 O 2 degradation and their antioxidant activities investigated including superoxide and hydroxyl radical scavenging activity, reducing power and metal chelating ability. The molecular weights of natural and degraded ulvans were 151.7, 64.5, 58.0, and 28.2 kDa, respectively, as determined by high performance gel permeation chromatography. Among the four samples, U 3 (the lowest molecular weight sample) showed significant inhibitory effects on superoxide and hydroxyl radicals with IC 50 values of 22.1 μg mL −1 and 2.8 mg mL −1 ; its reducing power and metal chelating ability were also the strongest among the four samples. All the other samples also demonstrated strong activity against superoxide radicals. The results indicated that molecular weight had a significant effect on the antioxidant activity of ulvan with low molecular weight ulvan having stronger antioxidant activity.Abbreviations: U, polysaccharide extracted from U. pertusa (ulvan, Mw, 151.7 kDa); U 1 , degraded ulvan of molecular weight 64.5 kDa; U 2 , degraded ulvan of molecular weight 58.0 kDa; U 3 , degraded ulvan of molecular weight 28.2 kDa; NBT, nitro blue tetrazolium; PMS, phenazine methosulfate; NADH, nicotinamide adenine dinucleotidereduced; EDTA, ethylene diamine tetra-acetic acid; TCA, trichloroacetic acid; TBA, thiobarbituric acid; DR, deoxyribose; HPGPC, high performance gel permeation chromatography; GC, gas liquid chromatography.

show abstract

“…It is important to mention also that ulvan, a major Sulfated Polysaccharide (SP) found in the cell wall of green algae, composed mainly of rhamnose, glucuronic acid, iduronic acid, xylose, glucose, sulphate with small amounts of iduronic acid and traces of galactose and represents 8-29% of the algae dry weight [25,26,[44][45][46][47][48][49][50][51]. The presence of the sulfate groups and the unusual chemical composition and structure of ulvan render it different biological properties [26,52,53].…”

Section: Biological Activitiesmentioning

confidence: 99%

“…This complex SP can also be of potential interest for food, pharmaceutical and agricultural applications [26,[51][52][53][54]. In the recent years, ulvan has been extensively investigated for development of novel drugs and functional foods [26,49,53]. Because of its peculiar composition and structure [50], it represents a potential source of new functional biopolymer and it is widely used as biomaterial for tissue engineering in regenerative medicine.…”

Section: Biological Activitiesmentioning

confidence: 99%

The Marine Macroalgae of the Genus Ulva: Chemistry, Biological Activities and Potential Applications

Silva¹,

Vieira²,

Almeida³

et al. 2013

Oceanography

View full text Add to dashboard Cite

Chemical composition and 13C NMR spectroscopic characterisation of ulvans from Ulva (Ulvales, Chlorophyta)

Cited by 20 publications

References 14 publications

An Ulva armoricana extract protects plants against three powdery mildew pathogens

An Ulva armoricana extract protects plants against three powdery mildew pathogens

Antioxidant activity of different molecular weight sulfated polysaccharides from Ulva pertusa Kjellm (Chlorophyta)

The Marine Macroalgae of the Genus Ulva: Chemistry, Biological Activities and Potential Applications

Contact Info

Product

Resources

About