Chemical composition and anti‐complement activity of Glechomae Herba collected in different months

Abstract

Glechomae Herba (GH) is derived from the dried aerial part of Glechoma longituba (Nakai) Kupr., which is harvested from spring to autumn. It has the effects of clearing heat and detoxification. The aim of this paper was to study the chemical composition and the anti‐complement activity of GH collected in different months. Ultra‐high‐performance liquid chromatography coupled to quadrupole time‐of‐flight tandem mass spectrometry based on predicted compounds screening and diagnostic ion filter strategy was developed for identifying the chemical composition of GH collected in different months. A total of 102 compounds—40 chlorogenic acids (CGAs), 32 phenolic acids, and 30 flavonoids—were reasonably identified in GH. Thirty‐four CGAs were discovered in GH for the first time. The correlations between chemical compositions and anti‐complement activities of GH collected in different months were analyzed. Phenolic acids and flavonoids were found to be negatively correlated with anti‐complement activity, and CGAs were positively correlated with anti‐complement activity. At the same time, six CGA standards had obvious anti‐complement activity. It was demonstrated that different harvest months had a significant impact on the difference in chemical composition and anti‐complement activity of GH. And CGAs might play an important role in the anti‐complement activity of GH.

Characterization of Phenolics in Rejected Kiwifruit and Their Antioxidant Potential

“…Two ferulic acid derivatives were identified in kiwifruit samples, including ferulic acid 4-O-glucoside and ferulic acid 4-O-glucuronide. Ferulic acid 4-O-glucuronide was previously reported to be found in glechomae herba by Luo, et al [59].…”

Rapid identification of chemical constituents in Yinqiao powder using ultra‐high‐performance liquid chromatography coupled to quadrupole‐time‐of‐flight tandem mass spectrometry using data filtering strategy

“…Our previous published papers (Li et al, 2019; Luo et al, 2019, 2020) have summarized in detail the identification of chlorogenic acids and flavonoids in TCM. According to the reported DPIs and NL of chlorogenic acids and flavonoids (Supporting Information Figures S6 and S7), 9 chlorogenic acids and 24 flavonoids were identified in Yinqiao powder water decoction.…”

“…Our previous published papers (Li et al, 2019;Luo et al, 2019Luo et al, , 2020 have summarized in detail the identification of chlorogenic acids and of this method was that the structures of new compounds could not be accurately identified. In the future, new compounds would be separated, and nuclear magnetic resonance would be applied to ensure their structures.…”

“…However, due to the complex and huge MS/MS data sets and matrix interference, it is very difficult to identify the chemical components of TCM. To solve this problem, post-acquisition data processes, such as diagnostic product ions (DPIs) and neutral loss (NL) filtering strategy that display higher efficiency to identify structure-related compounds from entire classes of compounds based on the specific DPI and NL and potentially leads to the discovery of new compounds, have been proposed (An et al, 2021;Li et al, 2019;Luo et al, 2019Luo et al, , 2020. The main disadvantage of the DPI and NL filtering strategy is that the analyst must determine which DPI and NL are specific to which class of compounds first.…”

The phytochemistry, pharmacology and traditional medicinal use ofGlechomae Herba– a systematic review

“…In 2019, Luo et al used ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight tandem mass analysis (UHPLC-QTOF-MS/MS) to identify 40 chlorogenic acids. 22 In the same year, Li et al also used this method to identify the chemical constituents such as caffeoylquinic acid, feruloylquinic acid, p -coumaroylquinic acid and di-caffeoylquinic acid in GH. 23 It was concluded that chlorogenic acids had anti-complement activity.…”