Chemical composition and antibacterial activity of 12 medicinal plant ethyl acetate extracts using LC–MS feature‐based molecular networking

Yang, Zi; Li, Li; Chen, Chen‐Hui; Zhang, Yuanyuan; Yang, Yi Yuan; Zhang, Peng; Bao, Guan‐Hu

doi:10.1002/pca.3103

Cited by 18 publications

(12 citation statements)

References 78 publications

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“…Because many plant defense-relates genes were specifically induced in developing PLBs, we hypothesized that the dynamic metabolomic reprogramming of developing PLBs leads to synthesis of antimicrobial metabolites. Ethyl acetate (EtOAc), which is commonly used for plant metabolite extraction ( Tamokou et al., 2012 ; Oliveira et al., 2013 ; Lien et al., 2014 ; Yang et al., 2022 ), was used to prepare PLB crude extract. The crude extract was then tested for its effect on growth of three plant pathogens including Acidovorax citrulli Aac153 (watermelon bacterial fruit blotch disease), Pectobacterium carotovorum subsp.…”

Section: Resultsmentioning

confidence: 99%

Protocorm-like-body extract of Phalaenopsis aphrodite combats watermelon fruit blotch disease

Chen

Huang

et al. 2022

Front. Plant Sci.

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Bacterial fruit blotch, caused by the seedborne gram-negative bacterium Acidovorax citrulli, is one of the most destructive bacterial diseases of cucurbits (gourds) worldwide. Despite its prevalence, effective and reliable means to control bacterial fruit blotch remain limited. Transcriptomic analyses of tissue culture-based regeneration processes have revealed that organogenesis-associated cellular reprogramming is often associated with upregulation of stress- and defense-responsive genes. Yet, there is limited evidence supporting the notion that the reprogrammed cellular metabolism of the regenerated tissued confers bona fide antimicrobial activity. Here, we explored the anti-bacterial activity of protocorm-like-bodies (PLBs) of Phalaenopsis aphrodite. Encouragingly, we found that the PLB extract was potent in slowing growth of A. citrulli, reducing the number of bacteria attached to watermelon seeds, and alleviating disease symptoms of watermelon seedlings caused by A. citrulli. Because the anti-bacterial activity can be fractionated chemically, we predict that reprogrammed cellular activity during the PLB regeneration process produces metabolites with antibacterial activity. In conclusion, our data demonstrated the antibacterial activity in developing PLBs and revealed the potential of using orchid PLBs to discover chemicals to control bacterial fruit blotch disease.

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Section: Resultsmentioning

confidence: 99%

Protocorm-like-body extract of Phalaenopsis aphrodite combats watermelon fruit blotch disease

Chen

Huang

et al. 2022

Front. Plant Sci.

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“…Its use can be expanded to assess the possibility of natural product poisoning in clinical situations [65]. An ultraperformance liquid chromatography-quadrupoletime-of-flight-mass spectrometry has been employed in several studies to characterize the potential components [66]. Additionally, LC-MS analysis revealed the presence of compounds like Mahaleboside (Coumarin glycosides), Chlorogenic acid (Polyphenols), Crotanecine (Pyrrolizidine alkaloids), Perlolyrine (Harmala alkaloids), Phrymarolin I (Benzodioxoles), Diplodiatoxin (Gamma keto acids), Dihydrocapsaicin (Capsaicinoid), Morindone (Anthraquinone), Corchorifatty acid (Linoleic acid), and Polyporusterone B (triterpene carboxylic acid) were known to possess anticancer, depression, hypertension, anti-inflammatory activities [67][68][69][70].…”

Section: Discussionmentioning

confidence: 99%

The promising guide to LC–MS analysis and cholinesterase activity of Luffa cylindrica (L.) fruit using in vitro and in-silico analyses

Singh

Chitra

2023

Futur J Pharm Sci

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Background Luffa cylindrica (L.) is an annual climbing plant that produces fibrous fruit and can also be used as a vegetable in northern parts of India. Various studies have been carried out on the plant and found to have anti-inflammatory, antifungal, analgesic, anti-myocardial, anti-hyper triglyceride, immunostimulant, anti-allergic, and other properties. The ethanolic extract of the Luffa cylindrica (L.) fruit has not yet been subjected to LC–MS analysis for several bioactive chemicals that target neurological diseases. Oxidative stress is an inevitable situation in AD mechanisms and is a key bridge connecting various AD pathways. Results Luffa cylindrica contains various phytochemicals and showed highest alkaloid content of 21.39 ± 1.47 mg of AE/g. A total of 80 compounds were identified in the ethanolic extract from LC–MS analysis. The bioactive compounds were screened for eligibility by Lipinski's rule of five for docking with receptors responsible for causing oxidative stress-associated Alzheimer's disease. Perlolyrine was chosen to perform in-silico docking. An in vitro activity of cholinesterase showed highest inhibition at 500 µg/ml. In-silico docking of perlolyrine showed better binding affinity and score. Results revealed that out of 10 docked receptors, amyloid beta showed the highest binding affinity with an energy of − 46.1 kcal/mol showing promising drug for Alzheimer's disease. Conclusion Based on current findings, the study reports the presence of a promising, bioactive compound (perlolyrine) and in turn provides an optimistic note in exploring its biological activity in vivo with oxidative stress-related Alzheimer's disease.

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“…T.sinensis and T.ciliata have the most noteworthy application in the Toona. Toona sinensis (A. Juss) Roem is a unique species of vegetable in China, its young shoots and leaves are crisp and juicy, fragrant and unique in flavor, it is a traditional and valuable woody vegetable that our people like to eat, and is also a local product for foreign trade export [6,7]. T.sinensis is not only an excellent vegetable but also a natural green nutritious food of medicinal and food origin.…”

Section: Introductionmentioning

confidence: 99%

Sequencing and analysis of the complete mitochondrial genomes of Toona sinensis and Toona ciliata reveal evolutionary features of Toona

Liu

et al. 2023

BMC Genomics

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Background Toona is a critical genus in the Meliaceae, and the plants of this group are an asset for both restorative and restorative purposes, the most flexible of which are Toona sinensis and Toona ciliata. To concentrate on the advancement of mitochondrial(Mt) genome variety in T.sinensis and T.ciliata, the Mt genomes of the two species were sequenced in high throughput independently, after de novo assembly and annotation to construct a Mt genome map for comparison in genome structure. Find their repetitive sequences and analyze them in comparison with the chloroplast genome, along with Maximum-likelihood(ML) phylogenetic analysis with 16 other relatives. Results (1) T. sinensis and T.ciliata are both circular structures with lengths of 683482 bp and 68300 bp, respectively. They share a high degree of similarity in encoding genes and have AT preferences. All of them have the largest Phe concentration and are the most frequently used codons. (2) Both of their Mt genome are highly preserved in terms of structural and functional genes, while the main variability is reflected in the length of tRNA, the number of genes, and the value of RSCU. (3) T. siniensis and T. ciliata were detected to have 94 and 87 SSRs, respectively, of which mononucleotides accounted for the absolute proportion. Besides, the vast majority of their SSRs were found to be poly-A or poly-T. (4)10 and 11 migrating fragments were identified in the comparison with the chloroplast genome, respectively. (5) In the ML evolutionary tree, T.sinensis and T.ciliata clustered individually into a small branch with 100% support, reflecting two species of Toona are very similarly related to each other. Conclusions This research provides a basis for the exploitation of T.sinensis and T.ciliata in terms of medicinal, edible, and timber resources to avoid confusion; at the same time, it can explore the evolutionary relationship between the Toona and related species, which does not only have an important practical value, but also provides a theoretical basis for future hybrid breeding of forest trees, molecular markers, and evolutionary aspects of plants, which has great scientific significance.

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Chemical composition and antibacterial activity of 12 medicinal plant ethyl acetate extracts using LC–MS feature‐based molecular networking

Cited by 18 publications

References 78 publications

Protocorm-like-body extract of Phalaenopsis aphrodite combats watermelon fruit blotch disease

Protocorm-like-body extract of Phalaenopsis aphrodite combats watermelon fruit blotch disease

The promising guide to LC–MS analysis and cholinesterase activity of Luffa cylindrica (L.) fruit using in vitro and in-silico analyses

Sequencing and analysis of the complete mitochondrial genomes of Toona sinensis and Toona ciliata reveal evolutionary features of Toona

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