Chemical Composition and Antioxidant Activities of Three Polysaccharide Fractions from Pine Cones

Abstract

The traditional method of gas chromatography-mass spectrometry for monosaccharide component analysis with pretreatment of acetylation is described with slight modifications and verified in detail in this paper. It was then successfully applied to the quantitative analysis of component monosaccharides in polysaccharides extracted from the pine cones. The results demonstrated that the three pine cone polysaccharides all consisted of ribose, rhamnose, arabinose, xylose, mannose, glucose and galactose in different molar ratios. According to the recovery experiment, the described method was proved accurate and practical for the analysis of pine cone polysaccharides, meeting the need in the field of chemical analysis of Pinus plants. Furthermore; the chemical characteristics, such as neutral sugar, uronic acids, amino acids, molecular weights, and antioxidant activities of the polysaccharides were investigated by chemical and instrumental methods. The results showed that the chemical compositions of the polysaccharides differed from each other, especially in the content of neutral sugar and uronic acid. In the antioxidant assays, the polysaccharide fractions exhibited effective scavenging activities on ABTS radical and hydroxyl radical, with their antioxidant capabilities decreasing in the order of PKP > PAP > PSP. Therefore, although the polysaccharide fractions had little effect on superoxide radical scavenging, they still have potential to be developed as natural antioxidant agents in functional foods or medicine.

Structural Characterization, Antioxidant Activity, and Biomedical Application of Astragalus Polysaccharide Degradation Products

“…The antioxidant reacts with ABTS + to cause the reaction to fade and initiate a change in the absorbance of a particular wavelength segment to determine the size of the antioxidant to remove ABTS free radicals [22]. The ABTS radical scavenging activity of polysaccharides was performed according to [23] with slight modification. 7 mmol/L ABTS solution was mixed with 2.45 mmol/L potassium persulfate aqueous solution, and then the mixture was incubated in the dark at room temperature for 12-16 h. Then 3 mL ABTS + solution was added to 1 mL polysaccharide solutions of various concentrations in a test tube.…”

Physicochemical Properties of Edible Seed Hemicelluloses

“…Hemicellulose hydrolysis and monosaccharide derivatization processes were carried out as described by [23]. Galactose, glucose, mannose and xylose contents were determined using gas chromatography with mass spectrometry detection (GCMS-QP2010, Shimadzu, Tokyo, Japan).…”

Ultrasonication of Polysaccharides from Tunisian Zizyphus lotus Fruit: Emulsifying Capacities, Rheological Properties and Antioxidant activities

“…It's clearly shown that the ultrasound treatment samples (ZLP 30 , ZLP 60 , ZLP 90 and ZLP 120 ) have the same functional groups as the native polysaccharide (ZLP 0 ). In fact, The five samples displayed a broad stretching intense characteristic peak at around 3300 cm −1 which was ascribe to the stretching vibration of hydroxyl group (-OH) attributed to inter and intra molecular interaction of the polysaccharides chains [47]. Besides, there is no change in the absorption bands detected at 1605 cm −1 and 1716 cm −1 which are characteristic of the stretching vibration of C = O which attributed respectively to the carboxylate ion (COO-), and the O-acetyl groups [48,49].…”