Chemical composition and antioxidant activity of Astragalus monspessulanus L. growing in semiarid areas of Algeria

Abstract

This paper reports a phytochemical study of the aerial parts of Astragalus monspessulanus L. growing in Algeria. It deals with the isolation and structure elucidation of 13 known compounds. From the n-butanol extract, seven flavonoids 1-7, two saponins and one lignan were isolated. In addition, two phytosterols and one triterpenoid were isolated from the ethyl acetate extract. The structures of all the isolated products were determined by using 1D and 2D-NMR techniques, measurement of optical rotation, mass spectrometry ESI-MS and by comparison with literature data. Furthermore, the antioxidant activity of the n-butanol extract of the aerial parts of Astragalus monspessulanus L. was investigated using the DPPH radical scavenging and ferrous ion chelating assays. The n-butanol extract showed low (EC 50 = 2.09±0.434 mg mL -1 ) to moderate (IC 50 = 63.60±0.01 μg mL -1 ) antioxidant activities depending on the test method.

Hyphenated LC-ABTS·+ and LC-DAD-HRMS for simultaneous analysis and identification of antioxidant compounds in Astragalus emarginatus Labill. extracts

“…The antioxidant activity observed for AEL extracts is not surprising, because such activity has also been reported in other Astragalus species in previous studies [ [24] , [25] , [26] ]. Our data indicated that the antioxidant activity of AEL could mainly be attributed to polar compounds.…”

Identification and quantification of phenolic compounds of Western Siberia Astragalus danicus in different regions

“…[27], the averaged sample of the aerial part of plants (shoot, leaves, flowers at a ratio of 2:2:1) was collected after drying the field samples to an air-dry state (500 g). The plant material was crushed and subjected to extraction with methyl carbinol (the ratio of plant material/extractant 1:10, temperature 10 °C) for 2 days under constant stirring using a 200×g shaker until complete extraction of biologically active substances [28, 29, 30, 31, 32, 33]. The resulting tincture was a clear liquid of dark greenish-brown color with a characteristic odor.…”

Anticancer Secondary Metabolites: From Ethnopharmacology and Identification in Native Complexes to Biotechnological Studies in Species of Genus Astragalus L. and Gloriosa L.

“…One novel quercetin tetraglycoside and eight known flavonoids are isolated as well [ 53 ]. Also, from the aerial parts of this species, two saponins are reported [ 64 ]. Two rare flavonoids with an unusual hydroxymethylglutaric acid as a moiety: quercetin-3- O - α -L-rhamnopyranosyl-(1→2)-[6- O -(3-hydroxy-3-methylglutaryl)- β -D-galactopyranoside and kaempferol-3- O - α -L-rhamnopyranosyl-(1→2)-[6- O -(3-hydroxy-3-methylglutaryl)- β -D-galactopyranoside are isolated from the aerial parts of A. monspessulanus subsp.…”