Chemical Composition and Antioxidant Activity of Essential Oil and Organic Extracts of Premna integrifolia Linn

Al-Reza, Sharif M.; Rokonuzzaman, M.; Afroz, Monira; Hussain, Md. Iqbal; Rashid, Mamunur; Rahman, Atiqur

doi:10.1590/1678-4324-2016160223

Cited by 8 publications

(8 citation statements)

References 19 publications

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“…Anticoagulant, antiarthritic, antihyperglycemic, and antimicrobial characteristics have also been reported for the plant [ 15 ]. It protects against cardiovascular diseases since it contains alkaloids and iridoid glycosides [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Topical Application of Premna integrifolia Linn on Skin Wound Injury in Rats Accelerates the Wound Healing Process: Evidence from In Vitro and In Vivo Experimental Models

Alsareii

Alzerwi

AlAsmari

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. When the skin and tissues within the body are injured, the healing process begins. Medicinal herbs have been used to cure wounds since time immemorial. The antimicrobial and antioxidant activity possessed by P. integrifolia may accelerate wound healing. Objectives. To assess the wound healing activity of Premna integrifolia extract (PIE) by employing in-vivo experimental animal models and an in-vitro migration scratch assay. Furthermore, to assess its cytotoxicity using the MTT assay. Methods. Wistar albino rats were used for the in vivo wound healing models. The animals were divided into four groups at random: Group I was untreated. Group II was vehicle control (ointment base). Group III was PIE ointment (5% W/W). Group IV was standard (povidone-iodine ointment) (5% W/W). The ointments were applied directly to the wounds as described above until they healed completely. The wound contraction percentage and tensile strength were calculated. The MTT test was used to determine the viability of the test extract against the fibroblast cells. The scratch assay was used in vitro to determine the wound healing potential of the test drug. P ≤ 0.05 values were considered statistically significant. Results. Premna integrifolia extract did not possess any noticeable cytotoxicity to the cell line and showed an IC50 of 185.98 μg/ml. The wound contraction potential of PIE ointment-treated animals was considerably greater ( P ≤ 0.001 ) on days 4, 8, 12, 16, and 20 when compared to the control group. The percentage of wound contraction on day 20 was 99.92% in PIE-treated animals compared to 83.23% in untreated animals. Compared to the untreated group, the duration of full epithelization was significantly ( P ≤ 0.01 ) shorter in the test group. When compared to the incision control group, the animals treated with PIE ointment had significantly higher ( P ≤ 0.001 ) tensile strength. In addition, animals given the test drug had a significant ( P ≤ 0.001 ) increase in total protein and hydroxyproline. In the in vitro scratch assay, test drug-treated cells demonstrated greater cell migration. Histology images confirmed that the test drug-treated group had epithelial tissue proliferation and keratinization. Conclusion. The current study found that Premna integrifolia improved wound healing activity both in vitro and in vivo. These findings indicate that Premna integrifolia extract has wound-healing potential and could be a viable source of nutraceuticals with wound-healing properties.

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Section: Introductionmentioning

confidence: 99%

Topical Application of Premna integrifolia Linn on Skin Wound Injury in Rats Accelerates the Wound Healing Process: Evidence from In Vitro and In Vivo Experimental Models

Alsareii

Alzerwi

AlAsmari

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…Active oxygen free radicals are intermediate metabolites produced in life activities (Tahmouzi & Ghodsi, 2014). Excessive reactive oxygen free radicals can cause damage to macromolecular substances in organisms, induce various diseases, accelerate the aging of body, and thus seriously affect human health (Al-Reza et al, 2016;Han et al, 2019;Mo et al, 2017;Ru et al, 2019). In order to reduce the damage of active oxygen radicals, antioxidants are commonly used.…”

Section: Introductionmentioning

confidence: 99%

Extraction optimization and antioxidant activity of Phyllanthus urinaria polysaccharides

Liu

2021

Food Sci. Technol

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Three extraction parameters including extraction time, material to solvent ratio, and extraction temperature for the extraction yield of Phyllanthus urinaria polysaccharides were optimized by response surface methodology. The results revealed that the optimal extraction process was extraction time of 5.3 h, extraction temperature of 93 °C, and material to solvent ratio of 1:47 g/mL, under which the polysaccharide yield was 6.40%; The three parameters had significant effect on the polysaccharide yield, and the influence of extraction time was the greatest and that of extraction temperature was the lowest. The antioxidant activity test indicated that the equivalent scavenging ability of Phyllanthus urinaria polysaccharides on DPPH and ABTS free radicals was 0.88 and 0.84 μg TBHQ/μg of polysaccharides, respectively; while the reducing power and total antioxidant capacity of Phyllanthus urinaria polysaccharides was equivalent to 93.62% and 93.55% of TBHQ, respectively. The Phyllanthus urinaria polysaccharides had good antioxidant activity.

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“…The free radical scavenging and antioxidant potential of the extract was evaluated using DPPH according to the procedure described by Al-Reza et al [38] with minor modification. The percentage of the radical inhibition activity was evaluated based on the following expression:…”

Section: (Ii) Evaluation Of 22ʹ-diphenyl-1-picryl-hydrazyl (Dpph) Radical Scavenging Assaymentioning

confidence: 99%

Secondary Metabolites, Pharmacognostic and Therapeutic Activities of the Rhizome Extract of Curcuma longa Grown in South-West, Nigeria

S¹,

O²,

Anuoluwa³

et al. 2020

J Phytopharmacol

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Curcuma longa is an important plant with diverse medicinal properties. The aim of this study was to determine the chemical constituents and phenolic contents, ascorbic acid content, antioxidant, anti-arthritic and antibacterial potential of the rhizome extract of C. longa grown in South-West, Nigeria. GC-MS, UV-Visible Spectrophotometer, other established biochemical methods were used to investigate the rhizome extract. The GC-MS analysis revealed the presence of sixty ( 60) therapeutically active phytochemicals. The most abundant components were: nopol (23.0%), megastigma-3,7(E),9-triene (9.0%), curlone (7.0%) and ( 4E)-2-methyl-4-octenal (5.9%). The percentage of α-curcume, α-zingiberene and βsesquiphellandrene were 0.9, 1.6 and 1.9%, respectively. The polyphenol, flavonoid and ascorbic acid values were 3,270.30 ±0.00 µgmg -1 GAE, 51.79±0.00 µgmg -1 QE and 51.48±0.03 µgmg -1 AAE, respectively. The rhizome extract of C. longa contained some phenolic compounds such as o-guaiacol (1.5%), p-vinylguaiacol (2.0%) and ferulic acid methyl ester (1.5%). The rhizome extract showed very low IC50 and therefore high AAI with Galvinoxyl (25.0 µgml -1 and 1.68) and DPPH (5.0 µgml -1 and 8.0). The total antioxidant capacity value was 1,173.08±0.00 µgmg -1 AAE. The rhizome extract also gave high anti-arthritic protein denaturation value between 86.82-69.32%. The extract was active against all the tested bacteria isolates showing high zones of inhibition (10.0-24.0 mm). The studies provide important information for efficient and effective uses of rhizome of C. longa for nutritional and therapeutic purposes.

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Chemical Composition and Antioxidant Activity of Essential Oil and Organic Extracts of Premna integrifolia Linn

Abstract: This study was designed to examine the chemical composition and in vitro antioxidant

Cited by 8 publications

References 19 publications

Topical Application of Premna integrifolia Linn on Skin Wound Injury in Rats Accelerates the Wound Healing Process: Evidence from In Vitro and In Vivo Experimental Models

Topical Application of Premna integrifolia Linn on Skin Wound Injury in Rats Accelerates the Wound Healing Process: Evidence from In Vitro and In Vivo Experimental Models

Extraction optimization and antioxidant activity of Phyllanthus urinaria polysaccharides

Secondary Metabolites, Pharmacognostic and Therapeutic Activities of the Rhizome Extract of Curcuma longa Grown in South-West, Nigeria

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