Chemical Composition and Biological Activities of the Aqueous Fraction of Parkinsonea aculeata L. Growing in Saudi Arabia

Hаssаn, Wаfаа S.; Abdelaziz, Sahar; Yousef, Hanan M. Al

doi:10.1016/j.arabjc.2018.08.003

Cited by 62 publications

(50 citation statements)

References 39 publications

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“…curcas leaves. This flavonoid was also identified by ESI-MS in Parkinsonea aculeata m/z of 431 [M-H] − ( Hassan, Abdelaziz & Al Yousef, 2019 ). In this work it is also suggested the tentative identification of vicenin-2,6″- O -glucoside m/z 757 [M+H] + which has not been reported to Jatropha curcas , but to Stell aria holostea (Bouillant et al 1984) ( Fig.…”

Section: Discussionmentioning

confidence: 96%

Chemical analysis of callus extracts from toxic and non-toxic varieties of Jatropha curcas L.

Leyva-Padrón

Vanegas‐Espinoza

Evangelista‐Lozano

et al. 2020

PeerJ

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Jatropha curcas L. belongs to Euphorbiaceae family, and it synthesizes flavonoid and diterpene compounds that have showed antioxidant, anti-inflammatory, anticancer, antiviral, antimicrobial, antifungal and insecticide activity. Seeds of this plant accumulate phorbol esters, which are tigliane type diterpenes, reported as toxic and, depending on its concentration, toxic and non-toxic varieties has been identified. The aim of this work was to characterize the chemical profile of the extracts from seeds, leaves and callus of both varieties (toxic and non-toxic) of Jatropha curcas, to verify the presence of important compounds in dedifferentiated cells and consider the possibility of using these cultures for the massive production of metabolites. Callus induction was obtained using NAA (1.5 mg L−1) and BAP (1.5 mg L−1) after 21 d for both varieties. Thin layer chromatography analysis showed differences in compounds accumulation in callus from non-toxic variety throughout the time of culture, diterpenes showed an increase along the time, in contrast with flavonoids which decreased. Based on the results obtained through microQTOF-QII spectrometer it is suggested a higher accumulation of phorbol esters, derived from 12-deoxy-16-hydroxy-phorbol (m/z 365 [M+H]+), in callus of 38 d than those of 14 d culture, from both varieties. Unlike flavonoids accumulation, the MS chromatograms analysis allowed to suggest lower accumulation of flavonoids as the culture time progresses, in callus from both varieties. The presence of six glycosylated flavonoids is also suggested in leaf and callus extracts derived from both varieties (toxic and non-toxic), including: apigenin 6-C-α-L-arabinopyranosyl-8-C-β-D-xylopyranoside (m/z 535 [M+H]+), apigenin 4′-O-rhamnoside (m/z 417 [M+H]+), vitexin (m/z 433 [M+H]+), vitexin 4′-O-glucoside-2″-O-rhamnoside (m/z 741 [M+H]+), vicenin-2 (m/z 595 [M+H]+), and vicenin-2,6″-O-glucoside (m/z 757 [M+H]+).

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Section: Discussionmentioning

confidence: 96%

Chemical analysis of callus extracts from toxic and non-toxic varieties of Jatropha curcas L.

Leyva-Padrón

Vanegas‐Espinoza

Evangelista‐Lozano

et al. 2020

PeerJ

View full text Add to dashboard Cite

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“…The results obtained by microQTOF-QII of the molecular ion m/z 381 [M+H] + through the MS/MS experiment, and the predictive structures obtained through the CFM-ID platform allowed to suggest the relation of the structures from the molecular ion m/z 381 [M+H] + with the fragmentation profile from apigenin 6-C-α-L-arabinopyranosyl-8-C-β-D-xylopyranoside m/z 535 [M+H] + , which was identified through ESI-MS in Viola yedoensis (Xie et al, 2003) and apigenin 4'-O-rhamnoside m/z 417 [M+H] + , which was identified in Olea europaea (Pieroni et al, 1996). (Hassan, Abdelaziz & Al Yousef., 2019). In this work it is also suggested the tentative identification of vicenin-2,6''-O-glucoside m/z 757 [M+H] + which has not been reported to Jatropha curcas, but to Stellaria holostea (Bouillant et al, 1984) (Fig.…”

Section: Discussionmentioning

confidence: 99%

Peer Review #2 of "Chemical analysis of callus extracts from toxic and non-toxic varieties of Jatropha curcas L. (v0.2)"

2020

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“…The reverse-phase separations were performed (ACQUITY UPLC BEH C 18 Column, 1.7 μm–2.1 × 50 mm; 50 mm × 1.2 mm inner diameter; 1.7 μm particle size) at 0.2 m/mL flow rate. A previously reported gradient program was applied for the analysis ( Hassan et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, it has an incredible ability to survive and grow under variable environmental conditions and on different soils ( Van Klinken et al, 2009 ). Survey of the genus Parkinsonia revealed the presence of variable classes of compounds such as flavones, flavanones, flavone C -glycoside, alkaloids, sterols, tannins, terpenoids, glycerols, carotenoids, saponins, carbohydrates, free amino and fatty acids ( Hassan et al, 2019 , Sharma et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

“…However, dried seeds’ antimicrobial activity was due to the existence of L-dopa and trytophan amino acid ( Gupta et al, 2011 ). Few kinds of research were carried out on the aerial parts of P. aculeata L. growing in Saudi Arabia such as the antimicrobial activity of its essential oil and the chemical composition and biological activities of its aqueous fraction ( Al-Youssef and Hassan, 2015 , Hassan et al, 2019 ). Our previous results showed that aqueous fraction of P. aculeata L. growing in Saudi Arabia had forty tentatively identified compounds and showed a significant antioxidant activity.…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical profile, antioxidant and cytotoxic potential of Parkinsonia aculeata L. growing in Saudi Arabia

Abdelaziz

Yousef

Alqahtani

et al. 2020

Saudi Pharmaceutical Journal

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Parkinsonia aculeata L. growing in Saudi Arabia was investigated for its phytochemical profile, antioxidant, and cytotoxic properties. UPLC-ESI-MS/MS was employed as a powerful technique for the characterization of secondary metabolites from a hydroalcoholic extract, dichloromethane, and ethyl acetate fractions of P. aculeata L. aerial parts. Sixty-nine compounds (flavonoids, anthocyanins, phenolics and fatty acids) were detected and characterized; flavonoids were the abundant components in the analyzed samples. The dichloromethane fraction was rich in phenolics as vanillic acid hexoside, flavonols as 3,7-dimthylquercetin, and flavones as 3′-hydroxymelanettin. However, the ethyl acetate fraction was rich in flavonoid- C -glycosides as luteolin-8- C -β-D-glucoside (orientin) and apigenin-8- C -glucoside (vitexin), flavonoid- O , C -diglycosides such as luteolin 7- O -[6′'-dihydrogalloyl]-glucosyl-8- C -pentosyl-(1 → 2)-glucoside and 2′'- O -rhamnosyl isoorientin. These compounds were identified for the first time in dichloromethane and ethyl acetate fractions of Saudi P. aculeata L. Additionally, all the samples were assessed for antioxidant activity using DPPH radical scavenging method and for cytotoxic activity through MTT assay. Accordingly, the most active fraction was the ethyl acetate which showed the highest antioxidant activity (SC 50 = 57.4 ± 1.2 μg/mL) compared with the positive control, ascorbic acid (SC 50 = 12.4 ± 0.5 μg/mL) and moderate cytotoxicity against HepG-2 (hepatocellular carcinoma) and MCF-7 (breast carcinoma) cell lines with IC 50 = 56.9 ± 3.1 and 95.8 ± 3.8 μg/mL, respectively compared with cisplatin (IC 50 = 3.67 ± 0.22 and 5.71 ± 0.57 μg/mL, respectively for both cell lines). The antioxidant and cytotoxic activities may be attributed to the presence of high percentage of phenolic compounds and hydroxylated flavonoids detected in ethyl acetate fraction using UPLS-ESI-MS/MS.

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Chemical Composition and Biological Activities of the Aqueous Fraction of Parkinsonea aculeata L. Growing in Saudi Arabia

Cited by 62 publications

References 39 publications

Chemical analysis of callus extracts from toxic and non-toxic varieties of Jatropha curcas L.

Chemical analysis of callus extracts from toxic and non-toxic varieties of Jatropha curcas L.

Peer Review #2 of "Chemical analysis of callus extracts from toxic and non-toxic varieties of Jatropha curcas L. (v0.2)"

Phytochemical profile, antioxidant and cytotoxic potential of Parkinsonia aculeata L. growing in Saudi Arabia

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