Chemical composition and chromatographic fingerprint of three strains of Agaricus subrufescens cultivated with handmade and commercial supplements

Ferrari, Anna Beatriz Sabino; Marcheafave, Gustavo Galo; Mannochio-Russo, Helena; Bolzani, Vanderlan da Silva; Zied, Diego Cunha; Scarmínio, Ieda Spacino; Zeraik, Maria Luiza

doi:10.1016/j.foodchem.2021.130227

Cited by 5 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Each 0.5 g of fresh mycelium from the WT, PoNoxA OE , PoNoxA RNAi , PoNoxB OE , and PoNoxB RNAi transformants was ground into powder using liquid nitrogen in separate polyethylene tubes. Samples were preparated for the mass spectrometry assay as previously reported (Sabino Ferrari et al, 2021 ). An Ultra Performance Liquid Chromatography (UPLC) system Acquity I-Class PLUS (Waters, USA) coupled with the mass spectrometer Xevo G2-XS QTOF (Waters, USA) was used to analyze the metabolites (Sabino Ferrari et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…Samples were preparated for the mass spectrometry assay as previously reported (Sabino Ferrari et al, 2021 ). An Ultra Performance Liquid Chromatography (UPLC) system Acquity I-Class PLUS (Waters, USA) coupled with the mass spectrometer Xevo G2-XS QTOF (Waters, USA) was used to analyze the metabolites (Sabino Ferrari et al, 2021 ). All measurements were replicated six times.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Contrasting effects of NADPH oxidases on the fungal hyphae growth and immune responses in Pleurotus ostreatus

Li,

Zhu,

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Pleurotus ostreatus is one of the most consumed mushroom species, as it serves as a high-quality food, favors a rich secondary metabolism, and has remarkable adaptability to the environment and predators. In this study, we investigated the function of two key reactive oxygen species producing enzyme NADPH oxidase (PoNoxA and PoNoxB) in P. ostreatus hyphae growth, metabolite production, signaling pathway activation, and immune responses to different stresses. Characterization of the Nox mutants showed that PoNoxB played an important role in the hyphal formation of the multicellular structure, while PoNoxA regulated apical dominance. The ability of P. ostreatus to tolerate a series of abiotic stress conditions (e.g., osmotic, oxidative, membrane, and cell-wall stresses) and mechanical damage repair was enhanced with PoNoxA over-expression. PoNoxB had a greater responsibility in regulating the polysaccharide composition of the cell wall and methyl jasmonate and gibberellin GA1 biosynthesis, and improved mushroom resistance against Tyrophagus putrescentiae. Moreover, mutants were involved in the jasmonate and GA signaling pathway, and toxic protein defense metabolite production. Our findings shed light on how the oyster mushroom senses stress signals and responds to adverse environments by the complex regulators of Noxs.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Contrasting effects of NADPH oxidases on the fungal hyphae growth and immune responses in Pleurotus ostreatus

Li,

Zhu,

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Each 0.5 g of fresh mycelium from the WT, PoNoxA OE , PoNoxA RNAi , PoNoxB OE , and PoNoxB RNAi transformants was ground into powder using liquid nitrogen in separate polyethylene tubes. Samples were preparated for the mass spectrometry assay as previously reported (Sabino Ferrari et al, 2021). An Ultra Performance Liquid Chromatography (UPLC) system Acquity I-Class PLUS (Waters, USA) coupled with the mass spectrometer Xevo G2-XS QTOF (Waters, USA) was used to analyze the metabolites (Sabino Ferrari et al, 2021).…”

Section: Uplc-ms-based Metabolomicsmentioning

confidence: 99%

“…Samples were preparated for the mass spectrometry assay as previously reported (Sabino Ferrari et al, 2021). An Ultra Performance Liquid Chromatography (UPLC) system Acquity I-Class PLUS (Waters, USA) coupled with the mass spectrometer Xevo G2-XS QTOF (Waters, USA) was used to analyze the metabolites (Sabino Ferrari et al, 2021). All measurements were replicated six times.…”

Section: Uplc-ms-based Metabolomicsmentioning

confidence: 99%