Chemical Composition and Evaluation Preliminary of Antileishmanial Activity<i>in vitro</i>of Essential Oil from Leaves of<i>Eugenia pitanga</i>, A Native Species of Southern of Brazil

Kauffmann, Carla; Ethur, Eduardo Miranda; Arossi, Kelen; Hoehne, Lucélia; Freitas, Elisete Maria de; Machado, Gérzia Maria de Carvalho; Cavalheiro, Marilene Marcuzzo do Canto; Flach, Adriana; Costa, Luiz Antonio Mendonça Alves da; Gnoatto, Simone Cristina Baggio

doi:10.1080/0972060x.2017.1281767

Cited by 10 publications

(3 citation statements)

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“…Coumarin, its various analogues and derivatives are reported to be antileishmanial [57, 58], antitrypanosomal [59], antifungal [60], anticancer [61] and antimicrobial [62]. Viridiflorol, another plant secondary metabolite present in CBD, has also been detected in other medicinal plant fractions or extracts that were reported to be antileishmanial [63], antifungal [64] and antibacterial [65].…”

Section: Discussionmentioning

confidence: 99%

Cinnamomum cassia exhibits antileishmanial activity against Leishmania donovani infection in vitro and in vivo

et al. 2019

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Background There is a pressing need for drug discovery against visceral leishmaniasis, a life-threatening protozoal infection, as the available chemotherapy is antiquated and not bereft of side effects. Plants as alternate drug resources has rewarded mankind in the past and aimed in this direction, we investigated the antileishmanial potential of Cinnamomum cassia . Methodology Dichloromethane, ethanolic and aqueous fractions of C . cassia bark, prepared by sequential extraction, were appraised for their anti-promastigote activity along with apoptosis-inducing potential. The most potent, C . cassia dichloromethane fraction (CBD) was evaluated for anti-amastigote efficacy in infected macrophages and nitric oxide (NO) production studied. The in vivo antileishmanial efficacy was assessed in L . donovani infected BALB/c mice and hamsters and various correlates of host protective immunity ascertained. Toxicity profile of CBD was investigated in vitro against peritoneal macrophages and in vivo via alterations in liver and kidney functions. The plant secondary metabolites present in CBD were identified by gas chromatography-mass spectroscopy (GC-MS). Principal findings CBD displayed significant anti-promastigote activity with 50% inhibitory concentration (IC 50 ) of 33.6 μg ml -1 that was mediated via apoptosis. This was evidenced by mitochondrial membrane depolarization, increased proportion of cells in sub-G 0 -G 1 phase, ROS production, PS externalization and DNA fragmentation (TUNEL assay). CBD also inhibited intracellular amastigote proliferation (IC 50 14.06 μg ml -1 ) independent of NO production. The in vivo protection achieved was 80.91% (liver) and 82.92% (spleen) in mice and 75.61% (liver) and 78.93% (spleen) in hamsters indicating its profound therapeutic efficacy. CBD exhibited direct antileishmanial activity, as it did not specifically induce a T helper type (Th)-1-polarized mileu in cured hosts. This was evidenced by insignificant modulation of NO production, lymphoproliferation, DTH (delayed type hypersensitivity), serum IgG2a and IgG1 levels and production of Th2 cytokines (IL-4 and IL-10) along with restoration of pro-inflammatory Th1 cytokines (INF-γ, IL-12p70) to the normal range. CBD was devoid of any toxicity in vitro as well as in vivo . The chemical constituents, cinnamaldehyde and its derivatives present in CBD may have imparted the observed antileishmanial effect. Conclusions Our study hi...

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Section: Discussionmentioning

confidence: 99%

Cinnamomum cassia exhibits antileishmanial activity against Leishmania donovani infection in vitro and in vivo

et al. 2019

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“…showed in vitro leishmanicidal activity against promastigote forms of Leishmania amazonenses (KAUFFMANN et al, 2017a). The essential oil of E. pitanga also showed action against L. amazonenses, with an inhibitory potential similar to that of the standard drug pentamidine (KAUFFMANN et al, 2017b). Another study with extracts of Eugenia anomala D.Legrand.…”

Section: Why Preserve the Southernmost Fields Of Brazil?mentioning

confidence: 78%

Why preserve the southernmost fields of Brazil?

Freitas

Silva

Liesenfeld

et al. 2020

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In Brazil, grassland ecosystems of the Pampa are restricted to Rio Grande do Sul State (RS) and constitute the largest temperate natural grassland in the world. There are no recent data to indicate how much of these fields was lost in the state, but excursions made in recent years have pointed to a high conversion of native fields into crops. This situation has alerted to the intensification of biodiversity loss and the biological impoverishment of these fields. In view of the above, this study characterizes plant diversity in fields of the southern and western portion of RS. The study was based on publications of recent years that indicate the problems that effectively threaten this diversity. Pampa fields are natural and have a great diversity, constituting a considerable genetic heritage, ensuring the provision of ecosystem services to human populations. The few bioprospecting studies on species from these fields suggest that much remains to be discovered and that actions need to be taken to inhibit field destruction. These fields have never been treated as priority areas for conservation, but it is our commitment to reverse this transformation and destruction.

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“…100 μg/mL is inactive (Andrade et al, 2018). According to Kauffmann et al (2017), the observed antileishmanial activity may be attributed to the mixture of sesquiterpene constituents. The promising leishmanicidal activity exhibited by EO from M. paniculata leaves and ripe fruits can be attributed to the chemical constituent trans-βcaryophyllene, which was identified at high concentration in EO under study.…”

Section: Antileishmanial Activity Of Eo From M Paniculata Leaves and Fruits (Ripe And Unripe Ones)mentioning

confidence: 97%

In vitro antileishmanial and antioxidant activities of essential oils from different parts of Murraya paniculata (L.) Jack: a species of Rutaceae that occur in the Cerrado biome in Brazil

et al. 2020

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In Brazilian folk medicine, Murraya paniculata has been used for treating intestine disorders, rheumatism and cough. This paper aims to investigate the in vitro antileishmanial and antioxidant activities of essential oils (EO) from M. paniculata leaves and fruits (ripe and unripe ones). Natural antioxidants may be very beneficial to improve quality of life, since they are capable of protecting the body against damage caused by free radicals and, consequently, either preventing or postponing many diseases from starting their cycles. One of the techniques which has been widely used for detecting antioxidant compounds is the method based on the elimination of the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•). It has been considered easy, precise, fast, simple, economical and appropriate to determine antioxidant activity of pure substances and complex mixtures, such as EO. Thus, antioxidant potential of EO was evaluated by using the method of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•). EO from M. paniculata leaves and fruits exhibited weak potential, since EC50 values were above 700 µg/mL. Several reports have stated that volatile oils from plants show promising leishmanicidal activity against promastigote forms of Leishmania amazonensis; in relation to this activity, leaf oil was highly active (IC50 = 7.33±2.07 µg/mL) while ripe and unripe fruit oils were active, with values of IC50 = 30.77±2.07 µg/mL and 13.04±1.64 µg/mL, respectively. Both GC-FID and GC-MS analyses revealed that the major components determined in EO from M. paniculata were sesquithujene (25.0%), trans-β-caryophyllene (23.8%), α-zingiberene (21.0%), α-ylangene (13.3%), germacrene D (13.1%), α-copaene (12.7%), and β-cubebene (10.2%). In vitro antileishmanial and antioxidant activities of EO from M. paniculata have also been described for the first time.

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Chemical Composition and Evaluation Preliminary of Antileishmanial Activityin vitroof Essential Oil from Leaves ofEugenia pitanga, A Native Species of Southern of Brazil

Cited by 10 publications

References 31 publications

Cinnamomum cassia exhibits antileishmanial activity against Leishmania donovani infection in vitro and in vivo

Cinnamomum cassia exhibits antileishmanial activity against Leishmania donovani infection in vitro and in vivo

Why preserve the southernmost fields of Brazil?

In vitro antileishmanial and antioxidant activities of essential oils from different parts of Murraya paniculata (L.) Jack: a species of Rutaceae that occur in the Cerrado biome in Brazil

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