Chemical composition and hepatoprotective activity of ethanolic root extract of Taraxacum Syriacum Boiss against acetaminophen intoxication in rats

Abstract

Abstract:Aim: In the present study, the role of ethanol extract of root of Taraxacum Syriacum Boiss (TSBE) against hepatotoxicity caused by acetaminophen (APAP) was studied. Methods: The chemical composition of roots of Taraxacum Syriacum Boiss was analyzed by SPME-GC/MS method. Hepatocellular injuries induced by acetaminophen (APAP) were assessed by liver histology, serum aminotransferase activities, antioxidant enzymes activity and lipid peroxidation in liver tissue. Results: TSBE was observed to exhibit hepatoprotective effect as demonstrated by signifi cant decrease in serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), and alkaline phosphatase (ALP) concentration, and by preventing liver histopathologic changes in rats with APAP hepatotoxicity. Administration of APAP, signifi cantly increased, lactate dehydrogenase (LDH) and catalase (CAT) activity in liver tissue and pretreatment with TSBE returned these parameters to control group, moreover TSBE reduces APAP-induced hepatic Glutathione (GSH) depletion. Carvacrol (6.7 %) was the main polyphenolic compound of plant sample. Our results demonstrated hepatoprotective activity of TSBE in rat in vivo. Conclusions: We believe that the mechanism by which the extract was able to protect the liver from the oxidative stress generated by APAP is due to its antioxidant activity. These phenolic compounds of the extract act as antioxidants and free radical scavengers and reduce or inhibit the oxidative stress induced by APAP administration (Tab. 3, Fig. 3, Ref. 39). Text in PDF www.elis.sk.

Protective Effects of Combined Selenium and Punica granatum Treatment on Some Inflammatory and Oxidative Stress Markers in Arsenic-Induced Hepatotoxicity in Rats

“…Anti-oxidant treatment had been widely used in the treatment of the noxious effects of heavy metal toxicity [8]. In the present study, the protective role of combined selenium and P. granatum against liver injury induced by arsenic in rats had been studied by determining their effects on some oxidative stress and inflammatory markers in addition to Nrf2 gene expression.…”

“…As one of the enzymes regulated by Nrf2 transcription factor, thioredoxin reductase (TrxR) is a cellular antioxidant containing selenocysteine in its C-terminal redox center, resulting in increase in total anti-oxidant capacity [7]. Growing body of evidences highlights the anti-oxidant ameliorative role on the free radical generation that occurs in heavy metal toxicity [8]. Selenium, an essential dietary trace element for mammals, is a cofactor of glutathione peroxidase (GSH-Px), an anti-oxidant enzyme that protects lipids and macromolecules in the liver cell intracellular and cellular membranes against oxidative damage generated by reactive oxygen species (ROS) [9,10].…”

Evaluation of Hepatoprotective activity of Eriocaulon quinquangulare in vitro using porcine liver slices against ethanol induced liver toxicity and free radical scavenging capacity

“…The plant extracts alone in KRHB did not show any toxicity on liver tissue over the concentrations we studied. In a recent study carried out by Nazari and coauthors (2015), it has been found that ethanolic root extract of Taraxacum Syriacum Boiss can exert hepatoprotective activity against liver toxicity induced by acetaminophen (APAP) intoxication in rats [ 34 ]. The authors believe that the mechanism through which the extract protects the liver from the oxidative stress caused by APAP is due to its antioxidant activity.…”

Panorama atual do modelo de indução da hepatotoxicidade por paracetamol para estudos de hepatoproteção em ratos: Scoping review / Current overview of the model of induction of hepatotoxicity by paracetamol for studies of hepatoprotection in rats: Scoping review

“…Embora existam diversas vias de administração, nos trabalhos selecionados para esta revisão a via oral e intraperitoneal foram as únicas citadas. Dos estudos, 18 utilizaram a via peritoneal (NAZARI et al,2015;MEHRA et al, 2016;ELBE et al, 2018). Essa via de administração é fácil de realizar em animais de laboratório e garante uma maior absorção que a via oral.…”