Chemical composition and larvicidal activity against Aedes mosquitoes of essential oils from Arisaema fargesii

Abstract

BACKGROUND: Dengue fever is caused by the spread of dengue virus by Aedes mosquito vectors. Currently, the most effective way to control dengue is by preventing mosquitoes from spreading the disease. Arisaema fargesii is a Chinese herbal medicine commonly used to repel mosquitoes. In our laboratory, anti-mosquito chemical components were extracted from A. fargesii, and the effects of these substances on mosquito larvae were examined. RESULTS: In total, 48 compounds corresponding to 98.79% of the total oil were identified and the major compounds identified were linalool (12.38%), carvacrol (8.27%), eugenol (5.21%), and -selinene (5.36%). Essential oil had larvicidal activity against Ae. aegypti and Ae. albopictus with LC 50 values of 40.49 mg/L, 47.01 mg/L, respectively. The LC 50 values of carvacrol, eugenol, linalool and -selinene were 32.78, 56.34, 70.56, 136.03 mg/L against Ae. aegypti larvae, and 39.08, 52.07, 82.34, 151.74 mg/L, respectively, against Ae. albopictus larvae. Biochemical assays of Aedes larvae showed that the activities of acetylcholinesterase (AChE), monooxygenases (MO), glutathione-S-transferase (GST), p-Nitrophenyl acetate (p-NPA) esterase, -esterase and -esterase were significantly affected by carvacrol. Essential oil induced the detoxification mechanism for the action of GST and MO.CONCLUSION: The result indicates that essential oil of A. fargesii and its isolated constituent have good inhibitory effects on the defense enzymes of Aedes mosquito larvae. A. fargesii essential oil can be used to control Aedes mosquito larvae to prevent the spread of dengue fever. /journal/ps a Retention index relative to n-alkanes on HP-5 MS capillary column. b Relative area (peak area relative to the total peak area). c RI, retention index, MS, mass spectrum, Co, co-injection with authentic compound. Pest Manag Sci 2020; 76: 534-542

Acaricidal activity of Cinnamomum cassia (Chinese cinnamon) against the tick Haemaphysalis longicornis is linked to its content of (E)-cinnamaldehyde

“…cassia essential oil and ( E )-cinnamaldehyde. Esterase and monooxygenase are involved in the metabolic detoxification of pesticides [ 33 ]. In ticks, they play an important role in the development of resistance to synthetic acaricides [ 18 , 34 ].…”

“…cassia essential oil and ( E )-cinnamaldehyde. However, additional studies are needed to test different times of exposure, since esterase and monooxygenase activities may decrease with time [ 33 ], and to explore the contribution of other metabolic enzymes such as acetylcholinesterase and glutathione S-transferases to defense mechanisms. It is important to note that the total protein content of H .…”

Essential oil of Piper purusanum C.DC (Piperaceae) and its main sesquiterpenes: biodefensives against malaria and dengue vectors, without lethal effect on non-target aquatic fauna

“…Furthermore, the larvicidal activity of β-caryophyllene, α-humulene and germacrene D corroborates other larvicidal studies (Ravi et al 2006;Govindarajan and Benelli 2016a, b). Although caryophyllene oxide, α-terpineol, β-selinene and E-nerolidol were not investigated in our study, they are important larvicidal agents (Cheng et al 2004;Pavela 2015;Huang et al 2019), and could contribute through a synergic effect to the improvement of the larvicidal activity.…”

Chemical Composition, Larvicidal Activity, and Enzyme Inhibition of the Essential Oil of Lippia grata Schauer from the Caatinga Biome against Dengue Vectors

“…aegypti and Ae. albopictus larvae [65]. Studies of different species have shown that thymol promoted toxicity in Plutella xylostella larvae, causing an increase in esterases levels, whereas in Ephestia kuehniella Zeller, inhibition of these enzymes was observed [66,67].…”