Chemical composition and pharmacological activities of Pisum sativum

Abstract

BackgroundConsumption of vegetables has been proven to be effective in the prevention of different diseases. Traditionally edible aerial part of Pisum sativum L. subsp. sativum (Fabaceae) is used to treat diabetes, heart diseases and as blood purifier. Present study was aimed to explore the traditional use of aerial parts of P. sativum as a source of antidiabetic agent. In addition, antioxidant activity and chemical composition was carried out.MethodsTotal polyphenol content was spectrophotometrically determined using Folin Chiocalteu’s reagent while the flavonoids by aluminum chloride colorimetric assay. Identification of compounds of the extract was made through HPLC and LCMS. Antihyperglycemic activity was assessed by oral glucose tolerance test in mice. Antioxidant activity was determined by DPPH free radical scavenging and reducing power assay.ResultsTotal polyphenol and total flavonoids content were found to be 51.23 mg gallic acid equivalent and 30.88 mg quercetin equivalent per gram of dried plant extract respectively. Ellagic acid and p-coumeric acid were detected through HPLC. A total of eight compounds including naringenin, β-sitosterol were indentified through LCMS. In OGTT, extract (200 mg/kg bw) showed a 30.24% decrease (P< 0.05) in blood glucose levels at 30 min compared to the normal control. The extract showed IC50 value of 158.52 μg/mL in DPPH scavenging assay and also showed comparable reducing power.ConclusionAlong with other compounds ellagic acid and β-sitosterol present in the extract may be responsible for its antioxidant as well as antihyperglycemic activities. Altogether these results rationalize the use of this vegetable in traditional medicine.

Understanding glycaemic control and current approaches for screening antidiabetic natural products from evidence-based medicinal plants

“…In a study by Zilani [274] the authors used HPLC and LC–MS which lead to the detection of ellagic acid, naringenin, β-sitosterol, and p-coumaric acid in a Pisum sativum (peas) extract which also exhibited in vitro antioxidant properties, and improved oral glucose tolerance in diabetic mice. Sixteen compounds including caffeic acid, luteolin glucoside, xanthomicrol, carvacrol, and luteolin were putatively identified from an Origanum glandulosum extract via LC–MS.…”

Multiple pharmacological approaches on Fibigia eriocarpa extracts by in vitro and computational assays

“…The therapeutic uses of phenolic compounds have attracted much interest as they exert a wide range of bioactivities, including antioxidant [38][39][40], antibacterial [41][42][43], antifungal [43][44][45], anti-inflammatory [46,47], and anticancer [48,49] activities. Therefore, the phenolic content can be used as an important indicator of biological attributes of plant extracts.…”

In-vitro antioxidant, anti-cancer, and anti-inflammatory activities of selected medicinal plants from western Nepal

“…Further, plant-based medication are the basis of the modern pharmaceuticals and search for novel and functional extracts from medicinal plants are in central attraction in recent years because of the presence of diverse bioactive secondary metabolites such as alkaloids, terpenoids, glycosides, steroids, flavonoids, and phenolic compounds [5]. These bioactive molecules are reported to have several beneficial effects in lowering the risk of diseases caused by reactive oxygen species (ROS) [6] through different mechanisms of action such as scavenging free radicals, quenching ROS, inhibiting oxidative enzymes [7]. It is evident that plant extracts have diverse biochemical activities such as anti-allergic, antiinflammatory, antioxidant, anti-microbial, anti-fungal, antiviral, and anti-cancer [7,8].…”