Chemical composition and solubility of human glomerular and tubular basement membranes of adult and senescent men

Langeveld, J.P.M.; Veerkamp, J.H.; Trubels, J.M.F.; duyf, carla M.P.; Monnens, L

doi:10.1016/0020-711x(84)90225-8

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…These include: (1) progressive decline in the number of intact or normal glomeruli with age [142], (2) increase in the number/percentage of globally sclerotic glomeruli, especially those of the outer cortical regions initially [143][144][145][146][147][148], (3) abnormal glomeruli with shunts between the afferent and efferent arterioles bypassing the (especially juxtamedullary) glomeruli [149], (4) progressive decrease, and then later increase, in the size of intact glomeruli (especially the juxtamedullary glomeruli) [150,151], (5) Focal or diffuse thickening of the glomerular basement membranes [152][153], (6) increased mesangial volume/matrix-sclerosis [132-134, 138, 140, 141], and recently, (7) decreased number of glomeruli related directly to birth weight which would predispose the kidney to the condition(s) of aging [154]. Often the increase in mesangial matrix directly correlates with the GBM thickening (although not necessarily with proteinuria).…”

Section: Structurementioning

confidence: 99%

The Aging Kidney: A Review – Part I

Silva

2005

Int Urol Nephrol

100

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Section: Structurementioning

confidence: 99%

The Aging Kidney: A Review – Part I

Silva

2005

Int Urol Nephrol

100

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“…It amounted to at least 90%, in general to 95-100% except for the glomerular preparations of child ren up to 1 year old, where the purity was 70-90%. The basement membrane preparations were characterized morphologically and by chemical analysis of the carbo hydrate and amino acid composition [10,12]. Alkali-solu bilized whole basement membranes contained 66 ±4% protein (mean ±SD, percentage of dry weight, 29 prepar ations).…”

Section: Resultsmentioning

confidence: 99%

“…Previously we described age-related dif ferences in the amino acid and carbohydrate composi tion of bovine and human renal basement membranes [10][11][12]. Since glycosaminoglycans may contribute more to the charge-selective characteristics of the basement membranes, we examined in this study the glycosamino glycan content of glomerular and tubular basement mem branes in 38 and 34 preparations, respectively, from indi viduals ranging in age from preterm neonates to 90 years.…”

Section: Introductionmentioning

confidence: 99%

Sulfated Glycosaminoglycan Content of Glomerular and Tubular Basement Membranes of Individuals of Different Ages

Reubsaet¹,

Veerkamp²,

Monnens

1985

Nephron

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The glycosaminoglycan content of glomerular and tubular basement membranes in individuals ranging from preterm neonates to 90-years-old people was determined with a spectrophotometric assay after papain digestion. A decrease of glycosaminoglycans until the age of 2.5 years was observed only in glomerular basement membrane. Tubular basement membranes did not show significant changes with age. Glomerular basement membranes of neonates had a higher glycosaminoglycan content than tubular basement membranes.

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Effect of age and diabetes on type IV collagen and laminin in human kidney cortex

Karttunen

Risteli

Autio–Harmainen

et al. 1986

Kidney International

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Specific radioimmunoassays for the 7-S domain of type IV collagen and the fragment P1 of laminin were used to quantify these basement membrane proteins in human kidney cortex at different ages and in some patients with diabetes mellitus. The antigens were solubilized by treating the tissue samples with the proteolytic enzymes collagenase, trypsin and pepsin. Total collagen content (as indicated by hydroxyproline concentration) increased with age, and the proportion of the collagen that could be solubilized by any enzyme treatment decreased. The type IV collagen concentration increased significantly with age, whereas the laminin concentration tended to decrease. In the one case of a type I diabetic the amounts of both antigens exceeded those in the age matched controls. In four type II diabetics the results were comparable with those for other aged cases. The distribution of the proteins was studied using the peroxidase-antiperoxidase method. The staining intensity and thickness of both antigens increased with age in the mesangium and Bowmans capsules, the change in type IV collagen staining being more evident. In diabetic patients these changes were more pronounced and other basement membranes appeared thicker in the stainings. These results indicate that basement membrane material accumulates in the kidney cortex during aging and that an alteration takes place in the composition of the basement membranes, the proportion of type IV collagen increasing and that of laminin decreasing.

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Chemical composition and solubility of human glomerular and tubular basement membranes of adult and senescent men

Cited by 5 publications

References 33 publications

The Aging Kidney: A Review – Part I

The Aging Kidney: A Review – Part I

Sulfated Glycosaminoglycan Content of Glomerular and Tubular Basement Membranes of Individuals of Different Ages

Effect of age and diabetes on type IV collagen and laminin in human kidney cortex

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