Chemical Composition, Antibacterial, Antioxidant and Enzyme Inhibitory Activities of the Essential Oil from Leaves of Psidium guajava L.

Abstract

Guava (Psidium guajava L.) leaf essential oil (GLEO) was extracted by water distillation, and its in vitro antioxidant, antidiabetic, and antibacterial properties were evaluated. Using GC/MS to determine the chemical components of GLEO, 27 constituents were identified, accounting for 74.90 % of the total oil content, among which L-caryophyllene (24.46 %), L-calamenene (10.82 %), (À )-globulol (10.69 %), and α-copaene (8.71 %) were the main components. Subsequently, the antioxidant activity of GLEO was determined by DPPH, ABTS, and βcarotene bleaching tests. The half maximal inhibitory concentration of GLEO for three free radicals were IC 50 = 17.66 � 0.07 μg/mL, IC 50 = 19.28 � 0.03 μg/mL, and IC 50 = 3.17 � 0.01 μg/mL, respectively. Moreover, GLEO exhibited remarkable α-amylase (IC 50 = 13.99 � 0.34 μg/mL) and α-glucosidase (IC 50 = 5.50 � 1.02 μg/mL) inhibitory activities. It was effective against Streptomyces acidiscabies (MIC = 1.25 μg/mL), Ralstonia solanacearum (MIC = 5 μg/mL), and Erwinia carotovora subsp carotovora borgey (MIC = 2.5 μg/mL), showing significant antibacterial properties. Based on the findings, given the high biological activity of GLEO, it is a biological preservative for food, medicine, and cosmetics and is valuable in natural therapy and crop disease management.

Guava Leaf Essential Oil as a Potent Antioxidant and Anticancer Agent: Validated through Experimental and Computational Study

“…The primary content of monoterpenes like limonene and eucalyptol act as a radical scavenger through an electron or hydrogen donating mechanism and the potential for antioxidant activity was shown to be closely correlated with the antioxidant activity previously reported [ 44 ]. As part of comparative study, we found results similar to those obtained by Zang et al (IC 50 of 17.66 ± 0.07 µg/mL) [ 45 ].…”

Extraction from different parts of Citrus maxima flowers using ultrasound as an aid and study of their composition and function

“…It was confirmed that the FCM's various parts possessed considerable antioxidant properties. FCM recorded a better free radical scavenging effect when compared to the anti-free radical previously reported [18] . The ethanol as an extraction solvent had a somewhat stronger antioxidant effect than hot water, according to the aforementioned trials.…”

“…At 734 nm, absorbance was observed. Positive control was provided by Vc with the same gradient of concentration [18] . Three parallel tests of the aforementioned stages were conducted, with the average result.…”

Ethnobotanical Review of Selected Medicinal Plants in Guam for the Treatment of Urinary Tract Ailments and Their Pharmacological Properties

“…The half-maximal inhibitory concentration of guava leaves for DPPH, ABTS, and β-carotene bleaching tests are IC50 = 17.66 ± 0.07 µg/mL, IC50 = 19.28 ± 0.03 µg/mL, and IC50 = 3.17 ± 0.01 µg/mL, respectively [38]. The leaf extracts have also shown significant antibacterial activity against Streptomyces acidiscabies (MIC = 1.25 µg/mL), Ralstonia solanacearum (MIC = 5 µg/mL), and Erwinia carotovora subsp carotovora borgey (MIC = 2.5 µg/mL).…”

“…Leaves: antidiabetic [35,36]; antioxidant [37,38]; anti diarrhoeal [39,40]; antibacterial [38,41]; anticancer [42,43].…”