Chemical composition, antileishmanial, and cytotoxic effects Ferula macrecolea essential oil against Leishmania tropica

Mahmoudvand, Hossein; Yadegari, Javad Ghasemian; Khalaf, Amal Khudair; Hashemi, Mohammad Javad; Dastyarhaghighi, Setareh; Salimikia, Iraj

doi:10.1016/j.parepi.2022.e00270

Cited by 7 publications

(13 citation statements)

References 32 publications

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“…Ferula macrecolea essential oil at 150 and 300 μg/mL showed good antiparasitic effects against Echinococcus granulosus protoscoleces (21). In another study, the effects of this essential oil and terpinolene displayed a strong antiparasitic effect on Leishmania tropica (22). The results of this study are consistent with the results of our study.…”

Section: Discussionsupporting

confidence: 92%

“…The CC 50 values of terpinolene against HGF1-PI1 and HepG2 cells were 279.6 and 108.4 μg/mL, respectively, indicating the essential oil and terpinolene were safe for normal cells and toxic for HepG2 cancer cells. Similarly, Mahmoudvand et al have reported the CC 50 of F. macrecolea essential oil and terpinolene on J774-A1 macrophage cells as 471.3 and 207.3 μg/mL, respectively (22).…”

Section: Discussionmentioning

confidence: 76%

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Chemical composition, anti-fungal and cytotoxic effects of Ferula macrecolea essential oil against Candida albicans resistant and sensitive strains

Sadeghi¹,

Sadeghi²,

Mohan³

et al. 2023

J Herbmed Pharmacol

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Introduction: Candidiasis therapy is a complicated concern because of the occurrence of resistance to antifungal agents. We studied the anti-fungal effects of Ferula macrecolea essential oil (FME) against Candida albicans resistant and sensitive strains, as well as its cytotoxic effects against normal and cancer cell lines. Methods: The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of F. macrecolea essential oil against C. albicans ATCC 5027 and C. albicans ATCC 76616 were studied by broth-microdilution approach. The cytotoxicities of FME on HGF1-PI (normal gingival cell line) and HepG2 (liver cancer cell line) cells were also studied. Results: The main components of essential oil were terpinolene (71.25%), n-nonanal (6.32%), and linalool (3.95%), respectively. The MIC and MFC of FME on C. albicans sensitive to nystatin were 1.6 and 2.0 μg/mL, respectively. The MIC and MFC of FME on nystatin-resistant strains were 3.3 and 4 μg/mL, respectively. The MIC and MFC of terpinolene on C. albicans sensitive to nystatin were 0.8 and 1.0 μg/mL, respectively. The MIC and MFC of terpinolene on nystatin-resistant strains were 2 and 2.4 μg/mL, respectively. The essential oil and terpinolene had no significant cytotoxic effects against normal cells. Conclusion: We revealed the promising antifungal effect of F. macrecolea essential oil and its main component, terpinolene, against C. albicans sensitive and resistant to nystatin with no significant toxicity on normal cells.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 76%

Chemical composition, anti-fungal and cytotoxic effects of Ferula macrecolea essential oil against Candida albicans resistant and sensitive strains

Sadeghi¹,

Sadeghi²,

Mohan³

et al. 2023

J Herbmed Pharmacol

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show abstract

“…Also, the viability of parasites significantly decreased in both growth phases using all drug concentrations compared to the control ( 43 ). Moreover, Mahmoudvand et al found the presence of myrtenal, linalool, terpinolene, terpinen-4-ol, and β-phellandrene in the essential oil of F. macrecolea and reported its great antileishmanial effects in vitro ( 13 ). Besides, Andrade et al reported the slight effects of F. galbaniflua essential oil on L. amazonensis promastigotes and brine shrimp (IC 50 = 95.70 ± 1.82 µg/mL and CC 50 = 377.26 ± 2.71 µg/mL, respectively) ( 44 ).…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, 50% cytotoxicity concentrations (CC 50 ) of the extracts and fractions were determined as a 50% reduction in the cell viability of treated cells relative to untreated cells. The ratio of CC 50 for macrophage cells to IC 50 for amastigotes was defined as the selectivity index (SI) ( 13 , 19 ).…”

Section: Methodsmentioning

confidence: 99%

“…Some metabolites and extracts from some Ferula species have been identified to have pharmaceutical and biological activities, such as antifungal ( 8 ), antibacterial ( 9 , 10 ), and antiviral ( 11 ) effects. Several studies have shown that Ferula species have potent antiprotozoal effects against Leishmania major ( 12 ), Leishmania tropica ( 13 ), Plasmodium falciparum ( 14 ), Trichomonas vaginalis ( 15 ), and Echinococcus granulosus ( 16 ).…”

Section: Introductionmentioning

confidence: 99%

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In vitro Evaluation of the Potent Antileishmanial Activity of Ferula tabasensis Alone or in Combination with Shark Cartilage Extract Against the Standard Iranian Strain of Leishmania major (MRHO/IR/75/ER)

Saraei,

Soozangar,

Miran

et al. 2023

Iran J Pharm Res

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Background: The available drugs for the treatment of leishmaniasis are highly toxic and extremely expensive, with low efficiency; therefore, the development of effective therapeutic compounds is essential. Objectives: The present study aimed to explore the antileishmanial effects of ethyl acetate extract, methanol extract, and fractions 1-4 (F1-F4) of Ferula tabasensis, alone or in combination with shark cartilage extract (ShCE), on L. major in vitro. Methods: In this study, ethyl acetate, methanol, and n-hexane extracts were extracted from the aerial roots of F. tabasensis by the maceration method. The silica gel column chromatography was used to separate n-hexane extracts at varying polarities (F1-F4 fractions). Subsequently, the effects of extracts and fractions against promastigotes were assessed by the parasite counting method microscopic inhibition test and MTT assay. Besides, their effects on the infected macrophage cells and the number of amastigotes were investigated. Cytotoxicity was evaluated in non-infected J774A.1 macrophage cells. Finally, apoptosis induction of promastigotes, including infected and non-infected macrophages, was evaluated. Results: The results indicated the highly potent activity of F. tabasensis extracts and F1-F4 fractions, alone or in combination with ShCE, against L. major promastigotes and amastigotes in a dose-dependent manner (P < 0.05). The F1 fraction and methanol extract showed markedly higher toxicity compared to the other extracts and fractions, with inhibitory concentrations (IC50/72h) of 2.4 ± 0.29 and 2.9 ± 0.55 µg/mL against promastigotes and 1.79 ± 0.27 µg/mL and 1.39 ± 0.27 µg/mL against amastigotes (P < 0.001). Moreover, they had a high selectivity index (SI) due to the low toxicity of macrophages (P < 0.0001). The results of flow cytometry indicated that the percentages of apoptotic promastigote cells in contact with IC50 concentrations of F1 and methanol extract alone after 72 h were 43.83 and 43.93%, as well as 78.4%, and 65.45% for their combination with ShCE, respectively. Also, apoptosis of infected macrophages induced by F1 and methanol extracts was estimated at 68.5% and 83.7%, respectively. Conclusions: In this study, the F1 fraction and methanol extract of F. tabasensis showed potent efficacy against L. major, associated with low toxicity and apoptosis induction. Therefore, they can be promising therapeutic candidates in future animal and even human studies.

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Effect of Eucalyptus globulus and Ferula assafoetida essential oils and their nanoformulations on the life table parameters of Tetranychus urticae (Acari: Tetranychidae)

Saber,

Mokhtari

2024

Exp Appl Acarol

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Chemical composition, antileishmanial, and cytotoxic effects Ferula macrecolea essential oil against Leishmania tropica

Cited by 7 publications

References 32 publications

Chemical composition, anti-fungal and cytotoxic effects of Ferula macrecolea essential oil against Candida albicans resistant and sensitive strains

Chemical composition, anti-fungal and cytotoxic effects of Ferula macrecolea essential oil against Candida albicans resistant and sensitive strains

In vitro Evaluation of the Potent Antileishmanial Activity of Ferula tabasensis Alone or in Combination with Shark Cartilage Extract Against the Standard Iranian Strain of Leishmania major (MRHO/IR/75/ER)

Effect of Eucalyptus globulus and Ferula assafoetida essential oils and their nanoformulations on the life table parameters of Tetranychus urticae (Acari: Tetranychidae)

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