Chemical composition, antimicrobial and antibiofilm activity of the essential oil and methanol extract of the Mediterranean cypress (Cupressus sempervirens L.)

Abstract

BackgroundCupressus sempervirens is a medicinal plant traditional, its dried leaves are used in treatment of stomach pain, diabetes, inflammation, toothache, laryngitis and as contraceptive.MethodsThe present study was conducted to evaluate the in vitro antimicrobial, antibiofilm and determination chemical contents of the essential oil (Eo) and methanol extract from Mediterranean C. sempervirens L. The chemical composition of a hydrodistilled Eo of C. sempervirens was analyzed by a GC and GC/MS system.ResultsA total of 20 constituents representing 98.1% of the oil were identified: α-pinene (48.6%), δ-3-carene (22.1%), limonene (4.6%) and α-terpinolene (4.5%) were the main components comprising 79.8% of the oil. The antimicrobial test results showed that the methanol extract of C. sempervirens strongly inhibited the growth of the test bacteria studied, except for yeast species while the Eo had moderate antibacterial, but no anti-candida activity. Klebsiella pneumoniae was proven to be the most susceptible against methanol extract. The exposure time of Eo and methanol extract for complete inhibition of cell viability of K. pneumoniae was found to be 250 μg at 30 min and 500 μg at 120 min, respectively. The antibiofilm potential of the samples was evaluated using methods of PVC microtiter and eradication on biomaterial. Visual results showed visible biofilm eradication from the surface of intravenous infusion tube at 500 μg of Eo and methanol extract.ConclusionsThe results presented here may suggest that the Eo and extracts of C. sempervirens possess antimicrobial and antibiofilm properties, and therefore, can be used as natural preservative ingredients in food and/or pharmaceuticals.

Identification of olfactory receptor neurons in Uraba lugens (Lepidoptera: Nolidae) and its implications for host range

“…Amount produced by each group of plants a (Ogunwande et al, 2005;Viturro et al, 2003;Tsiri et al, 2003;Betts, 2000;Elaissi et al, 2010;Pino et al, 2002;Li et al, 1994;Batish et al, 2006;Li et al, , 1996Bignell et al, 1997), Corymbia (Pino et al, 2002;Bignell et al, 1997;Brophy et al, 1998), Lophostemon (Brophy et al, 2000), Pinus (Yassaa et al, 2000;Petrakis et al, 2001;Ucar and Balaban, 2004;Idzojtic et al, 2005;Yu et al, 2004;Lee et al, 2005;Roussis et al, 2001), Pseudotsuga (Rudloff, 1971;Rudloff and Rehfeldt, 1979;Snow et al, 2003;Adams et al, 2012), Cupressus (Selim et al, 2014;Cheraif et al, 2007;Pierre-Leandri et al, 2003), Fagus (Tollsten and Müller, 1996), Betula Vuorinen et al, 2005;Ibrahim et al, 2010), Populus (Ibrahim et al, 2010;Danner et al, 2011). b The number of values averaged, and the number of publications referred (in parenthesis).…”

Antibiofilm activity of natural substances derived from plants

“…The methanolic extract and the essential oil showed a significant decrease in the fixation of K. pneumoniae in polyvinyl chloride (PVC), material used in the confection of medical catheters. The antimicrobial activity of these compounds can be mostly attributed to their phenolic constituents, α-pinene and cedrol (Selim et al, 2014). Extracts of coconut shell fibers (Cocos nucifera) increased the hydrophobicity of Pseudomonas sp.…”

Evaluation of antibacterial effect of Cyperus species on typical food-borne pathogens

“…They were maintained on agar slant at 4 °C and subcultured on a fresh appropriate agar plate for 24 h prior to any antimicrobial test. MHA was used for the activation of bacteria and the MHB was used for the minimum inhibitory concentration (MIC) determinations [10]. Finally, suspensions were adjusted to 0.…”