Chemical composition, antimicrobial, and lipase enzyme activity of essential oil and solvent extracts from Serapiasorientalis subsp. orientalis

Abstract

The volatile component of essential oil (EO), SPME, and SPME of solvent extracts (n-hexane, methanol and water) obtained from fresh Serapias orientalis subsp. orientalis (Soo) were analyzed by GC-FID/MS. EO of Soo gave eleven compounds in the ratio of 99.97% and capronaldehyde (37.01%), 2-(E)-hexenal (23.19%), and n-nonanal 2 (19.05%) were found to be major constituents. SPME GC-FID/MS analyses of fresh plant and solvent extracts of Soo revealed 7, 12, 7, and 4 compounds within the range of 99.7% to 99.9% ratio, respectively. Limonene (76.5%, 41.7% and 61.3%) was the major compound in SPME and SPME of the n-hexane and methanol extracts, respectively. α-Methoxy-p-cresol (52.9%) was the main component in water extract of it. The antimicrobial activity of EO and the solvent extracts of Soo were screened for against nine microorganisms. EO showed the best activity against the Mycobacterium smegmatis with 79.5 µg/mL MIC value. The nhexane, methanol, and water extracts were the most active against the Staphylococcus aureus within the range of 81.25-125.0 µg/mL (MIC). IC 50 values for the lipase enzyme inhibitory activity of EO and solvent extracts (n-hexane, methanol, and water) was determined to be 59.87 µg/mL 64.03 µg/mL, 101.91 µg/mL, and 121.24 µg/mL, respectively.

Antimicrobial and lipase inhibition of essential oil and solvent extracts of Cota tinctoria var. tinctoria and characterization of the essential oil

“…Blended material (5 g, each) was extracted (25 mL × 3; 12 h each) using maceration method at room temperature with analytical grade n- hexane, acetonitrile, methanol, and water solvents in flasks (50 mL) separately. After the suction filtration, the same extracts were combined and solvents were evaporated or lyophilized to yield crude n- hexane (0.0722 g), acetonitrile (0.0439), methanol (0.5234 g), and water extract (0.0435 g) [ 39 , 40 ].…”

“…RI values of the volatile components in EO of C. tinctoria var. tinctoria were determined by Kovats method ( Table 1 ) [ 39 – 44 ]. Volatile compounds of the EO were identified by comparisons of RI values with those reported in the literature RI [ 45 – 75 ] and MS data matching mass spectral libraries (NIST, Wiley7NL, FFNSC1.2, and W9N11).…”

“…Ampicillin (10 mg/mL), streptomycin (10 mg/mL), and fluconazole (5 mg/mL) were used as standard, respectively. n- Hexane and dimethyl sulphoxide were used as solvent control with dilution of 1:10 [ 39 , 76 – 77 ].…”

“…Lipase inhibitory assay for the EO and solvent extracts ( n- hexane, acetonitrile, methanol, and water) of C. tinctoria var . tinctoria were studied with the modified method using p- nitrophenyl butyrate ( p- NPB) as substrate [ 39 ]. All extracts (25, 100, 200, and 400 μg/mL concentrations) were dissolved with buffer solution (0.1 M Tris-HCl, pH = 8.0) and 0.1% DMSO.…”

The efficiency of MALDI-TOF MS method in detectingStaphylococcus aureusisolated from raw milk and artisanal dairy foods

“…The reason for S. aureus-negative Maras ice cream samples can probably be because of the proven antimicrobial effect of bioactive compounds (capronaldehyde, 2(E)-hexenal, limonene, heptanal, pelargonaldehyde, decane, and etc.) of salep orchids, of its storage at such low temperatures -unlike other dairy products, and of greater care that is taken in handling them (Erik et al, 2020). However, this must not lead to lowering of hygiene standards during the production, packaging, storage, and distribution of these dairy foods.…”