Chemical composition, antitumor and antioxidant effects of four lebanese plants extracts on human pulmonary adenocarcinoma

Abstract

All samples were collected in full flowering stage. The plant identification was based on 'Nouvelle Flore du Liban et de la Syrie' by Paul Mouterde and voucher specimen were deposited at the Herbarium of the Faculty of Agricultural Sciences -USEK with their numbers. Prangos asperula (MNII 265a) and Hirtellina lobelii (MNIII 145b) were collected from "Tannourine" region in the North of Lebanon, Salvia multicaulis ((MNIII 475a) was collected from "Mdayrej" (Zahleh region) and Stachys ehrenbergii (MNIII 235c) from "Jabal El Makmel" (Arez, North of Lebanon). Methanolic extractionA 99.9% methanol HPLC Grade was used in order to dissolve the plants and to extract the active compounds. 50 g of dried aerial parts of the plants were macerated in 500 ml of methanol for 72 hours. All the extracts were macerated at room temperature, and then stored at C. Extracts analysisIn order to evaluate the chemical composition of each extract, GC-MS was used. Briefly, concentrated and diluted samples with methanol were tested in the machine. GC/MS was performed using an Agilent gas chromatograph 6890 coupled with Mass Detector 5975. The 7683 B auto sampler was injecting each time 1 µL of extract sample. GC/MS analysis was carried out using a fused silica capillary column Factor DB-5 MS, measuring 30 m x 0.25 mm internal diameter, film thickener of 0.1 µm; the oven temperature program adopted was 35 ˚C with an increase of 5˚C /min until 85˚C (20 min) and then to

Genus Stachys: A Review of Traditional Uses, Phytochemistry and Bioactivity

“…japonica exhibited antioxidant effects on ultraviolet A (UVA)-irradiated human dermal fibroblasts (HDFs), through suppression of ROS generation [ 160 ]. The antioxidant activity of the methanol extract of the Lebanese species S. ehrenbergii was measured by ABTS radical cation decolorization assay and the methanol extract showed an IC 50 value of 52 ± 7.5 mg/mL [ 154 ]. Furthermore, the chemical profile and some biological activities of three herbal teas in Anatolia were examined [ 84 ].…”

“…They reported that all the plant extracts induced DNA damage, using the comet assay, whereas the extract of S. officinalis induced the increase of sister chromatid exchange value. The methanol extract of the Lebanese species S. ehrenbergii was investigated for its antioxidant and cytotoxic activity [ 154 ]. The cytotoxicity was examined by MTT assay where the methanol extract showed the highest cytotoxicity (IC 50 = 420 ± 104 μg/mL) at a concentration of 3000 mg/mL.…”

Stachys schtschegleevii tea, matrix metalloproteinase, and disease severity in female rheumatoid arthritis patients: a randomized controlled clinical trial

“…Indeed, FLS is a significant source incorporated in the production of different MMPs [3,7]. Therefore, according to experimental in vitro studies, one can assume that SSC and other Stachys, when they have exhibited antiproliferative and pro-apoptotic effects [22,[31][32][33], might reduce the number of FLSs and likely help to modulate the levels of MMP production in FLSs [5,8]. Findings of the current randomized clinical trial are only representative (generalizability of data) to a group of RA patients who have 3.2≤DAS28≤5.1 in terms of inferring the moderate activity of RA disease.…”

“…The anti-inflammatory potential of SSC could be partly explained by inhibiting some enzymes (e.g., cyclooxygenase and lipoxygenase) [16,30]. Critical to this, the anti-proliferative activities of Stachys is another implication that has been emerged by in vitro studies [31][32][33],…”

The Protective Effects of Daucus carota L. ssp. carota and Cannabis sativa L. Extracts against Cisplatin Induced Nephrotoxicity in Animal Models. (c2021)

“…multicaulis and H. lobelia, have antitumor and antioxidant effects on human pulmonary adenocarcinoma cells (Abi-Rizk et al, 2020). Still, more studies need to be conducted to prove the medicinal benefits of Lebanese natural products.…”