Chemical Composition of Argentinean Propolis Collected in Extreme Regions and its Relation with Antimicrobial and Antioxidant Activities

Abstract

This paper reveals, for the first time, the functional properties of propolis from an extreme region of Argentine (El Rincón, Province of Catamarca, Argentina), as well as the isolation and identification of bioactive compounds. The antioxidant activity was determined by the ABTS method and β-carotene bleaching. The antibacterial activity was determined on methicillin resistant Staphylococcus aureus (MRSA) by the microdilution method and bioautographic assays. Twelve compounds were isolated and identified by NMR spectroscopy. The main bioactive compounds were 2',4'-dihydroxy-3'-methoxychalcone (3), 2',4'-dihydroxychalcone (9), 2',4',4-trihydroxy-6'-methoxychalcone (8), 5-hydroxy-4',7-dimethoxyflavone (4) , 4',5-dihydroxy-3,7,8-trimethoxyflavone (10) and 7-hydroxy-5,8-dimethoxyflavone (11). All compounds were active against clinical isolates (MIC 50 10 μg/ mL) and displayed antioxidant activity (SC 50 values of 20 μg/mL). The MIC and SC 50 values of the isolated compounds were lower than those obtained with crude propolis extracts, chloroform sub-extracts and isolated fractions.

Chemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials

“…EPA and EPB inhibited significantly (100%) the clinical strains of MRSA and of S. aureus ATCC 6538 and ATCC 25923. Our results were similar to others reports in relation to the MRSA (Vera et al 2011, Astani et al 2013, Aguiar et al 2014) and S. aureus ATCC 6538 (Hazem et al 2017). In opposition, AL-Ani et al (2018) observed a moderate anti-MRSA efficacy and Katircioglu & Mercan (2006) a low efficacy against S. aureus ATCC 25923, reinforcing the importance of the evaluation of multiresistant strains in the tests of antimicrobial activity of natural products (Cos et al 2006).…”

Potentiality of standardized extract and isolated flavonoids from Zuccagnia punctata for the treatment of respiratory infections by Streptococcus pneumoniae: In vitro and in vivo studies

“…The results indicate that these structures are the most favourable for antibacterial activity within the flavonoid family (Zampini et al, 2005;Agüero et al, 2010;Vera et al, 2011). The same effect was obtained with other chalcones and flavanones previously investigated (Olivella et al, 2001;Nowakowska, 2007;Agüero et al, 2010;Vera et al, 2011). In this work, the anti-streptococcal in vitro activities of Zuccagnia punctata crude extract and HF were also observed in vivo when their activities were evaluated in a Streptococcus pneumoniae infection model in mice.…”

“…The highest in vitro activity of isolated compounds was observed for assayed chalcone and flavanone (DHC and HF, respectively). The results indicate that these structures are the most favourable for antibacterial activity within the flavonoid family (Zampini et al, 2005;Agüero et al, 2010;Vera et al, 2011). The same effect was obtained with other chalcones and flavanones previously investigated (Olivella et al, 2001;Nowakowska, 2007;Agüero et al, 2010;Vera et al, 2011).…”

Korean Propolis Suppresses Angiogenesis through Inhibition of Tube Formation and Endothelial Cell Proliferation

“…It has been used in folk medicine from ancient times for its pharmaceutical properties and has been extensively studied in many countries [4,5]. It is reported to possess various biological activities, such as antibacterial [6,7], antiviral [6,8], antifungal [6,9], antiinflammatory [10], antihepatotoxic [11], anticancer [12,13], antitumor [14,15], and antimutagenic [16] properties. For these reasons, it is extensively used in foods, beverages, and dietary supplements to improve health and to prevent diseases [5,17].…”