Hibiscus cannabinus is used in traditional medicine for treatment of cancer, bacterial infections, diabetics and other diseases. The study was carried out to investigate the phytochemical and antioxidant properties of the oil extracted from Hibiscus cannabinus. The physicochemical parameters of the seed powder were determined using standard procedures. The oil was extracted using Soxhlet extractor and the phytochemical composition was analysed using qualitative tests and GC-MS. The organoleptic characters of the oil were evaluated using standard protocols. Oral acute toxicity in Wistar rats was assayed using the method outlined by the Organization for Economic Co-operation and Development (OECD 425). Qualitative and quantitative antioxidant activity were determined using DPPH free radical assay. The result of the physicochemical evaluation revealed moisture content (8.0%), total ash (4.0%), acid insoluble ash (5.0%), alcohol extractive value (5.5%) and water extractive value (4.5%). The percentage yield of the oil was 13.2%. The organoleptic evaluation of the oil revealed characteristic taste, yellowish colour, characteristic smell and smooth texture. The oil had specific gravity of 0.94 and was found to be soluble/miscible in some organic solvents. The preliminary phytochemical screening revealed the presence of steroids/triterpenoids. The GC-MS revealed the presence of some fatty acids and their esters. The acid value (65.64 mgKOH/g of oil), saponification value (159.32 mgKOH/g of oil), ester value (93.68 mgKOH/g of oil) and iodine value (11.40 gI2/100 g of oil) were obtained. The oral acute toxicity showed that the oil was not toxic up to a dose of 5000 mg/kg per body weight. The oil demonstrated free radical scavenging activity with highest percentage inhibition of 85.30% at 0.441 μg/mL. The results of the research showed that Hibiscus cannabinus oil contain antioxidant compounds and the oil is not toxic when tested in rats. Therefore, the oil can be used in food and pharmaceutical industries.