At present, there is no reliable chemical standardization of mumijo (moomiyo), which is a complex organomineral natural product. Previously we have attempted to develop a system of biological standardization of the dry mumijo extract (DME) [1,2]. Now we are continuing investigations of the physicochemical properties of both raw mumijo materials and the preparations based on the mumijo. The results of these studies allowed us to suggest that the content of lipid fatty acids may serve as one of the criteria for assessment of the true natural origin and good quality of DME samples [3].An analysis of data available in the literature [4] showed that all the previously studied mumijo samples, representing various deposits, were reported to contain amino acids. However, the analyses were performed on samples differing by the degree of purification, using different analytical procedures and reagents. This circumstance hinders using these data for the further development of criteria of the mumijo quality.The purpose of this work was to study composition of the amino acid component of mumijo.Experiments were performed on a series of more than 50 DME samples extracted using the same procedure from raw mumijo materials obtained from various regions both in Russia and abroad, including Altai-Sayan, Pamir-Altai, MiddleAsia, Kazakh, and Mongol; also studied was the DME-based Shiladjit drug purchased from Dabur company (India).The DME samples were prepared under commercial conditions by the research and production company Sibdalmumijo (Novokuznetsk). The procedure involved multiply repeated extraction of the comminuted raw materials with. water, followed by drying to the state of dry extract.
EXPERIMENTAL PARTThe experiments were performed in the Belozerskii Research Institute of Physicochemical Biology at the Moscow State University.
I Research Institute of Traditional Curing Methods, Ministry of Health andMedicinal Industries of the Russian Federation, Moscow, Russia.
103The qualitative and quantitative analyses of the amino acid component of DME were performed by the method of ion-exchange liquid chromatography. The measurements were conducted on a High-Speed Amino Acid Analyzer Model 835 (Japan) equipped with Hitachi Custon Ion-Exchange columns having a length of 1.5 m and an internal diameter of 2.6 m, filled with a Grade 2619 cation exchanger resin. The samples were eluted with a sodium citrate buffer (pH 5.5) in a stepwise mode and detected with a ninhydrin solution (pH 5.5) in the presence of a reducing agent (tin or titanium chloride).Amino acids were analyzed under standard conditions used for the separation of protein hydrolyzates [5] and identified by the retention times compared with those for an internal standard comprising a mixture of 18 amino acids.The parameters of chromatograms were processed and interpreted using a computer-based system automatically determining areas under the peaks of identified amino acids and the retention times, peak areas, and absorption characteristics of the unknown compounds. The con...