Chemical composition of Ocimum sanctum by LC-ESI–MS/MS analysis and its protective effects against smoke induced lung and neuronal tissue damage in rats

Venuprasad, M. P.; Kandikattu, Hemanth Kumar; Razack, Sakina; Amruta, Narayanappa; Khanum, Farhath

doi:10.1016/j.biopha.2017.04.011

Cited by 30 publications

(16 citation statements)

References 39 publications

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“…The chemical composition of EEOS has been reported (Venuprasad et al, 2017). They found that EEOS was composed of several phenolic compounds and flavonoids, including rosmarinic acid and apigenin.…”

Section: Discussionmentioning

confidence: 99%

Ethanolic Extract of Ocimum sanctum Leaves Reduced Invasion and Matrix Metalloproteinase Activity of Head and Neck Cancer Cell Lines

Utispan

Niyomtham

Yingyongnarongkul

et al. 2020

Asian Pac J Cancer Prev

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Background: Head and neck squamous cell carcinoma (HNSCC) has a yearly incidence of 600,000 cases worldwide with a low survival rate. Ocimum sanctum L. or Ocimum tenuiflorum L. (Holy basil; Tulsi in Hindi), is a traditional medicine herb that demonstrates numerous effects including anti-oxidant, anti-microbial, and anti-tumor effects. The aim of this study was to evaluate the anti-invasive effect of O. sanctum leaf extract on HNSCC cell lines. Methods: Ethanolic extract of O. sanctum leaf (EEOS) was prepared and the phenolic compounds were identified using high-performance liquid chromatography-electrospray ionization-time of flight-mass spectrometry. Genetically matched HNSCC cell lines derived from primary (HN30 and HN4) and metastatic sites (HN31 and HN12) from the same patient were used in this study. The EEOS cytotoxicity to the cell lines was determined using an MTT assay. The invasion and matrix metalloproteinase (MMP)-2 and-9 activity of EEOS-treated cells were tested using a modified Boyden chamber assay and zymography, respectively. Results: We found that EEOS significantly inhibited the invasion and MMP-2 and MMP-9 activity of HN4 and HN12 cells, but not HN30 and HN31 cells. Rosmarinic acid, caffeic acid, and apigenin were detected in EEOS. Moreover, rosmarinic acid was found as the major phenolic compound. Conclusion: EEOS exerted its anti-invasive effect on HNSCC cells by attenuating MMP activity. The active compounds identified in EEOS might be promising as an alternative therapeutic agent for HNSCC.

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Section: Discussionmentioning

confidence: 99%

Ethanolic Extract of Ocimum sanctum Leaves Reduced Invasion and Matrix Metalloproteinase Activity of Head and Neck Cancer Cell Lines

Utispan

Niyomtham

Yingyongnarongkul

et al. 2020

Asian Pac J Cancer Prev

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“…The chemical composition of EEOS has been reported [29]. They found that EEOS was composed of several phenolic compounds and flavonoids, including rosmarinic acid and apigenin.…”

Section: Discussionmentioning

confidence: 99%

Ethanolic extract of Ocimum sanctum leaves reduced invasion and matrix metalloproteinase activity of head and neck cancer cell lines

Utispan

Niyomtham

Yingyongnarongkul

et al. 2019

Preprint

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2Head and neck squamous cell carcinoma (HNSCC) has a yearly incidence of 600,000 3 cases worldwide with a low survival rate. Ocimum sanctum L. or Ocimum tenuiflorum L. (Holy 4 basil; Tulsi in Hindi), is a traditional medicine herb that demonstrates numerous effects, 5 including anti-oxidant, anti-microbial, and anti-tumor effects. The aim of this study was to 6 evaluate the anti-invasive effect of O. sanctum leaf extract on HNSCC cell lines. Ethanolic 7 extract of O. sanctum leaf (EEOS) was prepared and the phenolic compounds were identified 8 using high-performance liquid chromatography-electrospray ionization-time of flight-mass 9 spectrometry. Genetically-matched HNSCC cell lines derived from primary (HN30 and HN4) 10 and metastatic sites (HN31 and HN12) from the same patient were used in this study. The EEOS 11 cytotoxicity to the cell lines was determined using an MTT assay. The invasion and matrix 12 metalloproteinase (MMP)-2 and -9 activity of EEOS-treated cells were tested using a modified 13 Boyden chamber assay and zymography, respectively. We found that EEOS significantly 14 inhibited the invasion and MMP-2 and MMP-9 activity of HN4 and HN12 cells, but not HN30 15 and HN31 cells. Rosmarinic acid, caffeic acid, and apigenin were detected in EEOS. Moreover, 16 rosmarinic acid was found as the major phenolic compound. Therefore, EEOS exerted its anti-17 invasive effect on HNSCC cells by attenuating MMP activity. 18 2 23 aggressiveness [3]. Matrix metalloproteinase (MMPs) are the key enzymes involved in tumor 24 invasion and metastasis. MMP-2 and MMP-9 destroy the basement membrane and degrade the 25 extracellular matrix, promoting tumor invasion [4]. Although modern medicine has contributed 26 to treating cancers by surgery, chemotherapy, and radiotherapy, these modalities have not 27 significantly changed the survival rate over the past three decades [5]. Thus, more effective 28 treatments for local and metastatic HNSCC are needed. 29 Ocimum sanctum Linn. or Ocimum tenuiflorum Linn., commonly known as Holy Basil in 30 English or Tulsi in Indian language [6], is a highly potent medicinal herb that is native 31 throughout the eastern tropical countries including Thailand [7, 8]. O. sanctum is primarily 32 composed of phytochemicals [9]. The fresh leaves and stem contain several flavonoids and 33 phenolic compounds. Phenolic compounds such as apigenin, rosmarinic acid, cirsilineol, 34 cirsimaritin, isothymusin, and isothymonin have been detected in O. sanctum leaf extracts [10]. 35 The phytochemicals in this plant varies depending on different growing, harvesting, extraction, 36 and storage conditions [11]. The leaf extracts of O. sanctum have numerous medicinal effects 37 such as anti-oxidant [12, 13], wound healing [14], anti-microbial [15] and anti-tumorigenic 38 effects [11]. The ethanol extract of O. sanctum leaf (EEOS) has demonstrated anti-tumorigenic 39 effects on several cancer types including gastric cancer [16], pancreatic cancer [17], non-small 40 cell lung cancer [18], and lung cancers [19, 20]...

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“…HEK-293 is a cell line which derived in 1973 from primary human embryonic kidney cells transformed by sheared adenovirus 5 DNA [ 18 ]. It has a general molecular pattern of intermediate filament protein expression similar to that seen in PC12 cells and Ntera-2 cells, two widely used neuronal lineage cell lines [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…Liquid chromatography - electrospray ionization - mass spectrometry shows that fraction of O. sanctum Linn. composed of eugenol, luteolin, apigenin, and ursolic acid [ 18 ]. Those metabolites are potential to be explored for the drugs development.…”

Section: Introductionmentioning

confidence: 99%

“…has been reported for its protective effect against some induced oxidative stress that causes various cancers and associated health problems. The administration of O. sanctum helps to regulated neurotransmitter levels which play an essential role in neuronal function [ 18 ]. On the in vitro studies, OSE was able to maintain the stability of ChAT expression at human cerebral microvascular endothelial cells (HCMECs) mimic young age and restore ChAT expression at mimic old age on in vitro model [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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The neuroprotective effect of Ocimum sanctum Linn. ethanolic extract on human embryonic kidney-293 cells as in vitro model of neurodegenerative disease

Hening

Wijayanti

et al. 2018

Vet World

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Aim:This study aimed to analyze the neuroprotective effect of Ocimum sanctum Linn. ethanolic extract (OSE) on human embryonic kidney-293 (HEK-293) cells as the in vitro model of neurodegenerative diseases.Materials and Methods:In this research, HEK-293 cells divided into five groups consisting of normal and healthy cells (NT), cells treated with Camptothecin 500 µM as the negative control, cells treated with trimethyltin 10 µM (TMT), cells treated with OSE 75 µg/ml, and cells pre-treated with OSE 75 µg/ml then induced by TMT 10 µM (OSE+TMT). MTT assay and phase contrast microscopy were applied to observe the cell viability quantitatively and morphological after Ocimum sanctum Linn extract treatment. Finally, the reverse transcription polymerase chain reaction was employed to study the expression of choline acetyltransferase (ChAT).Results:The MTT assay and phase contrast microscopy showed that OSE pre-treatment significantly increased the viability of TMT-induced apoptotic cells and maintained cell viability of the normal HEK-293 cells. Expression of ChAT markedly reduced on TMT treatment group, but OSE administration stabilized ChAT expression in TMT-induced HEK-293 cells.Conclusion:This present study proved that OSE administration has neuroprotective effect by increased HEK-293 cells viability and maintain ChAT expression.

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Chemical composition of Ocimum sanctum by LC-ESI–MS/MS analysis and its protective effects against smoke induced lung and neuronal tissue damage in rats

Cited by 30 publications

References 39 publications

Ethanolic Extract of Ocimum sanctum Leaves Reduced Invasion and Matrix Metalloproteinase Activity of Head and Neck Cancer Cell Lines

Ethanolic Extract of Ocimum sanctum Leaves Reduced Invasion and Matrix Metalloproteinase Activity of Head and Neck Cancer Cell Lines

Ethanolic extract of Ocimum sanctum leaves reduced invasion and matrix metalloproteinase activity of head and neck cancer cell lines

The neuroprotective effect of Ocimum sanctum Linn. ethanolic extract on human embryonic kidney-293 cells as in vitro model of neurodegenerative disease

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