Chemical Composition of the Essential Oil from Annona crassiflora

Abstract

Annona crassiflora is a species native to the cerrado region but also found in other regions of Brazil. The species belongs to the family of Annonaceae and their fruit is popularly known as araticum, ariticum, and marolo [1]. The species is used in folk medicine in the treatment of wounds and snakebites, and as an antimicrobial and antidiarrheal [2]. Some studies have shown that this species has antioxidant and cytotoxic activity [3,4].The objective of this study was to determine the chemical composition of the essential oil from different parts at different times of A. crassiflora collected in southern Brazil.The leaves, fruits, and flowers of A. crassiflora were collected in the town of Tres Rios, Brazil in 2010. A voucher specimen (SMDB 12.938) has been deposited in the Herbarium of the UFSM. The fresh leaves were submitted to hydrodistillation (4 h) to yield ca. 0.3% of oil. The oil was submitted to GC analysis in a Shimadzu (GCMS-QP2010 Plus) instrument equipped with a capillary fused silica column (30 m u 0.25 Pm) coated with RTx-5MS. The injector and detector temperatures were 240 and 260qC, respectively. He was used as carrier gas at a flow rate of 1 mL min -1 , injection was in the split mode (1:10), and the injection volume was 1 mg mL -1 of a solution containing withdraw oil in hexane. MS spectra were obtained using electron impact at 70 eV with a scan interval of 2.94 scan s -1 . Compound identification was based on the comparison of retention indexes with the same columns and mass spectra described [5,6]. Retention indices (RI) were obtained according to the method of Van den Dool relative to C7-C30 saturated n-alkanes standard. Quantitative analyses of the chemical constituents was performed using a flame ionization detector (FID) using a Shimadzu (GC-2010) instrument under the same conditions and with the same column as reported for the GC-MS. Table 1 lists the essential oils of the compounds obtained from A. crassiflora. Twenty-five compounds were identified, representing 94.0% of the oils.The essential oil of A. crassiflora consists mainly of oxygenated sesquiterpenes. Nerolidol was found in the highest concentration in all the oils analyzed. The oil from the leaves collected in summer showed a higher concentration of nerolidol (57.1%), and the oil of the fruit collected in summer showed the lowest concentration (18.7%).The essential oil obtained from the fruit showed no sesquiterpene hydrocarbons. Octanol acetate, epi-longipinalol, spathulenol, and dihydromyrcene comprises the majority of the fruit essential oil.The leaves collected in the spring contained spathulenol and caryophyllene oxide as major compounds. The oils collected from leaves (summer and autumn) show '-elemene and E-caryophyllene in higher concentrations than other oils, and E-caryophyllene was found in the leaves in these two seasons.

Óleo Essencial De Copaíba (Copaifera Langsdorffii Desf.) No Tratamento De Mastite Bovina

“…ANOVA indicated interaction between the two factors only for a-pinene and methyl hexanoate, with the highest contents at around six months of storage for mixed (16.1%) and marolo ( were predominant in SDE, and ethyl butyrate (7.4%) and ethyl hexanoate (2.5%) in SPME. Marolo has previously shown mainly oxygenated sesquiterpenes (SIRENA et al, 2014). However, in this study, ripe fruit pulp extracted by SPME showed only esters, mainly methyl and ethyl derivatives of hexanoate, octanoate and decanoate (SILVA et al, 2013).…”

AVALIAÇÃO IN VITRO DE ÓLEOS ESSENCIAIS DE COPAÍBA, BURITI E TUCUMÃ PARA CONTROLAR Sthapylococcus aureus

“…were predominant in SDE, and ethyl butyrate (7.4%) and ethyl hexanoate (2.5%) in SPME. Marolo has previously shown mainly oxygenated sesquiterpenes (SIRENA et al, 2014). However, in this study, ripe fruit pulp extracted by SPME showed only esters, mainly methyl and ethyl derivatives of hexanoate, octanoate and decanoate (SILVA et al, 2013).…”

“…GONZÁLEZ et al, 2005) and terpenes or esters as major volatiles (PERALTA-BOHÓRQUEZO et al, 2010). EOs from marolo have mainly terpenes (SIRENA et al, 2014). In turn, fresh pulp show changes in terpene and ester compositions, with only esters at the end of fruit ripening (SILVA et al, 2013), whereas lyophilized pulp provides esters and alcohols as its main constituents (BEZERRA et al, 2018).…”

Fenologia reprodutiva do araticum e suas implicações no potencial produtivo

“…Os frutos in natura são apreciados pelas comunidades locais e aproveitados por meio da produção de sucos, sorvetes e geléias (Luzia & Jorge, 2012). Além da riqueza nutricional presente na polpa do fruto (Cardoso et al, 2013;Siqueira et al, 2013), a fração lipidica nas sementes pode ser usada na indústria farmacêutica e de alimentos e para fabricação de biodiesel (Egydio & Dos Santos, 2011;Luzia & Jorge, 2012;Sirena et al, 2014).…”