KEY WORDS: Micromeria persica; Labiatae; essential oil composition; thymol; γ-terpinene; limonene; 1,8-cineole; p-cymene; Iran hydrodistillation for 2.5 h using a Clevenger-type apparatus. The essential oils were obtained in 3.00% and 3.20% (w/w) yield, before the flowering and full flowering stages, respectively. The oils were dried over sodium sulphate and stored in sealed vials at low temperature before analysis.
Gas ChromatographyGC analyses were performed using a Shimadzu GC-9A gas chromatograph equipped with a DB-5 fused silica column (30 m × 0.25 mm i.d., film thickness 0.25 µm). Oven temperature was held at 40°C for 5 min and then programmed to 250°C at a rate of 4°C/min. Injector and detector (FID) temperature, 260°C; helium was used as carrier gas at a linear velocity of 32 cm/s.
Gas Chromatography-Mass SpectrometryGC-MS analyses were carried out on a Varian 3400 GC-MS system equipped with a DB-5 fused silica column (30 m × 0.25 mm i.d.); oven temperature, 40°C to 240°C at a rate of 4°C; transfer line temperature, 260°C; injector temperature, 250°C; carrier gas, helium at a linear velocity of 31.5 cm/s; split ratio, 1/60; flow rate, 1.1 ml/min; ionization energy, 70 eV; scan time, 1 s; mass range, 40-350 amu.