“…The calyxes of karkade are rich in bioactive compounds including organic acids, phenolic compounds, vitamin A and vitamin C [2], flavonoid (anthocyanins), gossypetin, quercetin, pectin [3], saponins, glycosides, and alkaloids [4]. Additionally, Nnam and Onyeke [5] mentioned that calyxes contain 6.4% protein, 79.25% carbohydrates, 5.13% fat, 2.7% crude fiber, and 6.52% ash. Fresh or dried calyxes of karkade have been used traditionally as herbal drinks (cold and hot) because karkade has different therapeutic properties such as anticancer, antiviral, antifungal, antibacterial, antioxidant, antidiabetic, antihypertensive, antihyperlipidemia (anticholesterol), antiobesity, antispasmodic, and antitumor effects, decreasing the viscosity of the blood and stimulating intestinal peristalsis, and can be used to treat sore throats, coughs, and genital problems and it has also nephro/hepatoprotective and renal/diuretic effect and so forth [6].…”