Chemical compositions and antimicrobial activities of essential oils extracted from Acanthopanax brachypus

Abstract

The essential oils from the flowers, leaves, barks, roots and fruits of A. brachypus were individually extracted by hydrodistillation, and their chemical constituents were isolated and characterized by means of gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). A total of 160 different constituents accounting for 86.45-96.54% of the total essential oils were identified, and 3 constituents were unidentified (< 0.8%), and significant qualitative and quantitative differences were observed among the oils, in which the yields varied between 0.61-1.83 (mL/100 g dried materials) under the same operational conditions. The oils from flowers, leaves, barks and roots consisted mainly of monoterpenes and sesquiterpene hydrocarbons as well as oxygenated monoterpenes and sesquiterpenes, while the fruit oil consisted mainly of fatty acids and esters. The antimicrobial activities of the essential oils was evaluated against 11 microorganisms (9 for bacteria and 2 for yeast) using agar disc diffusion and broth microdilution methods. The bacteria, including gram-positive bacteria and gram-negative bacteria, were more sensitive to the oils than yeasts.

Antibacterial activity of &#945;-terpineol may induce morphostructural alterations in Escherichia coli

“…Measured the values of OD 600 of α-terpineol by UV spectrophotometer and measured once again after 24 h of incubation at 37 °C. The test tube with the same value of OD 600 after 24 h, showing that there were no Escherichia coli colonies growing that is the value of MIC ( Hu. et al , 2009 ).…”

Chemical composition and antimicrobial activities of volatile oil extracted from Chrysanthemum morifolium Ramat.

“…Individual or combinations of plant essential oils may provide an efficacious mixture for the inactivation of pathogenic and spoilage microorganisms, and to achieve adequate shelf-life of foods. Hu et al (2009) analyzed the volatile oils from the flowers, leaves, barks, roots and fruits of A. brachypus were extracted individually by hydro distillation, and their chemical constituents were isolated and characterized by means of GC-MS. The antimicrobial activities of the volatile oils was evaluated against 11 microorganisms (9 strains bacteria and 2 strains yeast) using agar disc diffusion and broth micro-dilution methods.…”

Cloning and functional characterization of a monoterpene synthase gene from Eleutherococcus trifoliatus

“…EtLIM was active with GPP but not with FPP. The major product of EtLIM was identified as limonene (Figure 3), based on comparison of the retention time, KI value, and mass spectra from a study that investigated the composition of the essential oil extract from Acanthopanax brachypus (Hu et al 2009). BcLIS is a linalool synthase from B. citriodora (BAG82825), SaBIS is a β-bisabolene synthase from S. austrocaledonicum (ADO87003), VvOMS is a (E)-β-ocimene/myrcene synthase from V. vinifera (ADR74206), and LcTHS is an α-thujene/sabinene synthase from L. cubeba (AEJ91556).…”