Chemical compositions and biological activities of marine invertebrates from the East Sea of South Korea

Abstract

Background: Marine invertebrates are well known as pivotal bioresources with bioactive substances such as antiinflammatory sterols, antitumor terpenes, and antimicrobial peptides. However, there are few scientific reports on chemical compositions and bioactivities of marine invertebrates from the East Sea of South Korea. Methods: In this study, chemical compositions and biological activities were evaluated on both 70% EtOH and hot water extracts of 5 species of marine invertebrates (Crossaster papposus japonicus, Actinostola carlgreni, Stomphia coccinea, Actinostola sp., and Heliometra glacialis) collected from the East Sea of South Korea. The antioxidant activities were measured by ABTS radical scavenging assay. The cytotoxicity and anti-inflammatory activity were evaluated using MTT and Griess reagents. Moreover, the antibacterial effect was evaluated using paper disc assay and minimum inhibitory concentration (MIC) assay. Results: In the results of antioxidant activities, 70% EtOH extract of A. carlgreni showed the highest activity (IC 50 0.19 ± 0.03 mg/ml) compared to other extracts. Moreover, 70% EtOH extract of A. carlgreni could significantly suppress the nitric oxide (NO) production in lipopolysaccharide-induced RAW 264.7. All extracts treated under 400 μg/ml have no cytotoxic effects on RAW 264.7 macrophages. In the antibacterial test, both 70% EtOH extracts of C. papposus japonicus and H. glacialis showed a significant antibacterial effect on Staphylococcus aureus. The MIC values were evaluated at 256 and 512 μg/ml, respectively. Conclusions: These results suggested the bioactive potentials of marine invertebrates from the East Sea of South Korea in pharmaceutical and nutraceutical applications.

Therapeutic effect of marine bioactive substances against periodontitis based on in vitro, in vivo, and clinical studies

“…Although MBS has high therapeutic potential on periodontitis, several limitations and weaknesses remain in terms of extraction source, underlying mechanism from in vitro and in vivo, and few results in clinical safety and efficacy. Firstly, although the ocean covers more than 70% of the earth embedding numerous marine organisms with biological agents, only a few marine organisms were used as extraction sources of bioactive substances for periodontitis treatment (Kim et al, 2019a). Next, most studies used commercial products of MBS, not extracted MBS.…”