Distichochlamys benenica is a newly discovered plant belonging to the Distichochlamys family, which exerts many important biological activities. However, the anti-cancer, anti-inflammatory, and anti-oxidant effects of the crude (ME) and fractioned extracts of D. benenica, such as hexane (HE), chloroform (CE), and ethyl acetate extracts (EAE), have not been elucidated yet. The antioxidant activity of the extracts was evaluated via DPPH free radical scavenging and phosphomolybdate assays. The anti-inflammatory potential of the extracts was assessed via their inhibitory effects on protein denaturation and nitric oxide production. The anti-cancer effect of the extracts against A549 and MDA-MB-231 cell lines was determined via MTT assay. The results revealed that among four extracts, the polyphenol concentration was highest in EAE (350.59 ± 13.26 mg GAE/g) while the flavonoid content was highest in HE and ME. Furthermore, the greatest DPPH radical capture efficiency was demonstrated by EAE (89.76 ± 0.68%), and total antioxidant activity was greatest in HE (204.18 ± 3.51 mg AAE/g). The greatest suppression of NO generation was similarly shown by EAE and HE, with IC50 values ranging from 3.72-4.06 µg/mL, respectively. Additionally, HE and EAE exhibited an inhibitory effect on protein denaturation (ranging from 12.76%-28.81%). HE and EAE had the greatest effectiveness against MDA-MB-231 cell lines in the antitumor test. These findings validate the potent antioxidant, anti-inflammatory, and anti-cancer properties of D. benenica extracts, especially EAE and HE, and pave the way for the application of the extracts in the pharmaceutical industry as promising anti-inflammatory and anti-cancer medicines.