Chemical constituents and biological activities of the seed essential oil from Michelia tonkinensis grown in Thanh Hoa province, Viet Nam

Abstract

Essential oils (EOs) from the seed of Michelia tonkinensis were extracted in four ways hydrodistillation (HD), microwave‐assisted hydrodistillation (MHD), ultrasound‐assisted hydrodistillation (UHD) and enzyme‐assisted hydrodistillation (EHD). The chemical constituents of these EOs were analysed by gas chromatography (GC‐FID) and gas chromatography/mass spectrometry (GC/MS). Phenylpropanoids were the main components in which safrole is the dominant constituent (82.98‐91.53%), followed by monoterpene hydrocarbons (5.26‐11.94%). In comparison with traditional hydrodistillation method (HD only), the EOs obtained from the MHD, UHD and EHD methods have the higher safrole contents (91.32%, 90.96% and 90.25%, respectively). The essential oils were also tested for their antioxidant, anti‐imflammatory, antimicrobial and cytotoxic activities. The HD essential oil sample showed inhibitory activity against 6/8 tested microorganisms, i.e., Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtillis, Candida albicans, and Saccharomyces cerevisiae. The MHD and EHD exhibited the inhibitory activity against three strains E. coli, A. niger and F. oxysporum. The UHD inhibited E. coli and F. oxysporum.

Copaifera mildbraedii Desf.: Phytochemical Composition of Extracts, Essential Oil, and In Vitro Biological Activities of Bark

“…According to the literature, no studies on the identification of C. mildbraedii bark essential oil components have been published. The components of the essential oil were identified by comparing their MS patterns and retention index (RI) with those of known chemicals published in the literature [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ]. The chemical composition of the essential oil of C. mildbraedii bark was quantified using GC-FID and identified via GC-MS. Table 5 shows 30 compounds identified, representing 65% of all compounds detected in the GC-MS investigation of the essential oil, with 9 sesquiterpene hydrocarbons (26%), 7 oxygenated sesquiterpenes (22%) and 14 other compounds (17%).…”