Chemical Constituents Antioxidant and Anticholinesterasic Activity of Tabernaemontana catharinensis

Abstract

The present work aimed to analyze the alkaloid content of the ethanolic extract of Tabernaemontana catharinensis (Apocynaceae family) and its fractions as well as to evaluate their antioxidant and anticholinesterasic activities. The analyses of the ethanolic extract of T. catharinensis by mass spectrometry allowed identifying the presence of the alkaloids 16-epi-affinine, coronaridine-hydroxyindolenine, voachalotine, voacristine-hydroxyindolenine, and 12-methoxy-n-methyl-voachalotine, as well as an alkaloid with m/z 385.21 whose spectrum suggests a derivative of voacristine or voacangine. The extract and its alkaloid rich fractions showed antioxidant activity, especially those that contain the alkaloid m/z 385.21 or 16-epi-affinine with DPPH scavenging activity (IC50) between 37.18 and 74.69 μg/mL. Moreover, the extract and its fractions exhibited anticholinesterasic activity, particularly the fractions characterized by the presence of 12-methoxy-n-methyl-voachalotine, with IC50 = 2.1 to 2.5 μg/mL. Fractions with 16-epi-affinine combined good antioxidant (IC50 = 65.59 to 74.69 μg/mL) and anticholinesterasic (IC50 = 7.7 to 8.3 μg/mL) activities, representing an option for further studies aimed at treating neurodegenerative diseases.

Analyzing the Phytochemical, Anti-ulcer,Anthelmentic and Antioxidant Potentials of Tabernaemontana dichotoma Roxb.ex Wall Seed Extracts

“…T. divaricata is reported to have potential antioxidant properties and anticancer properties 7,8 . Alkoloids isolated from leaves of T. corymbosa have shown anticancer properties 9 . T. catharinensis have been reported to exert anticholinesterasic activity 10 .…”

Precursor-directed Biosynthesis in Tabernaemontana catharinensis as a New Avenue for Alzheimerʼs Disease-modifying Agents

“…catharinensis A. DC. is a species found in Argentina, Paraguay, Bolivia, and Brazil [25], and its crude extract and fractions, especially those with 12-methoxy-Nb-methyl-voachalotine, showed significant cholinesterase inhibition (IC 50 = between 2.1 and 2.5 µg/mL) [26]. In 2019, the FDAʼs Center for Drug Evaluation and Research approved 9 novel drugs for neurological purposes, which represent 19 % of all drugs released, only behind anticancer drugs (11 new drugs; 23 %) [27], showing the importance of new therapeutic alternatives in the area of neurology.…”

Research on the Effect of Antioxidant Tabernaemontana catharinensis (Apocynaceae) on Erythrocytes Exposed to the Herbicide 2,4-Dichlorophenoxyacetic Acid

“…Synthetic auxins, like, for instance, 2,4-D, are selective herbicides, that is, they are used to control determined species of weeds, and generally cause some kind of hormonal disorder (Song, 2014). Nicola et al (2013) and Boligon et al (2013) proved, by tests for the determination of its total antioxidazing capacity, that the plant activity favors the generation of reactive oxygen species (ROS).…”