Chemical constituents from the rhizomes of Polygonatum sibiricum Red. and anti-inflammatory activity in RAW264.7 macrophage cells

Abstract

Chemical investigation of the rhizomes of Polygonatum sibiricum Red. led to the identification of 27 constituents. Among them, a total of 16 compounds were obtained from Polygonatum for the first time, in which, 3 and 4 were also firstly isolated as natural products. Anti-inflammatory activity studies on 13 isolated compounds showed that β-carboline constituents, especially compounds 1 and 2, significantly inhibited the expression of NO, TNF-α, IL-6 and IL-1β in lipopolysaccharide (LPS)-treated RAW264.7 macrophage cells. Moreover, western blotting analysis demonstrated that compound 1 significantly inhibited the expression of COX-2, iNOS and the activation of NF-κB, suggesting that β-carboline structures may play an important role in inhibition of NF-κB signaling pathway, which thereby inhibits the production of inflammatory factors. The present research may not only help further elucidation of the anti-inflammatory mechanism of P. sibiricum Red., but also provide the potential bioactive molecules for inflammatory diseases research.

Identification of Antitumor Active Constituents in Polygonatum sibiricum Flower by UPLC-Q-TOF-MSE and Network Pharmacology

“…As it contains polysaccharides, saponins, flavones, lignans, amino acids, vitamins, alkaloids, and various trace elements, P. sibiricum is considered to have high medicinal and nutritional values . As recorded in the Compendium of Materia Medica, the efficacy of various parts of P.…”

“…4 As it contains polysaccharides, saponins, flavones, lignans, amino acids, vitamins, alkaloids, and various trace elements, P. sibiricum is considered to have high medicinal and nutritional values. 5 As recorded in the Compendium of Materia Medica, the efficacy of various parts of P. sibiricum used in traditional medicine decreases in the following order: flower > fruit > rhizome. 6 Therefore, in this study, we aimed to comprehensively investigate the underutilized parts of P. sibiricum and its rhizome to increase their usage and reduce waste.…”

Main Bioactive Components and Their Biological Activities from Natural and Processed Rhizomes of Polygonum sibiricum

“…Diverse flavones [ 2 ], homoisoflavanones [ 3 ], alkaloids [ 4 ], steroid saponins [ 5 ], triterpenoid saponins [ 6 ], polysaccharides [ 7 ] and their derivatives were isolated from this species in past studies. Past studies also showed that this herb exhibited anti-aging [ 7 , 8 ], anti-inflammatory [ 9 , 10 ], anti-osteoporotic [ 11 , 12 ], immune enhancing [ 13 , 14 ], neuroprotective [ 15 ], anti-diabetic [ 16 ], anticancer [ 17 ], and sleep-promoting [ 18 ] activities.…”

Mechanochemical prepared ibuprofen-Polygonatum sibiricum polysaccharide drug delivery system for enhanced bioactivity with reduced renal injury induced by NSAIDs

“…Polygonatum sibiricum is a traditional Chinese medicine for renal protection and treating nephropathy (Bibi et al., 2016 ; Cai et al., 2019 ; Zhao et al., 2019 ; Han et al., 2020 ; Wang et al., 2020 ). It has been widely used as a functional food around all Asian countries.…”