Chemical Constituents Identified from Fruit Body of Cordyceps bassiana and Their Anti-Inflammatory Activity

Abstract

Cordyceps bassiana is one of Cordyceps species with anti-oxidative, anti-cancer, anti-inflammatory, anti-diabetic, anti-obesity, anti-angiogenic, and anti-nociceptive activities. This mushroom has recently demonstrated to have an ability to reduce 2,4-dinitrofluorobenzene-induced atopic dermatitis symptoms in NC/Nga mice. In this study, we further examined phytochemical properties of this mushroom by column chromatography and HPLC analysis. By chromatographic separation and spectroscopic analysis, 8 compounds, such as 1,9-dimethylguanine (1), adenosine (2), uridine (3), nicotinamide (4), 3-methyluracil (5), 1,7-dimethylxanthine (6), nudifloric acid (7), and mannitol (8) were identified from 6 different fractions and 4 more subfractions. Through evaluation of their anti-inflammatory activities using reporter gene assay and mRNA analysis, compound 1 was found to block luciferase activity induced by NF-κB and AP-1, suppress the mRNA levels of cyclooxygenase (COX)-2 and tumor necrosis factor (TNF)-α. Therefore, our data strongly suggests that compound 1 acts as one of major principles in Cordyceps bassiana with anti-inflammatory and anti-atopic dermatitis activities.

Beauveria bassiana: a new N6-(2-hydroxyethyl)-adenosine–producing fungus

“…The fungal species produces a plethora of secondary metabolites such as bassiacridin, beauvericin, bassianolide and bassianolone (Singh et al 2015). Additionally, C. bassiana , the teleomorph of B. bassiana , produces nucleosides, such as 1,9-dimethylauanine, adenosine, uridine, 3-methyluracil and 1,7-dimethylxanthine (Park et al 2013; Suh et al 2017). In this study, we found that B. bassiana also produce HEA.…”

Adenosine Induces EBV Lytic Reactivation through ADORA1 in EBV-Associated Gastric Carcinoma

“…It has been shown previously that the de novo purine nucleotide pathway in C. militaris can produce several nucleosides, such as guanosine, inosine, adenosine, and cordycepin [23]. Another study reported that 1,9-dimethylguanine, adenosine, uridine, nicotinamide, 3-methyluracil, 1,7-dimethylxanthine, nudifloric acid, and mannitol were isolated from CME [24]. To confirm these findings, we conducted a high-performance liquid chromatography (HPLC) assay to verify the presence of nucleosides in CME.…”

“…Fresh fruiting bodies for mycelia of C. militaris were extracted with 95% ethanol at room temperature (RT) for 3 days. The extracts were filtered, concentrated, sterilized, and dried as previously described [24]. CME was dissolved in nuclease-free dH 2 O to make a stock solution at 8 mg/mL and stored at −20 °C until use.…”

Piper cubebaL. Methanol Extract Has Anti-Inflammatory Activity Targeting Src/Syk via NF-κB Inhibition

“…High-performance liquid chromatography (HPLC) analysis was used to confirm the phytochemical characteristics of Pc-ME with standard compounds including, quercetin, luteolin, and kaempferol [12], as previously reported [13, 14].…”