Chemical constituents of Barleria prionitis and their enzyme inhibitory and free radical scavenging activities

Ata, Athar; Kalhari, Kosmulalage S.; Samarasekera, Radhika

doi:10.1016/j.phytol.2008.11.005

Cited by 46 publications

(33 citation statements)

References 19 publications

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“…Kumar et al (2013) (Sawarkar et al, 2009;Nishant et al, 2014). Kumari et al, (2015) Some of the isolated compound from the herbs to name few are, 6-hydroxyflavones, scutellarein-7-rhamnosyl glucoside, barlerin, acetyl barlerin, luteolin-7-O-β-Dglucoside, shanziside methyl ester, 6-O-trans-p-coumaroyl-8-O-acetyl shanziside methyl ester, lupilinoside7-methoxydiderroside, 1,8 dihydroxy-2,7-dimethyl-3,6-dimethoxy anthraquinone,1,3,6,8-tetramethoxy-2,7-dimethyl anthraquinone, prioniside A, prioniside B, prioniside C, balarenone, pipataline (Taneja and Tiwari, 1975;Soren et al, 1982;Gupta and Saxena;1984;Chen et al, 1998;Singh et al, 2005;Ata et al, 2009;Ganga Raju et al, 2002;Ata et al, 2007;Kosmulalage et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and cytotoxic activities of Barleria prionitis and Barleria grandiflora: A comparative study

Sawarkar¹,

Kashyap²,

Pandey³

et al. 2016

Bangladesh J Pharmacol

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Leaf juices as well as leaves of Barleria prionitis and Barleria grandiflora are being used by rural people across various regions of India in the treatment of oral ailments such as dental troubles, gum ailments, pyorrhoea, dental carries and mouth ulcers. Zone of inhibition and MIC values obtained for all the extracts suggest ethanolic extract of the herbs were more antimicrobial when compared to the aqueous extract. Results of biofilm suppression were found statistically significant (p<0.05) when compared to control. Cytotoxicity was evaluated by MTT assay on human gingival fibroblast and human dermal fibroblast cell lines for ethanolic extract of the herbs. CTC50 value was found to be more than 1,000 µg/mL for ethanolic extracts of both herbs. Chlorhexidine was found to be more cytotoxic with CTC50 value of 12.5–25 µg/mL. Ethanolic extract of B. prionitis and B. grandiflora found significantly cytotoxic (p<0.05) in comparison with control.Video Clip:<a href="https://youtube.com/v/DNByJJQT3RY">Cytotoxicity assay:</a> 18 min 45 sec

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Section: Discussionmentioning

confidence: 99%

Antimicrobial and cytotoxic activities of Barleria prionitis and Barleria grandiflora: A comparative study

Sawarkar¹,

Kashyap²,

Pandey³

et al. 2016

Bangladesh J Pharmacol

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“…14, [46][47][48][49][50][51][52] Barleria grandiflora has been reported to have phytochemicals like glycosides, anthraquonone, saponins, flavanoids and phenolic compounds. [28][29][30] It will be interesting to relate the current findings of the herbs to the phytoconstituents for which further study is ongoing.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and TNF-alpha Inhibitory Activity of Barleria prionitis and Barleria grandiflora: A Comparative Study

Sawarkar¹,

Kashyap²,

Kaur³

et al. 2016

IJPER

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Background: Leaf juice as well as leaves of Barleria prionitis Linn and Barleria grandiflora Dalz (Acanthaceae) is used by rural people across various regions of India in treatment of oral ailments such as dental troubles, gum ailments, pyorrhoea, dental carries and mouth ulcers. Methods: An antimicrobial activity was carried on aqueous and ethanolic extracts of both herbs and compared with standard chlorhexidine. Cell line study was carried using Human Gingival Fibroblast on ethanolic extracts of leaves of both the herbs and compared with standard marketed preparation Zyrtee. Cytotoxicity and anti gingivitis activity were evaluated using MTT assay. Results of cytokine secretion assay and cytokine measurement assay were analyzed using ANOVA followed by Dunnett multiple comparison test. P values less than 0.05 were considered as significant. Results: Results of antimicrobial activity suggest significant potency for ethanolic extracts of both the herbs. CTC50 values for ethanolic extracts was found to be 848 µg/ml and more than 1000 µg/ml respectively for Barleria prionitis and Barleria grandiflora. The cytokine secretion significantly increased by S. mutans control, whereas the cytokine level was reduced in a dose -dependent manner by ethanolic extracts in Streotococcus mutans stimulated HGF cells. The ethanolic extracts EBG and EBP showed 65.31383 ± 1.087%, 75.0425 ± 1.268% inhibition of TNF-α at tested concentration respectively. Conclusion: Thus based on findings of this study, we conclude the usefulness of Barleria prionitis and Barleria grandiflora in treatment of oral ailments such as gingivitis.

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“…The leaves of B. prionitis L. were found to contain scutellarein, melilotic acid, syringic acid, vanillic acid, p-hydroxybenzoic acid, and 6-hydroxyflavones 12 . From the aerial parts of Barleria prionitis, phenylethanoid glycoside, barlerinoside along with six iridoid glycosides, shanzhiside methyl ester, 6-O-trans-p-coumaroyl-8-O-acetylshanzhiside methyl ester, barlerin, acetylbarlerin, 7-methoxydiderroside, and lupulinoside were isolated and structures of these compounds were elucidated with the aid of extensive NMR spectral studies and chemical reactions 13 .…”

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confidence: 99%

“…are generated from various biological reactions in the body. These ROS can cause cellular injuries, DNA damage and mutations 7,8,9,13,15,16,17 . Medicinal plants are important source of a number of antioxidants, mainly polyphenols.…”

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“…Medicinal plants are important source of a number of antioxidants, mainly polyphenols. Recently, in vitro studies have been undertaken in B. prionitis L. for determination of antioxidant properties and free radical scavenging activity 8,9,13,15,16,17 . However, the information regarding effects of various factors such as plant parts, solvents used and method of extraction on phytochemical content and antioxidant activity is very limited.…”

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2016

IJPSR

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ABSTRACT:In the present investigation phenolic contents and antioxidant potential of Barleria prionitis L. leaf and stem extracts were analyzed by in vitro antioxidant assays and RP-HPLC method. The results indicated that high amount of total phenolics were present in acetone extracts of leaf (20.22±0.57 mg of GAE/g) and stem (21.19±1.25 mg of GAE/g). Highest flavonoid content was observed in ethanolic (44.23±2.82 mg of RE/g) and acetone extracts (44.94±0.85 mg of RE/g) of leaf, while stem extracts were poor in flavonoid contents. RP-HPLC method for determination of specific phenolic compounds was reported for the first time in B. prionitis L. Out of the phenolic compounds tested, Gallic acid was most common and more abundantly present in both leaf and stem of B. prionitis L. Aqueous extract of leaf showed highest extraction capacity for Gallic acid (211.70 μg/g) and Catechol (112.57 μg/g), while Caffeic acid (37.74 μg/g) and p-Coumaric acid (48.66 μg/g) were highest in methanolic extract. The present study indicated strong influence of extraction solvents and plant parts on antioxidant potential of plant extracts as well as quantity and quality of phytocompounds. The stem of B. prionitis L. plants was found to be superior in phenolics and antioxidant potential. RP-HPLC results however noted higher amount and number of phenolic compounds in leaf than stem extracts, indicating presence of other phenolic compounds in stem. Out of the four extraction solvents tested, acetone was best while water was found to be weakest in terms of extraction capacity.

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Chemical constituents of Barleria prionitis and their enzyme inhibitory and free radical scavenging activities

Cited by 46 publications

References 19 publications

Antimicrobial and cytotoxic activities of <i>Barleria prionitis</i> and <i>Barleria grandiflora</i>: A comparative study

Antimicrobial and cytotoxic activities of <i>Barleria prionitis</i> and <i>Barleria grandiflora</i>: A comparative study

Antimicrobial and TNF-alpha Inhibitory Activity of Barleria prionitis and Barleria grandiflora: A Comparative Study

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