Chemical Constituents of Thesium chinense Turcz and Their In Vitro Antioxidant, Anti-Inflammatory and Cytotoxic Activities

Abstract

Three novel compounds (1–3) along with twenty-six known compounds, two known steroids (4–5) and twenty-four known phenylpropanoids (6–29) were isolated from the whole plant of Thesium chinense Turcz. The structures of the three new compounds were elucidated on the basis of ESI-MS, HR-ESIMS, 1D and 2D NMR, IR, UV spectroscopic data. The absolute stereochemistry of compound 1 was determined by the Gauge-Including Atomic Orbitals (GIAO) method. The in vitro antioxidant, anti-inflammatory and cytotoxic activities of the isolated compounds were evaluated by DPPH radical-scavenging assay, LPS-activated RAW 264.7 cells model and CCK-8 kit, respectively. Compound 11 showed high antioxidant activity with an SC50 value of 16.2 ± 1.6 μM. Compound 21 showed considerable anti-inflammatory activity with an IC50 value of 28.6 ± 3.0 μM. Compounds 4 and 5 displayed potent cytotoxic activity against human NCI-H292, SiHa, A549, and MKN45 cell lines, with the compound 4 having IC50 values of 17.4 ± 2.4, 22.2 ± 1.1, 9.7 ± 0.9, 9.5 ±0.7 μM, and the compound 5 having all IC50 values less than 0.1 μM in vitro.

Systematic Screening of the Chemical Constituents of Lanqin Oral Liquid by Ultra-High-Performance Liquid Chromatography Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

“…Organic acids, for example, caffeic acid and chlorogenic acid, show antioxidant, antibacterial, and anti-inflammatory activities [40,41]. Phenylpropanoids, for example, esculin, shows anti-inflammatory and antioxidative stress effects [42]; pinoresinol-4-O-glucoside shows antioxidant activity [43]; and lariciresinol-4-O-β-D-glucopyranoside shows anti-inflammatory and antiviral effects [44]. It can also be assumed that not only the main chemical constituents with high content but also all of these compounds identified in the Lanqin oral liquid have the probability of being bioactive constituents.…”

Systematic investigation of the material basis, effectiveness and safety of Thesium chinense Turcz. and its preparation Bairui Granules against lung inflammation

“…Phytochemical and pharmacological aspects of T. chinense has not been extensively studied. Hitherto, only about 60 compounds including flavonoids, alkaloids, phenylpropanoids, steroids, and fatty acids, have been identified, and flavonoids are recognized as the main chemical constituents of T. chinense [ 9 – 15 ]. Pharmacological test indicated that T. chinense inhibited mouse auricle swelling caused by xylene, rat foot swelling caused by egg white, rat cotton ball pulp odontoma, and mice’s ear edema caused by xylene [ 16 , 17 ].…”

Exploring Information Exchange between Thesium chinense and Its Host Prunella vulgaris through Joint Transcriptomic and Metabolomic Analysis

“…Thesium chinense, a hemiparasitic plant within the genus Thesium of the Santalaceae family, exhibits a widespread distribution across Africa, Europe, Asia and America [6]. Contemporary pharmacological investigations have proved that T. chinense possesses antiinflammation properties [7,8], antimicrobial effect [9][10][11], analgesic activity [12], antioxidant activity [13] and anti-nephropathy [14]. Termed as a "plant antibiotic" [15], T. chinense demonstrates therapeutic potential in addressing various conditions including mastitis, tonsillitis, pharyngitis, pneumonia, and upper respiratory tract infections [9,16,17].…”