Chemical Constituents of Tibetan Herbal Medicine Pulicaria insignis and Their in vitro Cytotoxic Activities

Wang, Xinzhu; Hou, Peilei; Qu, Yanbo; Huang, Ri-Zhen; Yang, Feng; Zhao, Su; Ding, Yelin; Liao, Zhi‐Xin

doi:10.25135/rnp.193.20.06.1692

Cited by 5 publications

(7 citation statements)

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“…Previously, MTT assay was used to evaluate the cytotoxic effects of the 19 purified compounds isolated from the Pulicaria insignis against four human cancer cell lines, including HeLa. The best results against HeLa cells were obtained with flavonoids and sesquiterpene lactone [20]. Pulicaria undulata aerial parts extract was also evaluated for cytotoxicity against breast and hepatoma cancer.…”

Section: Results Of Cytotoxicity Testingmentioning

confidence: 99%

“…After 2 hours of incubation at room temperature (20°C ≤ t ≤ 25°C), the absorbance was measured at 765 nm. Quantitative measurements were performed using a standard calibration curve of different concentrations of gallic acid (20,100,200, 300, 400 and 500 mg/L) in the same way. The results for TPC are expressed in galic acid equivalents (GAE) as miligram per gram of dry extract (mg GAE/g).…”

Section: Determination Of Total Phenolic Content (Tpc)mentioning

confidence: 99%

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Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts

Kozarević

Dautović²,

Halilčević³

et al. 2022

IRJPAC

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Aims: The aims of the study were to analyse the polyphenols of Pulicaria dysenterica (L.) Bernh. methanolic extracts from aerial and underground parts, assessment of antioxidant activity and to evaluate their cytotoxicity on HeLa cells of cervical cancer. Methodology: The total phenolic content (TPC) of extracts was determined by the Folin-Ciocalteu spectrophotometric method. The qualitative and quantitative analysis of individual polyphenolic compounds were performed by the reverse phase HPLC method. The antioxidant capacity was evaluated by both, 2,2-diphenyl1-picrylhydrazyl radical and FRAP assay, while cytotoxicity of the extracts was assessed by MTT assay. Results: TPC of the samples were 127.62±2.22 and 244.12±8.84 mg gallic acid equivalent/g extract. In the extracts chlorogenic acid in amount of 10.06±0.96 and 11.32±0.28 mg/g, flavonoid rutin in amount of 5.68±0.13 mg/g and three caffeic acid derivatives were recorded. Extract from underground parts achieved better antioxidant activity with IC50 value 55.36±0.75 µg/mL and FRAP value 2411.12±37.22 µmol Fe2+g-1 compared to the one from aerial parts. Extract from aerial parts achieved better cytotoxic activity with 50% inhibition of viability (IC50) at concentration of 0.389±0.07 mg/mL, against HeLa cells, compared to the extract from underground parts. Conclusion: Analyzed Pulicaria dysenterica extracts contained phenolic acids and flavonoids. The extracts showed good antioxidant activity and cytotoxic properties against HeLa cells in vitro.

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Section: Results Of Cytotoxicity Testingmentioning

confidence: 99%

Section: Determination Of Total Phenolic Content (Tpc)mentioning

confidence: 99%

Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts

Kozarević

Dautović²,

Halilčević³

et al. 2022

IRJPAC

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“…The antiallergic activity was confirmed in vivo using a passive cutaneous anaphylaxis (PCA) reaction model in mice [294]. Nepetin from P. insignis demonstrated cytotoxic activity towards HeLa and HepG2 human cancer cell lines (IC50: 3.61-3.98 μM) but was inactive against MGC803 and T24 human cancer cells [95].…”

Section: Biological Activity Of Flavonesmentioning

confidence: 89%

“…6-Hydroxykaempferol 3,7-dimethyl ether and chrysosplenol C from P. inuloides were moderately (IC50: 16.8-19.6 μg/mL) but selectively active against prostate cancer cell line (PC3) resistant to doxorubicin [184]. 6-Methoxykaempferol 3,4'-dimethyl ether (santin), isolated from the inflorescences of P. insignis, exerted cytotoxic effect on the MGC-803, HeLa, Hep G2, and T24 human cancer cell lines (IC50: 3.71-4.78 μM) whereas the corresponding IC50 values for 6-methoxykaempferol 3-methyl ether and 6-methoxyquercetin 3-methyl ether, isolated from the same plant material, were higher than 40 μM [95].…”

Section: Biological Activity Of Flavonolsmentioning

confidence: 99%

Polyphenols of the Inuleae-Inulinae and Their Biological Activities: A Review

Malarz,

Michalska,

Stojakowska

2024

Preprint

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Polyphenols are ubiquitous plant metabolites that demonstrate biological activities essential to the plant-environment interactions. They are of interest to plant food consumers, as well as to the food, pharmaceutical and cosmetic industry. The class of the plant metabolites comprises both widespread (chlorogenic acids, luteolin, quercetin) and unique compounds of diverse chemical structures but of the common biosynthetic origin. Polyphenols, next to sesquiterpenoids are regarded as the major class of the Inuleae-Inulinae metabolites responsible for the pharmacological activity of medicinal plants from the subtribe (Blumea spp., Dittrichia spp., Inula spp., Pulicaria spp. and others). Recent decades have brought a rapid development of molecular and analytical techniques which resulted in better understanding of the taxonomic relationships within the Inuleae tribe and in a plethora of data concerning the chemical constituents of the Inuleae-Inulinae. The current taxonomical classification has introduced changes in the well-established botanical names and rearranged the genera based on molecular plant genetic studies. The newly created chemical data together with the earlier phytochemical studies may provide some complementary information on biochemical relationships within the subtribe. Moreover, they may at least partly explain pharmacological activities of the plant preparations traditionally used in therapy. The current review aimed to systematize the knowledge on the polyphenols of the Inulae-Inulinae.

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“…(Asteraceae, Inuleae-Inulinae) comprises over 80 species that are native to Europe, Asia, and Africa [ 1 ]. Only about one-third of them have been phytochemically investigated so far [ 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. Numerous species are endemic to the Mediterranean Basin, and only two: P. dysenterica (L.) Bernh.…”

Section: Introductionmentioning

confidence: 99%

Constituents of Pulicaria inuloides and Cytotoxic Activities of Two Methoxylated Flavonols

et al. 2023

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Plants of the genus Pulicaria are known for providing traditional medicines, spices, herbal teas, and insect deterrents. Pulicaria inuloides (Poir.). DC. is one of the less chemically studied species within the genus. Hydroalcoholic extracts from roots and aerial parts of P. inuloides were analyzed using the UHPLC-PAD-MSn technique and revealed the presence of six caffeoylquinic and eleven caffeoylhexaric conjugates together with hydroxykaempferol dimethyl ether and quercetagetin trimethyl ether. Moreover, constituents of chloroform extract from the whole P. inuloides plants were isolated and identified by spectroscopic methods. One new and four known caryophyllene derivatives, three thymol derivatives, and four polymethoxylated flavonols were found in the analyzed extract. The structure of the new compound was established by spectroscopic methods (HRESIMS, 1H NMR, 13C NMR, COSY, HSQC, HMBC, NOESY). The cytotoxicity of 6-Hydroxykaempferol 3,7-dimethyl ether and quercetagetin 3,7,3’-trimethyl ether (chrysosplenol C), which are major flavonols isolated from the plant, were tested on prostate epithelial cells (PNT2), prostate cancer cells (DU145 and PC3), human keratinocytes (HaCaT), and melanoma cells (HTB140 and A375). Both flavonols demonstrated moderate cytotoxic activity against PC3 cells (IC50 = 59.5 µM and 46.6 µM, respectively). The remaining cell lines were less affected (IC50 > 150 µM).

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Chemical Constituents of Tibetan Herbal Medicine Pulicaria insignis and Their in vitro Cytotoxic Activities

Cited by 5 publications

References 0 publications

Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts

Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts

Polyphenols of the Inuleae-Inulinae and Their Biological Activities: A Review

Constituents of Pulicaria inuloides and Cytotoxic Activities of Two Methoxylated Flavonols

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