Chemical conversion of human epidermal stem cells into intestinal goblet cells for modeling mucus-microbe interaction and therapy

Zhao, Andong; Qin, Hua; Sun, Mengli; Tang, Mao; Mei, Jinyu; Ma, Kui; Fu, Xiaobing

doi:10.1126/sciadv.abb2213

Cited by 17 publications

(12 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several transcription factors, inflammatory cytokines and gut microbes have been implicated in the modulation of goblet cell differentiation. 38,39 ATOH1 (also called Math1), a Notch target gene, is involved in the differentiation of secretory lineages in the intestine. 40 Transcription factor SPDEF, previously thought to regulate goblet cell hyperplasia in the lung, was found to work downstream of ATOH1 to define differentiation toward secretory cell lineages, and its deficiency caused a severely impaired maturation of goblet and Paneth cells and an accumulation of immature secretory progenitors.…”

Section: Epa Promoted Mucin Production By Enhancing Goblet Cell Diffe...mentioning

confidence: 99%

EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier

et al. 2022

View full text Add to dashboard Cite

show abstract

Section: Epa Promoted Mucin Production By Enhancing Goblet Cell Diffe...mentioning

confidence: 99%

EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The observed changes in the number of mucus cells and activities of antioxidant enzymes in this study may have resulted from changes in intestinal microbiota that occurred under transport stress. Further studies using mucinous cell and goblet cell models would be useful to clarify the function of different types of mucinous cells and their changes under stress conditions [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Integrated Transcriptome and 16S rDNA Analyses Reveal That Transport Stress Induces Oxidative Stress and Immune and Metabolic Disorders in the Intestine of Hybrid Yellow Catfish (Tachysurus fulvidraco♀ × Pseudobagrus vachellii♂)

Zheng

Tao

et al. 2022

Antioxidants

View full text Add to dashboard Cite

Live fish are often transported in aquaculture. To explore the effects of transport stress, hybrid yellow catfish (Tachysurus fulvidraco♀ × Pseudobagrus vachellii♂) were subjected to simulated transport treatments (0–16 h) with 96 h of recovery after the 16-h transport treatment, and intestinal biochemical parameters, the transcriptome, and gut microbiota were analyzed. Transportation affected the number of mucus cells and led to oxidative stress in the intestine, which activated immune responses. Changes in lipid metabolism reflected metabolic adaptation to oxidative stress. Toll-like receptor signaling, peroxisome proliferator-activated receptor signaling, and steroid biosynthesis pathways were involved in the transport stress response. Gene expression analyses indicated that transport-induced local immune damage was reversible, whereas disordered metabolism recovered more slowly. A 16S rDNA analysis revealed that transport stress decreased the alpha diversity of the gut microbiota and disrupted its homeostasis. The dominant phyla (Fusobacteria, Bacteroidetes) and genera (Cetobacterium, Barnesiellaceae) were involved in the antioxidant, immune, and metabolic responses of the host to transportation stress. Correlation analyses suggested that gut microbes participate in the transport stress response and the host–microbiota interaction may trigger multiple events in antioxidant, immune, and metabolic pathways. Our results will be useful for optimizing transport processes.

show abstract

“…The intestinal mucus layer is one crucial part of the gut barrier to preventing foreign invasion [16]. The mucins were synthesized, stored and secreted by goblet cells in the gut of animals [29]. When the mucus layer was defective, numerous bacteria could invade the host and cause serious immune response [17] and inflammatory bowel disease [30].…”

Section: Discussionmentioning

confidence: 99%

Sub-Chronic Difenoconazole Exposure Induced Gut Microbiota Dysbiosis in Mice

Zhaorigetu

Wang

et al. 2022

Toxics

View full text Add to dashboard Cite

Difenoconazole (DIF) is a widely separated triazole fungicide in many countries. The excessive usage of DIF increases the high volume of residues in agriculture production and water bodies. Some previous studies demonstrated the toxic effects of DIF on non-target animals, however, there were still some gaps in the knowledge of the potential hazards of DIF to mammals and human health. Herein, 7-week-old male mice were exposed to 30 and 100 mg/kg/day DIF for 14 and 56 days. We observed that 56 days of DIF exposure decreased the colonic mucus expression of alcin blue-periodic acid-schiff (AB-PAS) stain and the immunochemical stain of muc2 protein. The transcript levels of mucin protein (muc1, muc2 and muc3) decreased significantly in the gut of mice followed 56 days of 100 mg/kg/day DIF exposure. In addition, the gut microbiota composition was also affected after 14 or 56 days of DIF exposure. Although the mucus expression after 14 days of DIF exposure only decreased slightly, the gut microbiota composition compared with the control group was changed significantly. Moreover, the DIF-30 and DIF-100 caused respectively different changes on the gut microbiota. The relative abundance of Bacteroidetes decreased significantly after 14 days and 56 days of DIF exposure. After 14 days of DIF exposure, there were 35 and 18 differential genera in the DIF-30 and DIF-100 group, respectively. There were 25 and 32 differential genera in the DIF-30 and DIF-100 group after 56 days of exposure, respectively. Meanwhile, the alpha diversity indexes, including observed species, Shannon, Simpson, Chao1 and ACE, in gut microbiota decreased significantly after 56 days of DIF exposure. Interestingly, the relative abundance of Akkermansia increased significantly after 56 days of 100 mg/kg/d DIF exposure. Although Akkermansia was considered as one probiotic, the phenomenon of dramatic Akkermansia increase with the decrease in gut microbiota diversity needed further discussion. These results provided some new insights on how DIF exposure impacts the mucus barrier and induces gut microbiota dysbiosis.

show abstract

Chemical conversion of human epidermal stem cells into intestinal goblet cells for modeling mucus-microbe interaction and therapy

Cited by 17 publications

References 57 publications

EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier

EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier

Integrated Transcriptome and 16S rDNA Analyses Reveal That Transport Stress Induces Oxidative Stress and Immune and Metabolic Disorders in the Intestine of Hybrid Yellow Catfish (Tachysurus fulvidraco♀ × Pseudobagrus vachellii♂)

Sub-Chronic Difenoconazole Exposure Induced Gut Microbiota Dysbiosis in Mice

Contact Info

Product

Resources

About