Chemical cross-linking on gelatin-hyaluronan loaded with hinokitiol for the preparation of guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties

Chang, Kai‐Chi; Chen, Wen‐Cheng; Chen, Chih-Hua; Shih, Chi‐Jen; Liu, Shih-Ming; Chen, Jian-Chih

doi:10.1016/j.msec.2020.111576

Cited by 30 publications

(19 citation statements)

References 38 publications

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“…Therefore, with the addition of modified DCPA, the ion–dipole physical bond between Ca 2+ and gelatin increases [ 24 ] ( Figure 1 b). However, hinokitiol with a hydroxyl and an isopropyl group leads to a large dipole moment [ 25 , 26 ]. Once hinokitiol is distributed in the hydrogel membrane, its hydrophobicity tends to cap the surface of hyaluronic acid instead of gelatin, and it interacts with Ca 2+ at the same time, thereby reducing the network structure of the ion–dipole physical bond between Ca 2+ and gelatin ( Figure 1 c).…”

Section: Resultsmentioning

confidence: 99%

“…After D1 was cultured on the surfaces for 7 days, the group with the addition of modified DCPA, namely, Ec-0.5D, had the greatest proliferation effect. The cell proliferation capability in all of the hydrogels impregnated with hinokitiol was significantly slowed down, possibly because hinokitiol has an antibacterial effect that inhibits cell proliferation [ 26 , 28 ]. The ALP activity produced by the D1 cells after membrane contact culture is shown in Figure 11 b.…”

Section: Resultsmentioning

confidence: 99%

“…Research has shown that the RGD sequence can promote cell adhesion in tissue engineering applications [ 25 , 26 ]. Resorbable bioceramics are largely incorporated into biodegradable polymer blends used in bone tissue engineering applications [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…The hydrogel was further combined with the hinokitiol group of Ec-0.5D-2H, and it showed an obvious proliferation capability and delayed ALP production after 10 days of culture. This phenomenon is speculated to be due to the release of hinokitiol during culture, which affects cell proliferation and ALP production [ 26 ]. According to studies, the use of osteogenic factors is not effective for bone tissue regeneration before eliminating bacterial infections and inflammatory symptoms; thus, bacterial infections and inflammatory symptoms must be controlled before assisting osteoregeneration [ 4 , 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

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Effects of Hinokitiol and Dicalcium Phosphate on the Osteoconduction and Antibacterial Activity of Gelatin-Hyaluronic Acid Crosslinked Hydrogel Membrane In Vitro

et al. 2021

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Many hydrogel-based crosslinking membranes have been designed and tailored to meet the needs of different applications. The aim of this research is to design a bifunctional hydrogel membrane with antibacterial and osteoconducting properties to guide different tissues. The membrane uses gelatin and hyaluronic acid as the main structure, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride as the crosslinker, hinokitiol as the antibacterial agent, and dicalcium phosphate anhydrous (DCPA) micron particles for osteoconduction. Results show that the hydrogel membrane with added DCPA and impregnated hinokitiol has a fixation index higher than 88%. When only a small amount of DCPA is added, the tensile strength does not decrease significantly. The tensile strength decreases considerably when a large amount of modified DCPA is added. The stress–strain curve shows that the presence of a large amount of hinokitiol in hydrogel membranes results in considerably improved deformation and toughness properties. Each group impregnated with hinokitiol exhibits obvious antibacterial capabilities. Furthermore, the addition of DCPA and impregnation with hinokitiol does not exert cytotoxicity on cells in vitro, indicating that the designed amount of DCPA and hinokitiol in this study is appropriate. After a 14-day cell culture, the hydrogel membrane still maintains a good shape because the cells adhere and proliferate well, thus delaying degradation. In addition, the hydrogel containing a small amount of DCPA has the best cell mineralization effect. The developed hydrogel has a certain degree of flexibility, degradability, and bifunctionality and is superficial. It can be used in guided tissue regeneration in clinical surgery.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Hinokitiol and Dicalcium Phosphate on the Osteoconduction and Antibacterial Activity of Gelatin-Hyaluronic Acid Crosslinked Hydrogel Membrane In Vitro

et al. 2021

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“…Compared with the control group, the inhibitory effect on E. coli growth was more important than that on S. aureus growth as the amount of hinokitiol loading increased [30]. Indeed, the hydrogel regeneration membranes crosslinking by N-(3-dimethylaminopropyl)-Nethylcarbodiimide hydrochloride had the largest inhibition area against S. aureus and E. coli when it is impregnated with a high concentration of hinokitiol [31].…”

Section: Antibacterial Effects and Mechanismsmentioning

confidence: 94%

Health Benefits and Pharmacological Properties of Hinokitiol

et al. 2021

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Hinokitiol is a natural bioactive compound found in several aromatic and medicinal plants. It is a terpenoid synthetized and secreted by different species as secondary metabolites. This volatile compound was tested and explored for its different biological properties. In this review, we report the pharmacological properties of hinokitiol by focusing mainly on its anticancer mechanisms. Indeed, it can block cell transformation at different levels by its action on the cell cycle, apoptosis, autophagy via inhibiting gene expression and dysregulating cellular signaling pathways. Moreover, hinokitiol also exhibits other pharmacological properties, including antidiabetic, anti-inflammatory, and antimicrobial effects. It showed multiple and several effects through its inhibition, interaction and/or activation of the main cellular targets inducing these pathologies.

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CSMP: A Self‐Assembled Plant Polysaccharide‐Based Hydrofilm for Enhanced Wound Healing

Qiao,

Zhang,

Liu

et al. 2023

Adv Healthcare Materials

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Wound management remains a critical healthcare issue due to the rising incidence of chronic diseases leading to persistent wounds. Traditional dressings have their limitations, such as potential for further damage during changing and suboptimal healing conditions. Recently, hydrogel‐based dressings have gained attention due to their biocompatibility, biodegradability, and ability to fill wounds. Particularly, polysaccharide‐based hydrogels have shown potential in various medical applications. This study focuses on the development of a novel hydrofilm wound dressing produced from a blend of chia seed mucilage (CSM) and polyvinyl alcohol (PVA), termed CSMP. While the individual properties of CSM and PVA are well‐documented, their combined potential in wound management is largely unexplored. CSMP, coupled with sorbitol and glycerin, and cross‐linked using ultraviolet light, results in a flexible, adhesive, and biocompatible hydrofilm demonstrating superior water absorption, moisturizing, and antibacterial properties. This hydrofilm promotes epithelial cell migration, enhanced collagen production, and outperforms existing commercial dressings in animal tests. The innovative CSMP hydrofilm offers a promising, cost‐effective approach for improved wound care, bridging existing gaps in dressing performance and preparation simplicity. Future research can unlock further applications of such polysaccharide‐based hydrofilm dressings.

show abstract

Chemical cross-linking on gelatin-hyaluronan loaded with hinokitiol for the preparation of guided tissue regeneration hydrogel membranes with antibacterial and biocompatible properties

Cited by 30 publications

References 38 publications

Effects of Hinokitiol and Dicalcium Phosphate on the Osteoconduction and Antibacterial Activity of Gelatin-Hyaluronic Acid Crosslinked Hydrogel Membrane In Vitro

Effects of Hinokitiol and Dicalcium Phosphate on the Osteoconduction and Antibacterial Activity of Gelatin-Hyaluronic Acid Crosslinked Hydrogel Membrane In Vitro

Health Benefits and Pharmacological Properties of Hinokitiol

CSMP: A Self‐Assembled Plant Polysaccharide‐Based Hydrofilm for Enhanced Wound Healing

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