Chemical Denervation with Botulinum Neurotoxin A Improves the Surgical Manipulation of the Muscle–Tendon Unit: An Experimental Study in an Animal Model

Mannava, Sandeep; Callahan, Michael F.; Trach, Simon M.; Wiggins, Walter F.; Smith, Beth Paterson; Koman, L. Andrew; Smith, Thomas L.; Tuohy, Christopher J.

doi:10.1016/j.jhsa.2010.11.014

Cited by 17 publications

(20 citation statements)

References 49 publications

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“…40 Yet in animal studies, modulation of the passive stiffness of the muscle-tendon unit, by a one-time dose of BoNT-A, seem to reverse 2 weeks after injection. 28 Our data demonstrate that BoNT-A significantly FIGURE 5. The effect of displacement of the rat supraspinatus muscle-tendon unit on passive peak and equilibrium tension.…”

Section: Discussionmentioning

confidence: 63%

Contributions of Neural Tone to In Vivo Passive Muscle–Tendon Unit Biomechanical Properties in a Rat Rotator Cuff Animal Model

et al. 2011

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Passive viscoelastic properties of muscle-tendon units are key determinants of intra- and post-operative success. Atrophied, retracted, and stiff muscle-tendon units are technically challenging to manipulate and perform poorly after surgical repair. This study employs botulinum neurotoxin A (BoNT-A)-mediated inhibition of presynaptic acetylcholine release to examine in vivo neural contributions to soft-tissue biomechanical properties. In vivo load-relaxation and active muscle contractile force testing protocols were performed in the rat rotator cuff model. The passive properties were assessed using linear regression analysis and Fung's quasi-linear viscoelastic (QLV) model. BoNT-A injected muscle--tendon units had a significant reduction in force of contraction (p = 0.001). When compared to saline injected controls, the BoNT-A significantly decreased parameter 'A' of the QLV model, which represents the linear elastic response (p = 0.032). The viscous properties in the BoNT-A treatment group were not significantly different from saline injected controls, as determined by comparison of QLV model parameters 'C,' 'τ(1),' and 'τ(2).' In conclusion, neural tone contributes significantly to muscle-tendon unit passive biomechanical properties. Pre-surgical treatment with BoNT-A may improve the rehabilitation of muscle by altering its passive elastic properties. Accordingly, pharmacological modulation of skeletal muscle stiffness with BoNT-A increases flexibility, potentially improving function. Chemical denervation with BoNT-A may also improve the manipulation of stiff and difficult to mobilize muscles during surgical procedures.

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Section: Discussionmentioning

confidence: 63%

Contributions of Neural Tone to In Vivo Passive Muscle–Tendon Unit Biomechanical Properties in a Rat Rotator Cuff Animal Model

et al. 2011

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“…[1][2][3][4][5][6] In addition, botulinum toxin is emerging as a treatment modality for Raynaud phenomenon. [7][8][9][10] The mechanism of action of BoNTA at the neuromuscular junction and the functional recovery of the nerve and muscle are well established [11][12][13][14][15][16][17][18] ; however, little information is available regarding toxin effects on the local vasculature. [7][8][9]19 Arteriolar diameter and blood flow is predominantly controlled by the sympathetic nervous system, which releases norepinephrine after stimulation and results in ␣-adrenergic receptor-mediated vasoconstriction.…”

mentioning

confidence: 99%

The Effect of Botulinum Neurotoxin-A on Blood Flow in Rats: A Potential Mechanism for Treatment of Raynaud Phenomenon

Stone

Koman

Callahan

et al. 2012

The Journal of Hand Surgery

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“…The experimental apparatus and the surgical approach were similar to previous reports [21,22]. Briefly, eight days post-injection, the saline-injected control animals ( n = 18) and BoNT-A experimental animals ( n = 18) were anesthetized with isoflurane (1.5–2.0 vol.%).…”

Section: Methodsmentioning

confidence: 99%

“…In all instances, 1.2 V was used during the active force of contraction protocol [20,21,22]. For tetanic contraction force, a square wave stimulus of 1.2 V at a frequency of 100 Hz for 1 s was used.…”

Section: Methodsmentioning

confidence: 99%

Botulinum Neurotoxin A Injections Influence Stretching of the Gastrocnemius Muscle-Tendon Unit in an Animal Model

Haubruck

Mannava

Plate

et al. 2012

Toxins

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Botulinum Neurotoxin A (BoNT-A) injections have been used for the treatment of muscle contractures and spasticity. This study assessed the influence of (BoNT-A) injections on passive biomechanical properties of the muscle-tendon unit. Mousegastrocnemius muscle (GC) was injected with BoNT-A (n = 18) or normal saline (n = 18) and passive, non-destructive, in vivo load relaxation experimentation was performed to examine how the muscle-tendon unit behaves after chemical denervation with BoNT-A. Injection of BoNT-A impaired passive muscle recovery (15% vs. 35% recovery to pre-stretching baseline, p < 0.05) and decreased GC stiffness (0.531 ± 0.061 N/mm vs. 0.780 ± 0.037 N/mm, p < 0.05) compared to saline controls. The successful use of BoNT-A injections as an adjunct to physical therapy may be in part attributed to the disruption of the stretch reflex; thereby modulating in vivo passive muscle properties. However, it is also possible that BoNT-A injection may alter the structure of skeletal muscle; thus modulating the in vivo passive biomechanical properties of the muscle-tendon unit.

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Chemical Denervation with Botulinum Neurotoxin A Improves the Surgical Manipulation of the Muscle–Tendon Unit: An Experimental Study in an Animal Model

Cited by 17 publications

References 49 publications

Contributions of Neural Tone to In Vivo Passive Muscle–Tendon Unit Biomechanical Properties in a Rat Rotator Cuff Animal Model

Contributions of Neural Tone to In Vivo Passive Muscle–Tendon Unit Biomechanical Properties in a Rat Rotator Cuff Animal Model

The Effect of Botulinum Neurotoxin-A on Blood Flow in Rats: A Potential Mechanism for Treatment of Raynaud Phenomenon

Botulinum Neurotoxin A Injections Influence Stretching of the Gastrocnemius Muscle-Tendon Unit in an Animal Model

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