Chemical Effects of γ-Irradiation of Aqueous Solutions of Heparin and Keratan Sulphate

Chemical Effects of γ-irradiation of Aqueous Solutions of Chondroitin-4-sulphate

“…The yields of these products are extremely small, though this is not surprising in view of their low yield from irradiated glucuronic acid and N-acetyl glucosamine (Scherz 1970) . Furthermore, no sulphate is released and this is consistent with the studies on y-irradiated heparin and keratan sulphate (Jooyandeh et al 1971) and also on y-irradiated D-glucose 3-sulphate and 2-deoxy 2-sulphamino D-glucose (Bain 1970) .…”

“…Irradiations were carried out using a 60Co y-irradiation source to final doses varying from 0-2 x 10 19 eV ml -1 . Full details have been previously described (Jooyandeh et al 1971) .…”

“…The hydrated electron, on the other hand, reacts extremely slowly with these components of connective tissue (Moore et al . 1970) and appears to play no role in the formation of the permanent products obtained after 60 Co y-irradiation of heparin and keratan sulphate (Jooyandeh, Moore, Morgan and Phillips 1971) . In the present study, analysis of the products after y-radiolysis of aqueous solutions of chondroitin 4-sulphate indicates that it is the hydroxyl radical which is responsible for the chemical changes.…”

Chemical Modifications, Solid Phase, Radio‐Chemical and Enzymatic Transformations of Hyaluronic Acid

“…The radical OH* could attack all carbon atoms in the carbohydrate cycle, to which hydroxyl groups are attached; or, carbon atoms involved into glycosidic bonds. Similar rate constants of the OH* reaction and hydrated electron with heparin, keratan sulfate and carboxymethylcellulose were presented in [72]. As a result of such attack the polysaccharide macroradicals of different structures are formed [66].…”