1974
DOI: 10.1016/0014-5793(74)80096-7
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Chemical evidence for associated TRF with subcellular fractions after incubation of intact rat prolactin cells (GH3) with 3H‐labelled TRF

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Cited by 30 publications
(15 citation statements)
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References 11 publications
(12 reference statements)
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“…First of all, the present experiments confirm our previous findings [Brunet et al, 1974] that within 30 min of incubation with target cells there is no significant degradation of bound TRH. They also show that no TRH degradation occurred at the same time in the medium.…”
Section: Discussionsupporting
confidence: 81%
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“…First of all, the present experiments confirm our previous findings [Brunet et al, 1974] that within 30 min of incubation with target cells there is no significant degradation of bound TRH. They also show that no TRH degradation occurred at the same time in the medium.…”
Section: Discussionsupporting
confidence: 81%
“…Radioactivity was measured with an Intertechnique SL 30 scintillation spectrometer as previously described [Brunet et a!., 1974], Prolactin assay. PRL was measured by radioimmunoassay using the rat PRL kit provided by the N1AMDD Rat Hormone Distribution Program.…”
Section: Analysis O F [3h]-rmmentioning
confidence: 99%
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“…In those conditions, the amount of [3H]-TRH bound to isolated nuclei represented around 20% (table II) of that bound to intact GH3 cells incubated simultaneously with the same dose and for the same duration. This percentage is similar to that already measured when nuclei were isolated from GH3 cells previously in cubated with the same dose of [3H]-TRH and for the same duration [Brunet et al, 1974],…”
Section: Purity O F Nuclear Fractionsupporting
confidence: 71%
“…Intact GH3 cells have been shown to posses specific binding sites for [3H]-TRH [Gourdji et al, 1973; Hinkle and Tashjian, 1973], The binding is time dependent, reaching a plateau after the first [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] min, at the same lime as the TRH-induced stimulation of Prl release. Two classes of TRH binding sites have been detected on intact G H 3 cells in suspension, one of high affinity (Kr>: 4 x I0~sm) for physiological doses and the other of low affinity (3 x 10~7m for high doses (front 150 up to 1080 nM) [Tixier-Vidal et at., 1975], Two binding constants of similar values were observed in cultured mouse TSH-secreting tumor cells [Grant et at., 1973], A previous autoradiographic study together with a chemical analysis of intact GH3 cells previously incubated for 30 min at 37 C with ['H]-TRH and then thoroughly washed at 4 C. have demonstrated the presence of chemically unmodified TRH associated to subcellular fractions: nucleus (17%), cytoplasmic organelles (26%) and cytosol (57%) [Gourdji et ah, 1973: Brunet et a/., 1974. It was.…”
mentioning
confidence: 99%