Chemical Identification and Antioxidant Screening of Bufei Yishen Formula using an Offline DPPH Ultrahigh-Performance Liquid Chromatography Q-Extractive Orbitrap MS/MS

Wu, Jinyan; Cai, Bangrong; Zhang, Ang; Zhao, Peng; Du, Yan; Liu, Xuefang; Zhao, Di; Yang, Liu; Liu, Xinguang; Li, Jiansheng

doi:10.1155/2022/1423801

Cited by 6 publications

(4 citation statements)

References 47 publications

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“…Flavonoids have strong antioxidant properties, and their antioxidant effect is related to the number of phenolic hydroxyl groups in the B ring and the adjacent phenolic hydroxyl groups and the aromatic structures . Based on the above structures, quercetin, rutinum, luteolin, myricetin, naringenin, and calycosin have been reported to have significant antioxidant effects. Thus, we chose a natural stabilizer of 5-MTHF from the six different flavonoid monomers mentioned above and compared it to the traditional stabilizers.…”

Section: Resultsmentioning

confidence: 99%

Naringenin as a Novel Stabilizer for Improving the Stability of 5-Methyltetrahydrofolate

Zhou,

Xu,

Hou

et al. 2024

ACS Food Sci. Technol.

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This study discovered a natural flavonoid stabilizer for stabilizing 5-methyltetrahydrofolate (5-MTHF) since the instability of 5-MTHF affects the accurate determination of 5-MTHF. The optimal stabilizer was selected from six flavonoid compounds, comparing the differences between natural stabilizers and traditional stabilizers and studying the parameters of the stabilizing 5-MTHF solution. In addition, the stabilizing effect of the stabilizer on 5-MTHF was evaluated under optimal parameters under various environmental conditions. Differences in the methods with and without naringenin were compared. The concentrations of 5-MTHF were analyzed using validated liquid chromatography-tandem mass spectrometry, and percentage deviation characterized the stability. The results indicated that naringenin had a better stabilizing effect on 5-MTHF compared to dithiothreitol, similar to l-ascorbic acid, maintaining the stability of 5-MTHF under different conditions. Naringenin had no adverse effect on the stability of other metabolites. In conclusion, naringenin improved the stability of 5-MTHF, ensuring its stability during the storage and detection processes.

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Section: Resultsmentioning

confidence: 99%

Naringenin as a Novel Stabilizer for Improving the Stability of 5-Methyltetrahydrofolate

Zhou,

Xu,

Hou

et al. 2024

ACS Food Sci. Technol.

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“…Its high efficiency was further supported by Wu’s work which simultaneously identified 189 compounds from Bufei Yishen Formula. However, Wu’s work failed to offer a full MS spectrum elucidation of all compounds and also to distinguish isomers [ 9 ]. Therefore, Wu’s work could only be considered as a tentative identification and our work as a putative identification.…”

Section: Resultsmentioning

confidence: 99%

Detection of Adulterated Naodesheng Tablet (Naodesheng Pian) via In-Depth Chemical Analysis and Subsequent Reconstruction of Its Pharmacopoeia Q-Markers

Li,

Zeng

et al. 2024

Molecules

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Naodesheng Tablet (Naodesheng Pian), a traditional Chinese medicine formula for stroke treatment, is made up of five herbal medicines, i.e., Sanqi, Gegen, Honghua, Shanzha, and Chuanxiong. However, the current Pharmacopoeia quality-marker (Q-marker) system cannot detect possible adulteration. Our study tried to use a new strategy, i.e., standards-library-dependent ultra-high-performance liquid chromatography-quadrupole-Orbitrap mass spectrometry (UHPLC-Q-Orbitrap MS/MS) putative identification, to reconstruct the Q-marker system. Through the strategy, 30 isomers were successfully differentiated (such as 2′-hydroxygenistein, luteolin, and kaempferol; ginsenoside Rg2 and ginsenoside Rg3; ginsenoside Rf and ginsenoside Rg1). In particular, 11 compounds were unexpectedly found in Naodesheng, including 2′-hydroxygenistein, 7,4′-dihydroxyflavone, pectolinarigenin, 7-methoxy-4′-hydroxyisoflavone, scoparone, matrine, 3,3′,4′,5,6,7,8-heptamethoxyflavone, 5-hydroxyflavone, diosgenin, chloesteryl acetate, and (+)-4-cholesten-3-one. In total, 68 compounds were putatively identified and fully elucidated for their MS spectra. Subsequently, relevant compounds were further investigated using UV-vis scanning experiments, semi-quantitative analysis, and quantum chemical calculation. Finally, five adulterated Naodesheng Tablets were used for validation experiments. The experiment successfully detected five adulterated ones via a lower-version LC-MS analysis. On this basis, three new candidates (hydroxy safflor yellow A (HSYA), citric acid, and levistilide A), along with puerarin and notoginsenoside R1, are re-nominated as the Q-markers for LC-MS analysis. The LC-MS analysis of puerarin, notoginsenoside R1, HSYA, citric acid, and levistilide A can clearly detect adulteration regarding all five herbal medicines mentioned above. Therefore, the reconstructed Q-markers are described as a “perfect” quality control system to detect adulteration in Naodesheng and will offer a valuable recommendation for the Pharmacopoeia Commission.

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“…COPD rats were administered the powder orally (10 ml/kg). In a previous study, UHPLC Q-Extractive Orbitrap-MS/MS was used to characterize the chemical constituents of the BYF extract [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

Bufei Yishen formula protects the airway epithelial barrier and ameliorates COPD by enhancing autophagy through the Sirt1/AMPK/Foxo3 signaling pathway

Jia,

Liu,

Liu

et al. 2024

Chin Med

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Object Bufei Yishen formula (BYF), a traditional Chinese medicine alleviates COPD symptoms and suppresses airway epithelial inflammation. In this study, we determined whether BYF protects the airway epithelial barrier from destruction in COPD rats. Methods The protective effects of BYF on the airway epithelial barrier were examined in a rat COPD model. BEAS-2B epithelial cells were exposed to cigarette smoke extract (CSE) to determine the effect of BYF on epithelial barrier function. Transcriptomic and network analyses were conducted to identify the protective mechanisms. Results Oral BYF reduced the severity of COPD in rats by suppressing the decline in lung function, pathological changes, inflammation, and protected airway epithelial barrier function by upregulating apical junction proteins, including occludin (OCLN), zonula occludens (ZO)-1, and E-cadherin (E-cad). BYF treatment reduced epithelial permeability, and increased TEER as well as the apical junction proteins, OCLN, ZO-1, and E-cad in BEAS-2B cells exposed to CSE. Furthermore, 58 compounds identified in BYF were used to predict 421 potential targets. In addition, the expression of 572 differentially expressed genes (DEGs) was identified in CSE-exposed BEAS-2B cells. A network analysis of the 421 targets and 572 DEGs revealed that BYF regulates multiple pathways, of which the Sirt1, AMPK, Foxo3, and autophagy pathways may be the most important with respect to protective mechanisms. Moreover, in vitro experiments confirmed that nobiletin, one of the active compounds in BYF, increased apical junction protein levels, including OCLN, ZO-1, and E-cad. It also increased LC3B and phosphorylated AMPK levels and decreased the phosphorylation of FoxO3a. Conclusions BYF protects the airway epithelial barrier in COPD by enhancing autophagy through regulation of the SIRT1/AMPK/FOXO3 signaling pathway.

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Chemical Identification and Antioxidant Screening of Bufei Yishen Formula using an Offline DPPH Ultrahigh-Performance Liquid Chromatography Q-Extractive Orbitrap MS/MS

Cited by 6 publications

References 47 publications

Naringenin as a Novel Stabilizer for Improving the Stability of 5-Methyltetrahydrofolate

Naringenin as a Novel Stabilizer for Improving the Stability of 5-Methyltetrahydrofolate

Detection of Adulterated Naodesheng Tablet (Naodesheng Pian) via In-Depth Chemical Analysis and Subsequent Reconstruction of Its Pharmacopoeia Q-Markers

Bufei Yishen formula protects the airway epithelial barrier and ameliorates COPD by enhancing autophagy through the Sirt1/AMPK/Foxo3 signaling pathway

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