Chemical imaging of living cells by synchrotron infrared microspectrometry

Jamin, Nadège; Dumas, Paul; Moncuit, Janine; Fridman, Wolf-Herman; Teillaud, Jean‐Luc; Carr, G. L.; Williams, Gwyn

doi:10.1117/12.279380

Cited by 11 publications

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“…23,31,32 The IR region of the emitted synchrotron radiation can be directed into a benchtop FT-IRM S and used as a source instead of conventional blackbody sources. 58 This high brightness of synchrotron sources enables much smaller regions to be probed with an acceptable Signal to Noise ratio (S/N) thus, 45 greater spatial resolution approaching the diffraction limit can be achieved.…”

Section: Comparison Of Control and Ec23 Treated Cells After 7 Days Usmentioning

confidence: 99%

The action of all-trans-retinoic acid (ATRA) and synthetic retinoid analogues (EC19 and EC23) on human pluripotent stem cells differentiation investigated using single cell infrared microspectroscopy

et al. 2013

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(2013) 'The action of all-trans-retinoic acid (ATRA) and synthetic retinoid analogues (EC19 and EC23) on human pluripotent stem cells di erentiation investigated using single cell infrared microspectroscopy.', Molecular bioSystems., 9 (4). pp. 677-692. Further information on publisher's website:http://dx.doi.org/10.1039/c3mb25505kPublisher's copyright statement:Additional information: Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-pro t purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details.

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Section: Comparison Of Control and Ec23 Treated Cells After 7 Days Usmentioning

confidence: 99%

The action of all-trans-retinoic acid (ATRA) and synthetic retinoid analogues (EC19 and EC23) on human pluripotent stem cells differentiation investigated using single cell infrared microspectroscopy

et al. 2013

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“…This advantage has opened up the capability of imaging the chemical components of single living cells in much higher contrast (down to the diffraction limit). 6,7 Chemical mappings of nucleic acids, proteins, and lipids in single living mouse hybridoma B cells at a spatial resolution of 3 m have been achieved using this approach. 6,7 Apoptosis describes a type of programmed cell death (PCD), which is different from necrosis and defined by major morphological changes: cell shrinkage, membrane blebbing, and chromatin condensation.…”

Section: Introductionmentioning

confidence: 99%

“…In the present work, apoptosis was induced by anti-Fas mAbs because of it is one of the most often used and best examined models of apoptosis. Two cell lines from two different mammalian species were used in our experiments: a mouse hybridoma B cell line (LS 102.9), which is a cell type that was used in our initial experiments, 6,7 and a human T cell line (Jurkat) that is commonly used for studies on apoptosis. An inducible model of apoptosis based on the use of two anti-Fas/ CD95 mAbs (Jo2 anti-mouse Fas mAb or DX2 anti-human Fas mAb) and Fas ϩ LS 102.9 or Jurkat cells was first set up.…”

Section: Introductionmentioning

confidence: 99%

Chemical heterogeneity in cell death: Combined synchrotron IR and fluorescence microscopy studies of single apoptotic and necrotic cells

et al. 2003

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The combination of synchrotron IR microspectroscopy and fluorescence microscopy has led to the identification of specific IR signatures of apoptosis and necrosis at a single cell level. Apoptosis was induced by treatment of Fas+ tumor cell lines with anti-Fas monoclonal antibodies. Detection of the early and late stages of apoptosis was performed using conjugated annexin V-fluorescein isothiocyanate (AV-FITC) and propidium iodide. Very early cellular changes were detected by IR before externalization of phosphatidylserine and AV-FITC labeling, and they were probably linked to DNA unwinding. The IR signals at 1044, 1177, and 1222 cm(-1), as well as an intensity variation in the CHx stretching region, are the main signature changes of early and late apoptosis, in line with the hypothesis of DNA fragmentation. The increased intensity of the CHx stretching bands of the lipids was observed only at an early stage of apoptosis. Changes in the relative intensity of CH3 and CH2 stretching accompany this increased intensity, suggesting changes in the relative amount and/or type of lipids concomitant with an increased lipid content. Finally, necrotic cells were characterized by marked changes in their chemical composition because several new vibrational features were observed.

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“…Fourier transform infrared (FTIR) spectroscopy has been demonstrated as a potential tool for disease diagnostics in tissue [1][2][3] and in its microscopical form, for cellular analysis [4][5][6][7][8][9][10][11][12]. The use of synchrotron sources and attenuated total reflection imaging have achieved subcellular resolution thus promising a 20 powerful technique for the analysis of the biochemical origin of disease [7,13,14].…”

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Reflection contributions to the dispersion artefact in FTIR spectra of single biological cells

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et al. 2009

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Fourier transform infrared spectra of a single cell in transflection geometry are seen to vary significantly with position on the cell, showing a distorted derivative-like lineshape in the region of the optically dense nucleus. A similar behaviour is observable in a model system of the protein albumin doped in a potassium bromide disk. It is demonstrated that the spectrum at 10 any point is a weighted sum of the sample reflection and transmission and that the dominance of the reflection spectrum in optically dense regions can account for some of the spectral distortions previously attributed to dispersion artefacts. Rather than being an artefact, the reflection contribution is ever present in transflection spectra and it is further demonstrated that the reflection characteristics can be used for cellular mapping.

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Chemical imaging of living cells by synchrotron infrared microspectrometry

Abstract: Chemical mapping of proteinszmd lipids inside a single living cell and at a resolution of a few microns, has been perfbrmed using .synchroton infrared microspectrometry. Modifications of the chemical distributions upon mitosis and necrosis has been investigated.

Cited by 11 publications

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The action of all-trans-retinoic acid (ATRA) and synthetic retinoid analogues (EC19 and EC23) on human pluripotent stem cells differentiation investigated using single cell infrared microspectroscopy

The action of all-trans-retinoic acid (ATRA) and synthetic retinoid analogues (EC19 and EC23) on human pluripotent stem cells differentiation investigated using single cell infrared microspectroscopy

Chemical heterogeneity in cell death: Combined synchrotron IR and fluorescence microscopy studies of single apoptotic and necrotic cells

Reflection contributions to the dispersion artefact in FTIR spectra of single biological cells

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