Chemical Immobilization of Biomolecules on Plasma‐Modified Substrates for Biomedical Applications

Lopez, Linda C.; Gristina, Roberto; d’Agostino, Riccardo; Favia, Pietro

doi:10.1002/9783527622184.ch14

Cited by 2 publications

(1 citation statement)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most of these methods were first developed for materials devoted to biomedical applications, in particular for implants aimed at replacing or restoring the function of compromised or degenerated tissues or organs [ 23 , 24 , 25 , 26 ]. Among all techniques utilized for engineering surfaces, those based on plasma technology have been proven to be extremely efficient in defining surface properties simply by varying the plasma parameters [ 27 , 28 , 29 , 30 , 31 ]. In this work, plasma processes have been explored as an alternative method with respect to amino silanes for introducing amino groups on silicon surfaces with the aim of capturing microRNA biomarkers.…”

Section: Introductionmentioning

confidence: 99%

Tuning Surface Properties via Plasma Treatments for the Improved Capture of MicroRNA Biomarkers

et al. 2022

Self Cite

View full text Add to dashboard Cite

Advanced materials could bring about fundamental improvements in the evolution of innovative analytical devices, i.e., biosensors or lab-on-a-chip devices, in particular in the context of liquid biopsies. Here, plasma deposition processes were tested for the introduction of primary amines on silicon surfaces by tuning the amounts and availability of amino-charged residues. Different binary (CH4/NH3) and ternary (CH4/NH3/H2 and CH4/NH3/N2) mixtures of gases were used as feeds for the plasma treatments. The obtained surfaces were fully characterized for their chemical and physical properties before their use as capture materials in a functional test. Synthetic and fluorescently conjugated microRNA-21 (miR-21) was selected as the target molecule. The capture of miR-21 increased linearly with the increase in amino nitrogen measured on surfaces. The surface showing the most promising performance was further analyzed in different conditions, i.e., varying pH and time of incubation, incubation with different microRNAs, and possible elution of captured microRNAs. The apparent pH range of primary amines present on the surfaces was around 3.5–4. Positively charged surfaces prepared via PE-CVD were, therefore, demonstrated as being suitable materials for the capture of microRNA biomarkers, paving the way for their inclusion in biomedical devices for the purification and analysis of circulating biomarkers.

show abstract