Chemical Induction of Endogenous Retrovirus Particles from the Vero Cell Line of African Green Monkeys

Ma, Hailun; Ma, Yunkun; Ma, Wenbin; Williams, Dhanya K.; Galvin, Teresa A.; Khan, Arifa S.

doi:10.1128/jvi.00147-11

Cited by 29 publications

(25 citation statements)

References 71 publications

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“…PCR-amplified DNA fragments were isolated and sequenced as previously described (6). Sequence analysis was done using the BLAST (http://blast.ncbi.nlm.nih.gov/) and the Pfam (version 27.0; http://pfam.sanger.ac.uk/) programs.…”

Section: Methodsmentioning

confidence: 99%

Identification of a Novel Rhabdovirus in Spodoptera frugiperda Cell Lines

Galvin

Glasner

et al. 2014

J Virol

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127

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The Sf9 cell line, derived from Spodoptera frugiperda, is used as a cell substrate for biological products, and no viruses have been reported in this cell line after extensive testing. We used degenerate PCR assays and massively parallel sequencing ( IMPORTANCEThe Spodoptera frugiperda Sf9 cell line is used as a cell substrate for the development and manufacture of biological products. Extensive testing has not previously identified any viruses in this cell line. This paper reports on the identification and characterization of a novel rhabdovirus in Sf9 cells. This was accomplished through the use of next-generation sequencing platforms, de novo assembly tools, and extensive bioinformatics analysis. Rhabdovirus identification was further confirmed by transmission electron microscopy. Infectivity studies showed the lack of replication of Sf-rhabdovirus in human cell lines. The overall study highlights the use of a combinatorial testing approach including conventional methods and new technologies for evaluation of cell lines for unexpected viruses and use of comprehensive bioinformatics strategies for obtaining confident next-generation sequencing results.

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Section: Methodsmentioning

confidence: 99%

Identification of a Novel Rhabdovirus in Spodoptera frugiperda Cell Lines

Galvin

Glasner

et al. 2014

J Virol

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127

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“…Our first challenge was to recreate an endogenous RT activity since these enzymes displaying RT activity are currently undetermined. Demethylating agent exposure to various cell lines is able to induce such RT activities [22]. Amongst them, azacytidine (AzaC) at 1,25 μg/mL and/or desoxiuridine (IdU) at 200 μg/mL, allowed the detection RNA:DNA duplexes in the cytosol of cultured keratinocytes within 48 h (Fig 3A and 3B).…”

Section: Resultsmentioning

confidence: 99%

Cytosolic RNA:DNA Duplexes Generated by Endogenous Reverse Transcriptase Activity as Autonomous Inducers of Skin Inflammation in Psoriasis

Molès¹,

Griez²,

Guilhou³

et al. 2017

PLoS ONE

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Psoriasis is a chronic skin disease of unknown ætiology. Recent studies suggested that a large amount of cytosolic DNA (cyDNA) in keratinocytes is breaking keratinocytes DNA tolerance and promotes self-sustained inflammation in the psoriatic lesion. We investigated the origin of this cyDNA. We show that, amongst all the possible DNA structures, the cyDNA could be present as RNA:DNA duplexes in keratinocytes. We further show that endogenous reverse transcriptase activities generate such duplexes and consequently activate the production of Th1-inflammatory cytokines. These observations open a new research avenue related to endogenous retroelements for the aetiology of psoriasis and probably of other human chronic inflammatory diseases.

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“…Cellular RNAs were tested for endogenous retrovirus activation using virus-specific PCR assays and filtered supernatants were analyzed for virus particle production using a highly-sensitive RT assay and PCR assays [1]. Infectivity studies were done using various cell lines from different species based upon their susceptibility to known retrovirus infections.…”

Section: Methodsmentioning

confidence: 99%

Optimization of chemical induction for evaluation of endogenous retroviruses in different species

et al. 2011

Retrovirology

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Chemical Induction of Endogenous Retrovirus Particles from the Vero Cell Line of African Green Monkeys

Cited by 29 publications

References 71 publications

Identification of a Novel Rhabdovirus in Spodoptera frugiperda Cell Lines

Identification of a Novel Rhabdovirus in Spodoptera frugiperda Cell Lines

Cytosolic RNA:DNA Duplexes Generated by Endogenous Reverse Transcriptase Activity as Autonomous Inducers of Skin Inflammation in Psoriasis

Optimization of chemical induction for evaluation of endogenous retroviruses in different species

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