Chemical labeling and crosslinking of tobacco mosaic virus via multi-diazonium reagents: examples, applications, and prospects

Abstract

Tobacco mosaic virus (TMV) is a rod-shaped hollow plant viral nanoparticle (300 nm × 18 nm) and exhibits abundant amino acid residues on its surface of capsid proteins for facile...

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“…The bio‐orthogonal functional groups such as azide, alkyne, aldehyde, hydrazide, or alkoxyamine are of great interest for click and oxime reactions based on bioconjugation chemistry (Kölmel & Kool, 2017; McKay & Finn, 2014). Catalysis of reaction between bio‐orthogonal functional groups catalyzed by Cu (I), termed as Cu(I)‐catalyzed azide‐alkyne cycloaddition (CuAAC), diazonium salts and reagents has been widely used for highly efficient bioconjugation (Qiu et al, 2022). The use of these methods for modulating the surface properties of TMV has been successfully used to construct new materials (Bruckman et al, 2008; Qiu et al, 2022; Schlick et al, 2005).…”

“…Catalysis of reaction between bio‐orthogonal functional groups catalyzed by Cu (I), termed as Cu(I)‐catalyzed azide‐alkyne cycloaddition (CuAAC), diazonium salts and reagents has been widely used for highly efficient bioconjugation (Qiu et al, 2022). The use of these methods for modulating the surface properties of TMV has been successfully used to construct new materials (Bruckman et al, 2008; Qiu et al, 2022; Schlick et al, 2005). For instance, TMV‐based hydrogels based on dual‐diazonium reagents could be used to encapsulate small molecules for sustained release (Ma et al, 2018; Q. Zhang et al, 2019).…”