Chemical management in fungicide sensivity of Mycosphaerella fijiensis collected from banana fields in México

Aguilar-Barragan, Alejandra; García-Torres, Ana Elisa; Odriozola-Casas, Olga; Macedo-Raygoza, Gloria; Ogura, Tetsuya; Manzo-Sánchez, Gilberto; James, Andrew; Islas‐Flores, Ignacio; Beltrán-García, Miguel J.

doi:10.1590/s1517-83822014000100051

Cited by 16 publications

(14 citation statements)

References 22 publications

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“…This is in agreement with previous work showing that the majority of resistant strains was isolated from countries where the banana production is economically very important, such as Costa Rica and Ecuador, and where fungicide application frequencies are very high (Aguilar-Barragan et al 2014;Amil et al 2007;Arango et al 2016;Chong et al 2016b;Marín et al 2003).…”

Section: Discussionsupporting

confidence: 92%

The origin, versatility and distribution of azole fungicide resistance in the banana black Sigatoka pathogen Pseudocercospora fijiensis

Aguirre

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Section: Discussionsupporting

confidence: 92%

The origin, versatility and distribution of azole fungicide resistance in the banana black Sigatoka pathogen Pseudocercospora fijiensis

Aguirre

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“…Due to the lack of effective host resistance, management of this fungus is based on frequent fungicide applications. Weekly applications in most banana plantations lead to rapid evolution of fungicide–resistant populations (Aguilar-Barragan et al, 2014). Given its importance for food security and likely side effects on human health from pesticide residues on fruits, we need an urgent eco-friendly strategy for protecting banana crops.…”

Section: Introductionmentioning

confidence: 99%

Enterobacter cloacae, an Endophyte That Establishes a Nutrient-Transfer Symbiosis With Banana Plants and Protects Against the Black Sigatoka Pathogen

et al. 2019

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Banana ( Musa spp.) is an important crop worldwide, but black Sigatoka disease caused by the fungus Pseudocercospora fijiensis threatens fruit production. In this work, we examined the potential of the endophytes of banana plants Enterobacter cloacae and Klebsiella pneumoniae , as antagonists of P. fijiensis and support plant growth in nutrient limited soils by N-transfer. The two bacterial isolates were identified by MALDI-TOF mass spectrometry and corroborated by 16S rRNA sequence analysis. Both bacteria were positive for beneficial traits such as N-fixation, indole acetic acid production, phosphate solubilization, negative for 1-aminocyclopropane 1-carboxylic acid deaminase and were antagonistic to P. fijiensis . To measure the effects on plant growth, the two plant bacteria and an E. coli strain (as non-endophyte), were inoculated weekly for 60 days as active cells (AC) and heat-killed cells (HKC) into plant microcosms without nutrients and compared to a water only treatment, and a mineral nutrients solution (MMN) treatment. Bacterial treatments increased growth parameters and prevented accelerated senescence, which was observed for water and mineral nutrients solution (MMN) treatments used as controls. Plants died after the first 20 days of being irrigated with water; irrigation with MMN enabled plants to develop some new leaves, but plants lost weight (−30%) during the same period. Plants treated with bacteria showed good growth, but E. cloacae AC treated plants had significantly greater biomass than the E. cloacae HKC. After 60 days, plants inoculated with E. cloacae AC showed intracellular bacteria within root cells, suggesting that a stable symbiosis was established. To evaluate the transference of organic N from bacteria into the plants, the 3 bacteria were grown with 15 NH 4 Cl or Na 15 NO 3 as the nitrogen source. The 15 N transferred from bacteria to plant tissues was measured by pheophytin isotopomer abundance. The relative abundance of the isotopomers m/z 872.57, 873.57, 874.57, 875.57, 876.57 unequivocally demonstrated that plants acquired 15 N atoms directly from bacterial cells, using them as a source of N, to support plant growth in restricted nutrient soils. E. cloacae might be a new alternative to promote growth and health of banana crops.

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“…The findings described herein therefore, regarding the existence of alternative hosts of P. fijiensis is very important for black Sigatoka management programs. Currently, many of the programs for the control of inoculum multiplication involve intensive, weekly applications of fungicides, more than 50 times a year [4,26]; however, control of initial inoculum receives less attention (e.g., detached, infected banana leaves are left on the soil to decay inside the plantation). For sustainable management programs, it is important to achieve control of initial inoculum as well.…”

Section: Discussionmentioning

confidence: 99%

Identification of New Hosts of Pseudocercospora fijiensis Suggests Innovative Pest Management Programs for Black Sigatoka Disease in Banana Plantations

et al. 2019

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Black Sigatoka is the main constraint to banana production worldwide, and epidemic outbreaks are continuously causing huge losses. Successful management of diseases requires a profound knowledge of the epidemiological factors that influence disease dynamics. Information regarding alternative hosts of Pseudocercospora fijiensis, the causal agent, is still very scarce. To date, only Heliconia psittacorum has been reported as an alternative plant host, and we hypothesized that other plants can house P. fijiensis. In the present report, ten plant species with suspicious leaf spots were collected inside and around commercial banana crops in Mexico. Diagnostic PCR gave positive amplification for six of these plant species, and DNA sequencing confirmed the presence of the pathogen in four. This is the first report of the presence of P. fijiensis in unrelated plants and it represents a breakthrough in the current knowledge of black Sigatoka. This finding is very important given the polycyclic nature of this disease whose successful management requires the control of initial inoculum to minimize epidemic outbreaks. The results presented herein can be used to introduce innovations in integrated black Sigatoka management programs to reduce initial inoculum, and help the international initiative to reduce the use of fungicides in banana production.

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Chemical management in fungicide sensivity of Mycosphaerella fijiensis collected from banana fields in México

Cited by 16 publications

References 22 publications

The origin, versatility and distribution of azole fungicide resistance in the banana black Sigatoka pathogen Pseudocercospora fijiensis

The origin, versatility and distribution of azole fungicide resistance in the banana black Sigatoka pathogen Pseudocercospora fijiensis

Enterobacter cloacae, an Endophyte That Establishes a Nutrient-Transfer Symbiosis With Banana Plants and Protects Against the Black Sigatoka Pathogen

Identification of New Hosts of Pseudocercospora fijiensis Suggests Innovative Pest Management Programs for Black Sigatoka Disease in Banana Plantations

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